Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.1562.1570Salmonella enteritidis in Poultry Samples by PCR, Plasmid Curing, Antibiotic Resistance and Protein Pattern Analysis]]>MirmomeniM. H.ColagarA. HosseinzadehGhazaeyS. 1020071010In this study two control isolates of Salmonella enteritidis, RTCC1623 and RTCC1624, were obtained from the institute of Razi (Karaj-Iran) and 14 strains were isolated from poultry samples in Kermanshah province of Iran, according to a standard protocol. These isolates were confirmed by PCR amplification of SefA gene fragments. Results showed that, 6 isolates of 14 isolates of Salmonella which their biochemical tests were positive contain 511 bp amplified fragments of the SefA gene. In other purpose, to correlating the presence of plasmids with antibiotic resistance and protein pattern, plasmid DNA was isolated before and after plasmid curing by using the alkaline lysis method. Strains of S. enteritidis contain seven different plasmid profiles (P1-P7) which were characterized by antibiotic resistance and protein pattern. Our observed showed, there was a high molecular weight plasmid with Rf 0.17 in all strains and the frequency of other plasmids was low. The plasmid with Rf about 0.2 is responsible for resistance to Cephalothin and the isolates that lost it were susceptible to this antibiotic. All strains, 100%, were resistant to ampicillin before and after curing of strains. According to present findings, PCR is a rapid and sensitive method for typing of S. enteritidis and plasmid profiling; antibiotic resistance and protein pattern are suitable methods for subtyping of S. enteritidis isolates. No direct correlation was found between plasmid contents, antibiotic resistance patterns and protein profiles of local S. enteritidis isolates.]]>Baulmer, A.J., B.M. Hargis and R.M. Tsolis,2000Salmonella outbreaks.]]>2875052Bhattacharya, S.K. and A.K. Dudey,1995Escherichia coli over expressing target gene.]]>1711551160Bichler, L.A., K.V. Nagaraja and B.S. Pomeroy,1994Salmonella enteritidis of animal, poultry and human origin.]]>57411Brown, D.J., D.L. Baggesen, H.C. Hansen and M. Bisgaard,1994Salmonella enterica serovar enteritidis by phage typing and plasmid profiling.]]>102208214Brown, T.A.,1999Buisan, M., J.M. Rodriguez-Pena and R. Rotger,1994Salmonella enteritidis virulence plasmid and its homology with the plasmid of Salmonella typhimurium.]]>16165169Candrian, U.,19952389103Christensen, P., E. Olsen, C. Hansen and M. Bisgaard,1992Salmonella enterica serovar gallinarum and pullorum by plasmid profiling and biochemical analysis.]]>21461470Corpus, L.O., A.M. Martinez, J.V. Navarrete and V.R.T. Gutirerrez,200442325332Dorn, C.R., R. Silapanuntakul, E.J. Angrick and L.D. Shipman,1993Salmonella enteritidis isolated from human gastroenteritis cases and from epidemiologically associated poultry flocks.]]>111239243Fernandes, S.A., C.R.T. Ghilardi, M.O. Tavechio Machado and C.C. Pignatari,2003Salmonella enteritidis strains isolated in Sao Paulo, Brazil.]]>455963Fluit, A.C., M.N. Widjojoatmodjo, A.T.A. Box, R. Torensma and J. Verhoef,1993Salmonella in poultry with the magnetic immuno-polymerasechain reaction.]]>5913421346Gado, I., V.G. Laszlo, B. Nagy, H. Milch, I. Drin, M. Awad-Masalmeh and J. Horvath,1998Salmonella enteritidis.]]>287509519Gast, R.K. and C.W. Beard,1990Salmonella enteritidis-contaminated eggs by experimentally infected hens.]]>34438446Gazouli, M., E. Tzelepi, A. Narkogiannakis, N.J. Legakis and L.S. Tzouvelekis,1998Salmonella typhimurium.]]>165289293Gupta, R., P. Sharma and V.V. Vyas,1995Escherichia coli.]]>412937Helmuth, R., R. Stephan, C. Bunge, B. Hoog, A. Steinbeck and E. Bulling,1985Salmonella serotypes.]]>48175182Holmes, B. and H.M. Aucken,1998Citrobacter, Entrobacter, Klebsiella and Other Members of the Enterobacteriaceae.]]>1998pp: 999-1033pp: 999-1033Humphrey, T.J., A. Baskerville, H. Chart, B. Rowe and A. Whitehead,1991Salmonella enteritidis PT4 infection in specific pathogen free hens: Influence of infecting dose.]]>1482485Icgen, B., G.C. Gurakan and G. Úzcengiz,2001Salmonella isolates.]]>18631635Keller, L.H., D.M. Schifferli, C.E. Benson, S. Aslam and R.J. Eckroade,1997Salmonella enteritidis.]]>41535539Kid, H., D.H. Read, R.P. Chin, A.A. Bickford, R.L. Walker and A. Ardans,1996Salmonella enteritidis phage type 4 infection in a commercial layer flock in Southern California. Bacteriologic and epidemiologic finding.]]>40665671Kisiela, D., M. Kuzkowski, A. Wieliczko, I. Sambor-Mazurkiewicz and M. Ugorski,2002SefA, FimA and AgfA fimbrial roteins of S. enteritidis in their abilities to elicit humoral immune response in hens.]]>4795105Laemmli, U.K.,1970227680685Lee, L.A., N.D. Puhr, E.K. Maloney, N.H. Bean and R.V. Tauxe,1994170128134Mansi, M., K.J. anderson, C.A Inche, L.K. Knowles and D.J. Platt,2000Klebsiella pneumoniae large indigenous plasmid using sodium dodecyl sulphate.]]>151201208Mead, P.S., L. Slutsker, V. Dietz, L.F. McCaig and J.S. Bresee et al.,19995607625Millemann, Y., M.C. Lesage, E.C. Dancla and J.P. Lafont,1995200 for tracing avian isolates of Salmonella typhimurium and S. enteritidis.]]>33173179Olsen, J.E., D.J. Brown, M.N. Skov and J.P. Christensen,199315125135Parry, M.F.,198977694Pohl, P., P. Lintermans, M. Marin and M. Couturier,1991Salmonella enteritidis strains of animal origin in Belgium.]]>1061116Poppe, C., W.D. McFadden and R.P. Jhonson,1993Salmonella enteritidis phenotypes 4, 8 y 13 and other Salmonella spp. for dag-old chichs, hens and mice.]]>57281287Poppe, C., K.A. McFadden, A.M. Brouwer and W. Demezuk,1993S. enteritidis strains.]]>57176184Rychlik, I., F. Sisak and P. Lany,1993Salmonella enteritidis and Salmonella typhimurium by plasmid profile analysis and restriction endonuclease analysis of chromosomal DNA.]]>38433439Sambrook, J., E.F. Gritsch and T. Maniatis,20013rd Edn., Vol. 3,pp: 444-453pp: 444-453Schrank, I.S., M.A.Z. Mores, J.L.A. Costa, A.P.G. Frazzon and R. Sonicini et al.,2001Salmonella in poultry industry products and clinical samples.]]>824553Sherratt, D.J., L.K. Arciszewska, G. Bladely, G. Colloms, S. Grant, K. Leslie and R. McCulloch,19953473742Shivaprasad, H.L., J.F. Timoney, S. Morales, B. Lucio and R.C. Baker,1990Salmonella enteritidis infection in laying chickens. I. Studies on egg transmission, clinical signs, fecal shedding and serologic responses.]]>34548557Stone, G.G., R.D. Oberst, M.P. Hays, S. McVey and M.M. Chengappa,1994Salmonella serovars from clinical samples by enrichment broth cultivation-PCR procedure.]]>3217421749Summers, D.K.,19919273278Summers, D.K., C.W. Benton and H.L. Withers,1993810311038Tan, R.L., C.W. Koons, B. Barrett, R.F. Teclaw and D. Bixler et al.,1999Salmonella enteritidis in Humans and Animals Changes in Phenotypic and Genotypic Characteristics in Ten Years.]]>Tassios, T., A. Markkogiannakis, C. Vatopoulos, E. Katsanikou, N. Velonakis, J. Kremanstinou and J. Legakis,1997Salmonella enteritidis during a 7 year Period in Greece.]]>3513161321Threlfall, E.J., B. Rowe and L.R. Ward,1989Salmonella enteritidis phage types by plasmid profile typing.]]>102459465Tomas, J.M., R. Pares, J.M. Somers and W.W. Kay,1986353742Towner, K.J. and A. Cockayne,1993pp: 28-63pp: 28-63Tsai, C.M. and C.E. Frasch,1982119115119Tsen, H.Y. and J.S. Lin,2001S. enteritidis strains isolated form food-poisoning cases in Taiwan by pulsed field gel electrophoresis, plasmid profile and phage typing.]]>917279Turcotte, C. and M.J. Woodward,1993S. enteritidis.]]>3914771485Vatopoulos, A.C., E. Mainas, E. Balis, E.J. Threlfall and M. Kanelopoulou et al.,1994S. enteritidis.]]>3213221325Viret, J.F., A. Bravo and J.C. Alonso,199155675683Ward, L.R., J.D.H. De-Sa and B. Rowe,1987Salmonella enteritidis.]]>99291294Wegener, H.C., D.L. Baggesen and P. Gerner-Smidt,1997Salmonella and salmonellosis in Denmark.]]>1997pp: 551554