Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.2465.2469Staphylococci in Bovine Clinical Mastitis]]>MoniriR. DastehgoliK. AkramianA. 1220071015The aim of this study was to determine Coagulase Negative Staphylococci (CNS) and other bacteria for their resistance to antimicrobial agents approved for the control of pathogens involved in clinical bovine mastitis. This descriptive study was done on 106 milk samples obtained from clinical mastitis in dairy cattle husbandry from April 2006 through August 2006 in Kashan, Iran. From the total of 106 milk samples collected from clinical mastitis, 96 (90.6%) lead to positive culture. Coagulase negative Staphylococci isolated in 51 out of 96 samples (53.1%), Staphylococcus aureus isolated in 21 out of 96 (21.9%), gram negative bacilli isolated in 14 out of 96 (14.6%) and Enterococci isolated in 4 (4.2%). The highest rate of resistant CNS observed to penicillin (56.6%) and the highest rate of sensitivity to enrofloxacin 100%, followed by kanamycin, streptomycin and neomycin, 92.2, 82.3 and 82.3%, respectively. The highest rate of resistance S. aureus exhibited to penicillin (66.6%); while the highest rate of sensitivity showed to trimethoprim-sulphamethoxasole (81%), followed by kanamycin and enrofloxacin both at 76.2%. The highest rate of resistance gram negative bacilli exhibited to ampicillin and erythromycin at 71.4%. Their highest rate of sensitivity observed to enrofloxacin (78.6%), followed by kanamycin, (71.4%). In recent years, CNS is emerging as important minor mastitis pathogens and can be the cause of substantial economic losses. The high resistance rate to penicillin and other antibiotics found in this study emphasize the importance of identification of CNS when a bovine clinical mastitis is present.]]>Bennedsgaard, T.W., S.M. Thamsborg, F.M. Aarestrup, C. Enevoldsen, M. Vaarst and A.B. Christoffersen,2006Staphylococcus aureus isolates from cows with high somatic cell counts in organic and conventional dairy herds in Denmark.]]>482424Bergonier, D., R. de Cremoux, R. Rupp, G. Lagriffoul and X. Berthelot,200334689716Calvinho, L.F., F.G. Toselli, W.R. Weimann, V.R. Canavesio, V.E. Neder and I.A. Iguzquiza,200234171175Casadevall, A. and L.A. Pirofski,20006865116518Corti, S., D. Sicher, W. Regli and R. Stephan,2003145571575Fetrow, J.,20002000pp: 347Gentilini, E., G. Denamiel, A. Betancor, M. Rebuelto, M.R. Fermepin and R.A. De Torrest,20028519131917Gianneechini, R.E., C. Concha and A. Franklin,2002433141Harmon, R.J. and B.E. Langlois,1995Staphylococcus species.]]>1995pp: 5664Huxley, J.N., M.J. Green and A.J. Bradley,20022002pp: 129130Jones, T.O.,1990Escherichia coli mastitis in dairy cattle: A review of the literature.]]>60205220Kalmus, P., A. Viltrop, B. Aasmae and K. Kask,2006482121Kudinha, T. and C. Simango,2002736265Myllys, V., K. Asplund, E. Brofeldt, V. Hirvela-Koski and T. Honkanen-Buzalski et al.,199839119126NCCLS-National Committee for Clinical Laboratory Standards,1999National Mastitis Council,1987Owens, W.E., C.H. Ray, J.L. Watts and R.J. Yancey,199780313317Rajala-Schultz, P.J., K.L. Smith, J.S. Hogan and B.C. Love,20041023342Roesch, M., V. Perreten, M.G. Doherr, W. Schaeren, M. Schallibaum and J.W. Blum,200689989997Smith, K.L. and J.S. Hogan,19939489498Sordillo, L.M. and N.L. Scott,19951995Tenhagen, B.A., G. Koster, J. Wallmann and W. Heuwieser,20068925422551Waage, S., J. Bjorland, D.A. Caugant, H. Oppegaard, T. Tollersrud, T. Mørk and F.M. Aarestrup,2002Staphylococcus aureus resistant to penicillin and tetracycline within and between dairy herds.]]>129193202Watts, J.L., S.A. Salmon, R.J. Jr. Yancey, S.C. Nickerson and L.J. Weaver et al.,19957816371648