Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.1481.1485Bacillus sp. As Probiotic Bacteria on Growth of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man)]]>DeeseenthumSirirat LeelavatcharamasVichai D. BrooksJohn 92007109 The effects of feeding two Bacillus spp. isolated from the intestine of the giant freshwater prawn on the growth of Giant Freshwater Prawns (Macrobrachium rosenbergii de Man) was examined. The isolated Bacillus KKU02 and Bacillus KKU03 (~107 CFU mL-1) were mixed into commercial prawn feed (200 mL kg-1). After rearing shrimp with the bacteria in four feed treatments, (Bacillus KKU03, Bacillus KKU02, mixed culture and control groups) for 120 days, body length and weight of the prawns in mixed culture tanks were significantly higher (p = 0.05) than in the control tanks (7.48 cm and 3.32 g, vs 6.6 cm and 2.1 g, respectively). Both isolates were found to produce amylase and protease. The stabilities of the single Bacillus sp., mixed culture and commercial probiotic in the feeds were examined during storage at 4°C and room temperature. The percentage viability of Bacillus KKU02, Bacillus KKU03 and mixed culture stored at room temperature declined dramatically to 2.54, 21.88 and 10.92% within 2 weeks, respectively. At 4°C however, the percentage viability of the tested probiotics reduced slowly. The survival of the commercial probiotics was the same at both temperatures about 50% after 70 days storage.]]>Amabile-Cuevas, C.F., M. Gardenas-Garcia and M. Ludgar,199583320329Armstrong, D.A., M.J. Stephenson and A.W. Knight,1976Macrobrachium rosenbergii.]]>93946Austin, B., L.F. Stuckey, P.A.W. Robertson, I. Effendi and D.R.W. Griffith, 1995Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii.]]>189396Bly, J.E., S.M.A. Quiniou, L.A. Lawson and L.W. Clem,1997Pseudomonas fluorescens.]]>203540Gatesoupe, F.J.,1991Brachionus plicatilis and their dietary value for larval turbot, Scophthalmus maximus.]]>96335342Graam, L., J. Melchiorsen, B. Spanggaard, I. Huber and T. F. Nielsen,1999Vibrio anguillarrum by Pseudomonas fluorescens AH2, a possible probiotic treatment of fish.]]>65969973Hsieh, C.H., N.H. Chao, L.A.O. Gomes and I.C. Liao,1989Macrobrachium Rosenbergii, in Taiwan.]]>1989pp: 85-109pp: 85-109Munilla-Moran, R., J.R. Stark and A. Barbour,1990Scophthalmus maximus L.).]]>88337350New, M.B.,19908899143Robertson, P.A.W., C. O`Dowd, C. Burrells, P. Williams and B. Austin,2000Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum).]]>185235243Saxena, V., 200381718Smith, P. and S. Davey,1993Aeromonas salmonicida from fish with stress-inducible furunculosis by a fluorescent pseudomonad.]]>16521524Devaraja, T.N., F.M. Yusoff and M. Shariff,2002206245256Westerdahl, A., J.C. Olsson, S. Kjelleberg, P.L. Conway,1991Scophtalmus maximus)-associated bacteria with inhibitory effects against Vibrio anguillarum.]]>5722232228Gatesoupe, F.J.,1994Scophthalmus maximus, against pathogenic Vibrio sp.]]>7277282