Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.1947.1955Astragalus gossypinus by Eco-Phytosociological Method from West of Iran]]>AtriMorteza NematianMahtab AsgariShahgolzariMehdi 1220071012 Astragalus gossypinus Fischer with wide distribution in Iran belongs to the genus Astragalus (Fabaceae). According to existing references and information, individuals of this species are present in many stations with different ecological conditions. This study carried out for determination and discrimination of intraspecific diversity of Astragalus gossypinus by Eco-phytosociological method from west of Iran. In this method, the principle of data collecting and analyzing based on floristical composition (as floristical markers) of each Endogenous milieu (as the unit of study in Eco-phytosociological method). In this order, application of Endogenous milieu (special station) for data collecting and then their analyzing permit us only determine existence of inter and intraspecific diversity. Then for determinating kind and level of intraspecific diversity (Ecophene, Chemotype, Cytotype, Ecotype ), can use other studies such as: morphological, anatomical, phytochemical, cytological and etc. In this survey, 29 special stations were studied and 195 species distinguished as companions for Astragalus gossypinus. Then floristic-ecologic data collected from each 29 special stations and analyzed by Anaphyto software (F.C.A, A.H.C, B.O, Marquag methods). Comparison of obtained results on multiple coordinate axes from F.C.A method with results from B.O, Marquag and A.H.C methods led to determination of 7 main groups of Endogenous milieus (special station). Flavonoid analyses were used for determinating kind and level of intraspecific diversity in 7 discriminated groups. Leaves flavonoid components of all collected individuals of Astragalus gossypinus were investigated by TLC method. Obtained data from flavonoid survey analyzed by SPSS and MVSP package with WARD and UPGMA methods. Finally, the results of flavonoid studies confirmed the same 7 groups that identified by floristical composition study and showed intraspecific diversity in chemotype level. So according to these results, we can introduce 7 chemotypes for Astragalus gossypinus from west of Iran. These chemotypes exist in different stations with various ecological conditions.]]>Atri, M.,19961996Atri, M.,199986173Atri, M. and M. Asgari Nematian,20062006Briane, J.P.,19951995Cain, P.D.,1959pp: 325pp: 325Fakhre-Tabatabaei, S.M., M. Atri and Ramakmaasoumi,2000Triticum boeoticum ssp. Thaoudar and its associates (Aegilops ssp.) in Iran.]]>32317322Joseph, O., B. Adil, H. Rebecca, G. Margaret, S. Juliana and W. Neil,2003Cruciferous species, Camelina sativa, Crambe ssp., Thiaspi arvense and several other genera of the family Brassicaceac.]]>3113091322Kalvandi, R., F. Sefidkon, M. Atri and M. Mirza,2004Thymus eriocalyx from Iran.]]>19341343Koch, M. and K.G. Bernhardt,2004Microthlaspi perfoliatum (Brassicaceae) in Europe using isozymes and cpDNA data: Refugia, diversity centers and postglacial colonization.]]>91115124Liston, A. and J.A. Wheeler, 1994Astragalus (Fabaceae) evidence from the chloroplast gene rpoC1 and rpoC2.]]>22377388Medica-Saric, M., I. Jasprica, A. Smolcic-Bubalo and A. Mornar,200477361366Mehrnia, M., S.H. Zarre and A. Sokhan Sanj,2005Astragalus microcephalus complex (Fabaceae), using RAPD.]]>33149158Mooney, H.A. and W.D. Billings,1959Oxyria digyna.]]>31129Perez-Alonso, M.J., A. Velasco, J. Paul and J. Sanz,2003Artemisia pedemontana gathered in spain.]]>317784Rejmanek, M.,199678171181Sefidkon, F., R. Kalvandi, M. Atri and M.M. Barazandeh,2003Thymus eriocalyx chemotypes.]]>2003Sefidkon, F., R. Kalvandi, M. Atri and M.M. Barazandeh,2005Thymus eriocalyx (Ronninger) Jalas.]]>20521524Semmar, N., M. Jay, M. Farman and R. Chemli,2005Astragalus caprinus (Fabaceae) based on the flavonic patterns.]]>33187200Telascrea, M., C.C. Araujo, M.O.M. Marques, R. Facanali, P.L.R. Moraes and A.J. Cavalheiro,2007Cryptocarya mandioccana Meisner (Lauraceae): Composition and intraspecific chemical variability.]]>35222232Turesson, G.,19223100113Zarre, S.H. and Podlech,1997Astragalus.]]>4243250