Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.2020.2024+/H+ Antiporter Enhance Salt Stress in Zea mays L. Seedlings]]>TajdoostS. FarboodniaT. HeidaryR. 1220071012The aim of this study to show the importance of salt intracellular compartmentation as a tolerance mechanism by inhibition of Na+/H+ antiporter system. In this research 4 day/old Zea mays L. seedlings (var. single cross 704) were exposed to 200 and 300 mM NaCl with and without 100 and 200 micromolar amiloride. After 48 h, the roots and shoots of seedlings were harvested separately. The changes of total Na+absorption, the amount of malondialdehyde and the activity of antioxidant enzymes such as guaiacol peroxidase, ascorbate peroxidase and catalase were analysed. The results indicated that Na+ absorption has been increased by salt stress but was not influenced by amiloride. Malondialdehyde content and the activity of antioxidant enzymes such as guaiacol peroxidase, ascorbate peroxidase and catalase were increased in salt stressed plants specially in plants treated with salt and amiloride. Therefore salt stress has caused osmotic and oxidative stress in plants and amiloride as inhibitor of vacuolar Na+/H+ antiporter has been increased salt stress. Therefore we concluded that Na+ compartmentation in the cell is very important to reduce its damage in the cytosol and vacuolar Na+/H+ antiporter has an essential role in Na+ homeostasis in the cell by exporting excess Na+ to the vacuole.]]>Ashraf, M. and P.J.C. Harris,2004166316Beaudoin, N., C. Serizet, F. Gosti and J. Giraudat,20001211031116Blumwald, E., G.S. Aharon and M.P. Apse,20001465140151Chen, G.X. and K. Asada,198930987998Prochazkova, D., R.K. Sairam, G.C. Srivastava and D.V. Singh,2001161765771Darley, C.P., O.C. van Wuytswinkel, K. van der Woude, W.H. Mager and A.H de Boer,2000Arabidopsis thaliana and Saccharomyces cerevisiae NHX1 genes encode amiloride sensitive electroneutral Na+/H+exchangers.]]>351241249Gruwel, M.L.H., V.L. Rauw, M. Loewin and S.R. Abrams,200113p and 23Na NMR system to study salt tolerance.]]>160785794Harinasut, P., D. Poonsopa, K. Roengmongkol and R. Charoensataporn,200329109113Hasegawa, P.M., R.A. Bressan, J.K. Zhu and H.J. Bohnert,200051463499Heath, R.L. and L. Packer,1968125189198Hong, Z., K. Lakkineni, Z. Zhang and D.P.S. Verma,20001-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress.]]>12211291136Yong, K., S. Shin, R. Nilda., T. Hwang, O. Han, B. Cho, S. Jung and O. Guh,20034321092117Maehly, A.C. and B. Chance,19591959pp: 357-425pp: 357-425Parida, A.K. and A.B. Das,200560324349Qiu, Q.S., B.J. Barkla, R. Vera-Estrella, J.K. Zhu and K.S. Schumaker,2003+/H+ exchange activity in the plasma membrane of Arabidopsis.]]>13210411052De Azevedo-Neto, A.D., J.T. Pitsco, J. Eneas-Filho, C.E.B. de Abreu and E. Gomes-Filho,2006568794