Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.1996.2001SoloukiMahmood NazhadNafiseh RigiVignaniRita SiahsarBarat AliKamaladiniHossain EmamjomehAbassali 1220071012Grapevines have Bronze ages archive in Sistan area of Iran. In order to study the genetic variation and taxonomic relationships between 6 cultivars of the Sistan grapevines (Vitis vinifera L.) at molecular level, random amplified polymorphic DNA (RAPD) markers were used. The data were subjected to statistical analyses and genetic resemblance was calculated using Dice similarity index. The grapevines related to the different geographic areas of Sistan were assessed by 50 short (10 mer) and long (15-21 mer) primers. Out of 50 primers which were tested, 21 primers gave reproducible results. Selected primers created 497 bands. Resulting profiles showed that the produced bands varied in size from 300 to 3500 base pairs. The numbers of reliable polymorphic fragments for short and long primers were 86 and 334 bands, respectively. In multiplication reaction the items in the size area of 564 to 1904 base pair resulted for short primers and 564 to 4277 base pair for long primers. From the bands calculated a matrix that was analyzed by the unweighted pair group method on arithmetic averages to draw a dendrogram. The population was classified in 4 main groups in which Red Yaghooti and White Yaghooti had the maximum and Red Yaghooti and Laal had the minimum similarity coefficients. In our study, by comparing the results gained from technique long and short primers in RAPD, the potential value of long primers for the production of polymorphism in grapes was identified.]]>Adiguzel, A., G. Agar, O. Baris, M. Gulluce, F. Sahin and M. Sengul,2006Astragolus species growing in eastern Antatolia region of Turkey.]]>34424432Caetano-Anolles, G., B.J. Bassam and P.M. Gresshoff,19921992Castiglione, S., G. Wang, G. Damiani, C. Bandi, S. Bisoffi and F. Sala,1993Populus spp.) clones.]]>875459Collins, G.G. and R.H. Symons,199311105112Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Do, N. and R.P. Adams,199110162166Dolezalova, I., A. Lebeda, M. Dziechciarkova, E. Kristkova, D. Astley and C. Wiel,2003395967Eshraghi, P., R. Zarghami and H. Ofoghi,20069111114Fanizza, G., G. Colonna, P. Resta and G. Ferrara,1999Vitis vinifera.]]>1074550Galet, P.,1979Grando, M.S., L. De Micheli and A. Scienza,1996Vitis germplasm using random amplified polymorphic DNA markers.]]>43187192Javouhey, M., F. Daguin and R. Letouze,2000Phoenix dactylifera L.) industrial original offshoots and regenerated plantlets by RAPD markers.]]>530237242Kumar, S., V. Singh, M. Singh, Sh. Rai and S. Kumar et al.,2007Capsicum annuum L.).]]>111197202Lodhi, M.A., G.N. Ye, N.F. Weeden and B.I. Reisch,1994Vitis species. ]]>12613Lodhi, M.A., N.F. Weeden and B.I. Reich,199736133140Malabadi, R.B., P.N. Hills and J. van Staden,2006Pinus patula trees.]]>72181183Mantel, N.,196727209220Momeni, S.B.S. and K. Razmjoo,2006918981904Moreno, S., Y. Gogorcena and J.M. Ortiz,1995Vitis vinifera L.).]]>62237243Mullins, M.G., A. Bouquet and L.E. Williams,20005th Edn.,Murashige, T. and F. Skoog,196215473497Nei, M. and W. Li,19797952695273Newbury, H.J. and J.V. Possingham,197760543547Ohmi, C., A. Wakana and S. Shiraishi,199365195202Park, Y.H. and R.J. Kohel,199416652656Rafalski, J.A., S.V. Tingey and J.G.K. William,19913645648Rajput, S.G., K.J. Wable, K.M. Sharma, P.D. Kubde and S.A. Mulay,20065108112Rani, V., A. Parida and S.N. Raina,1995Populus deltoids Marsh.]]>14459462Rohlf, F.,1998Version 2.2,Schierwater, B. and A. Ender,19932146474648Shehata, M.M.,2004Triticum vulgare L.) cultivars.]]>7984994Shiran, B., N. Amirbakhtiar, S. Kiani, S. Mohammadi, B.E. Sayed-Tabatabaei and H. Moradi,2007111280292Stavrakakis, M.N., K. Biniari and K. Hatzopoulos,1997Vitis vinifera L.) by random amplified polymorphic DNA markers.]]>36175178Swanepoel, J.J. and C.E. Devilliers,1987Vitis spp. and cultivars based on leaf characteristics.]]>83135Ulanovsky, S., Y. Gogorcena, F.M. de Toda and J.M. Ortiz,2002Vitis vinifera L.).]]>92241254Welsh, J. and M. McClelland,19901872137218Ye, G.N., M. Hemmat, M.A. Lodhi, N.F. Weeden and B.I. Reisch,199620368371Yoon, C.S. and D.A. Glawe,199314908910Yu, C., S. Hu, H. Zhao, A. Guo and G. Sun,2005Brassica napus L.).]]>110511518