Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.3295.3305Fasciola gigantica Eggs with Reference to its Effect on Clostridium oedematiens and Correlation to Chemical Composition]]>HegaziAhmed G.Abd El HadyFaten K.ShalabyHatem A.1220071019The chemical composition of the propolis samples was investigated by GC/MS and HPLC. 91 compounds have been identified, 26 compounds are being new to propolis. Siwa oasis propolis was characterized by the presence of diprenyl-dihydrocoumaric acids (4.15%), coumarate esters (10.93%), benzofuran lignans (13.47%) and valeric acids derivatives (11.42%). Matrooh sample was characterized by the presence of new ferulate esters, hydroxy acetophenones and furanon derivatives, furofuran lignans and sterol from pregnane type. Dakahlia propolis was a typical poplar propolis. The present study proved evidence of inhibitory activity of propolis on the vitality and hatchability of immature F. gigantica eggs, where three different localities in Egypt were tested for this purpose. The Siwa propolis showed the highest inhibitory effect than the other sources. The complete failure of development and death of all immature eggs were recorded at concentration of 200 μg mL-1 of Siwa, 400 μg mL-1 of Matrooh and 800 μg mL-1 of Dakahlia propolis. The exposed F. gigantica egg shell revealed the same features as the non exposed egg shell when scanning with the electron microscopy. The three propolis samples showed inhibition in the growth of associated bacterium Clostridium oedematiens (Clostridium novyi) type B to fascioliasis. The inhibition varied according to the propolis origin.]]>Abd El Hady, F.K. and A.G. Hegazi,200257386394Aga, H., T. Shibuye, T. Sugimoto, M. Kurimoto and S. Nakagima,199458945946Annual Book of ASTM Standards,1983Bagadi, H.O. and M.M. Sewell,1973154953Bankova, V.S., R.S. Chrostov and A.D. Tejera,19984914111415Banskota, A.H., Y. Tezuka, J.K. Prasain, K. Matsushige, I. Saika and S.H. Kadota,199861896900Banskota, A.H., Y. Tezuka, K. Midorikawa, K. Matsushige and S. Kadota,20006312771279Barron, D., G. Comte, G. Consil, J.B. Daskiewicz and C. Bayet et al.,19981998Boray, J.C.,19631963Boray, J.C.,19971997pp: 83-97pp: 83-97Christov, R., V.S. Bankova, I. Tsvetkova, A. Kujumgiev and A.D. Tejera,1999708992Comte, G., J.B. Daskiewicz, C. Bayet, G. Consil, A. Viornery-Vanbier, C. Dumontet and D. Barron,200144763768Cruickshank, R., J.P. Duguid, B.P. Masion and R.H. Swain,197912th Edn.,Dalton, J.P.,1999El-Badry, A.A.,19961996El-Sayad, M.H., A.F. Allam and A.Y. Shehab,200030655660Gil, M.I., F. Ferreres, A. Ortiz, E. Subra and F.A. Tomas-Barberan,19954328332838Greenaway, W., J. May, T. Scaysbrook and F.R.S. Whatley,199146111121Haseeb, A.N., A.M. El-Shazly, M.A. Arafa and A.T. Morsy,200232317354Hegazi, A.G., F. El-Berdiny, S. El-Assily, E. Khashabah, N. Hassan and S. Popov,1993415356Hegazi, A.G., N.Z. Moharm, F. Abd-Allah, E. El Sheikh, N.A. Ellithy and A.M. Khair,199810387396Hegazi, A.G., F.K. Abd El-Hady and F.A. Abd Allah,2000557075Hegazi, A.G. and F.K. Abd-El-Hady,2001568288Hegazi, A.G. and F.K. Abd El Hady,200257395402Higashi, K.O. and S.L. De-Castro,1994Trypanosoma cruzi and have an impact on its interaction with host cells.]]>43149155Kujumgiev, A., I. Tsvetkova, Y. Serkedjieva, V. Bankova, R. Christov and S. Popov,199964235240Marcucci, M.C.,1995268399Marcucci, M.C., F. Ferreres, C. Garcia-Viguera, V. Bankova and S.L. Decastro et al.,200174105112Matsuno, T., S.K. Jung, Y. Matsumoto, M. Saito and J. Morikawa,19971735653568Mertzner, J., H. Bekemeier, M. Paintz and E. Schneidewind,19793497102Moor, W.B.,1968Clostridium novyi type B.]]>53415415Ogan, K. and E. Katz,198153160163Park, Y.K., J.F. Paredes-Guzman, C.L. Aguiar, S.M. Alencar and F.Y. Fujiwara,2004Baccharis dracunculifolia as the main botanical origin of Southeastern Brazilian Propolis.]]>5211001103Roweliffe, S.A. and O.B. Ollerenshaw,1959F. hepatica.]]>54172181Rubio, O.C., A.C. Cuellar, N. Rojas, H.V. Castro, L. Rastrelli and R.A. Aquino,19996210131015Salatino, A., E.W. Teixeira, G. Negri and D. Message,200523338Senedcor, G.W., 19615th Edn.,Tomas-Barbaran, F., C. Garcia-Viguera, P. Vit-Olivier, F. Ferreres and F. Tomas-Lorente,199334191196Trusheva, B., M. Povova, H. Naydenski, I. Tsvetkova, J.G. Rodriguez and V. Bankova,200475683689Valcic, S., G. Montenegro and B.N. Timmermann,199861771775Valcic, S., G. Montenegro, A.M. Mujica, G. Avila and S. Franzblau et al.,199954406416Welch, R.D., P.H. Smith, J.B. Malone, R.A. Holmes and J.P. Geaghan,1987Fasciola hepatica infection in Louisiana cattle by an ELISA.]]>48345347