Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.514.518in vitro Clonal Propagation of Lavendula officinalis chaix a Multipurpose Plant of Industrial Importance]]>ChishtiNahida KalooZ. A.ShawlA. S.SultanPhalisteen 3200693An efficient regeneration protocol has been developed for rapid and mass scale clonal propagation of Lavendula officinalis from shoot tip explants. For the induction of multiple shoot formation, the explants were cultured on Murashige and Skoog`s (MS) basal medium supplemented with different concentrations of cytokinins viz., BAP (0.5-2.0 mg L-1), Kn (0.5-2.0 mg L-1) and on different cytokinin plus auxin combinations viz., BAP (1.0-2.0 mg L-1+IAA (0.5-1.0 mg L-1) and Kn (1.0-2.0 mg L-1) + IAA(0.5-1.0 mg L-1). These shoot tips produced multiple shoots, with the best result in BAP (2.0mg L-1) whereby (45±6.40) shoots were produced/explant in 80% cultures in 4 weeks time. The regenerated shoots were subcultured on the best induction medium (BAP 2.0 mg L-1) after every 4 weeks so as to increase their number. These shoots rooted best in half strength MS basal medium fortified with IBA (1.0mg L-1) with 7-18 roots per shoot(Avg. 16±6.89) roots in 80% cultures in 4 weeks time. Rooted plantlets were shifted to the plastic pots containing mixture of sand:clay:vermiculite (1:1:1) and kept for two weeks under controlled conditions of temperature (25±2°C) and relative humidity 60%. Afterwards these plantlets were hardened in green house environment (Vista Biocell Limited ) for one month and finally shifted to field conditions were 70% plants survived.]]>Andrade, L.B., S. Echeverrigaray, F. Fraearo, J.F. Pauletti and L. Rota,1999Lavendula vera DC).]]>567983Anonymous,199983102102Banthrope, D.V., A.B. Stephanie, N. Vincent Co, M.G. Osborne and D.G. Watson,200125629636Carlos, M., S. Gras and M. del Carmen Calvo,1996Lavendula latifolia through nodal bud culture of mature plants.]]>45259261Dias, M.C., R. Almeida and A. Romano,2002Lavendula viridis L. Her through in vitro axillary shoot proliferation.]]>6899102Dronne, S., F. Jullien, J.C. Caissard and O. Faure,1999in vitro shoot regeneration from leaves of Lavendin (Lavendula xxintermedia Emerck exloiseleur).]]>18429433Gattefosse, R.M.,1937Grieve, M.A.,1931Gimpsey, J.A. and N.G. Porter,1999Murashige, T. and F. Skoog,196215473497Nakahma, H., K. Sonomoto, F. Sato, Y. Yamada and A. Tanaka,1990Lavendula vera and characterization of a component of the pigments.]]>545359Segura, J. and M.C. Calvo,1991Lavendula spp. (Lavender): In vitro Culture, Regeneration of Plants and the Formation of Essential Oil and Pigments.]]>1991pp: 283-310pp: 283-310Shawl, A.S. and S. Kumar,2000Lavender oil industry in Kashmir.]]>22319321Tajjudin, A.S. Shawl, S. Kumar, M.C. Nigam and A. Hussain,1983275659Tsuro, M., M. Inoue and H. Kameoka,2001Lavedula vera DC).]]>88309317Tsuro, M., M. Koda and M. Inoue,1999Lavendula vera DC).]]>81331336Webb, J.K., D.V. Banthrope and D.G. Watson,198423903904