Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.1918.1921Listeria monocytogenes, Escherichia coli O157:H7 and Pseudomonas aeruginosa on Food Soiled Plastic Surfaces]]>TrachooN. 1120071011This study investigated the ability of three different pathogens, Escherichia coli O157:H7, Listeria monocytogenes and Pseudomonas aeruginosa, to attach food soiled (salad dressing, cooking oil, milk and yogurt) polyvinyl chloride (PVC) plastic. The pre-soiled PVC coupons were incubated with 108 CFU/cm2 of each bacterium in 50% tryptic soy broth for 6 h to allow attachment. It was found that the effect of food products was not significant while the effect of bacterial strain was highly significant (p = 0.0005). Mean attachment of P. aeruginosa (5.5 log CFU/cm2) to pre-soiled coupons was the highest (p<0.05), compared to L. monocytogenes (5.0 log CFU/cm2) and E. coli O157:H7 (4.5 log CFU/cm2). This study indicates the capability of each bacterial pathogen, E. coli O157:H7, L. monocytogenes or P. aeruginosa, to equally attach to different pre-soiled PVC surfaces. P. aeruginosa showed the greatest potential to attach to PVC when compared to E. coli O157:H7 and L. monocytogenes.]]>Chmielewski, R.A.N. and J.F. Frank,200322232Costerton, J.W., Z. Lewandowski, D.E. Caldwell, D.R. Korber and H.M. Lappin-Scott,199549711745Cowell, B.A., M.D.P. Willcox, B. Herbert and R.P. Schneider,199986944954De Beer, D., R. Srinivasan and P.S. Stewart,19946043394344Fatemi, P. and J.F. Frank,199962761765Frank, J.F. and R. Chmielewski,20016411781182Korber, D.R., G.A. James and J.W. Costerton,19946016631669Lebert, I., S. Leroy and R. Talon,200727281287Mosteller, T.M. and J.R. Bishop,1993563441Ronner, A.B. and A.C.L. Wong,199356750758Somers, E. and A. Wong,20046722182229Trachoo, N.,200325807815Trachoo, N., J.F. Frank and N.J. Stern,20026511101116Trachoo, N. and J.D. Brooks,20058599605Wirtanen, G., S. Salo, I.M. Helander and T. Mattila-Sandholm,2001Pseudomonas biofilm.]]>203750