Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.2729.2733Lycopersicon esculentum L.)]]>ShahriariFarajollah HashemiHaleh HosseiniBahman 122006915In this study the effect of three genotypes (KalG, Kal-early and Su2270 as the commercial cultivars in Iran), two explants (cotyledon and hypocotyl), growth regulators (BAP, NAA and Zeatin) and a strain of Agrobacterium tumefaciense (pGV3850) on regeneration and transformation efficiency is reported. Although all cultivars and explants were regenerated on the different media, however our results showed that there were significant differences between genotypes and explants in the number of shoot formation per explant. Optimal regeneration of KalG, Kal-early and Su2270 cultivars were observed on MS media supplemented with 2 mg L-1 Zeatin and 2 mg L-1 BAP, respectively. The maximum number of shoot formation per explant was observed in cotyledon and hypocotyle explants in KalG and Su2270 cultivars, respectively. The transformation rate was 17% for Kal-early to 35% for KalG cultivars. Transformation were confirmed by Gus assay and PCR analysis.]]>Agharbaoui, Z., A.F. Greer and Z. Tabaeizadeh,1995Lycopersicon chilense Dun. by Agrobacterium tumefaciens.]]>15102105Arillage, R., C. Mascarell, E. Gisbert and V. Moren,1998HAL 2 gene in tomato increase the in vtiro salt tolerance of transgenic progenesies.]]>139219229Chi, Y.S. and G.C. Philips,1987Agrobacterium-mediated transformation of Lycopersicon based on conditions favorable for regeneration.]]>19105108Costa, M.G.C., F.T.S. Nogueira, M.L. Figueira, W.C. Otoni, S.H. Brommonschenkel and P.R. Cecon,2000Lycopersicon esculentum Mill.) cultivars.]]>19327332Delannay, X., B.J. LaVallee, R.K. Proksch, R.L. Fuchs and S.R. Sims et al.,1989Bacillus thuringiensis var. kurstaki insect control protein.]]>712651269Drorri, E. and A. Altman,200149152153Fillatti, J.J., J. Kiser, R. Rose and L. Comai,1987Agrobacterium tumefaciens vector.]]>5726730Fischhoff, D.A., K.S. Bowdish, F.J. Perlak, P.G. Marrone and S.M. McCormick et al.,19875807813Frary, A. and E.D. Earle,1996Agrobacterium-mediated transformation of tomato.]]>16235240Hamza, S. and Y. Chupeau,1993Agrobacterium-mediated transformation from tomato (Lycopersicon esculentum).]]>4418371845Hu, W. and G.C. Phillips,2001Agrobacterium tumefaciens-mediated transformation efficiency for cultivated tomato (L. esculentum).]]>371218Jefferson, R.A.,19875387405Ling, H.Q., D. Kriseleit and M.W. Ganal,1998Agerobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.).]]>17843847Lino, J.E., F. Rogerio, T. Agusto, F. Antonio and E.P. Lazaro,2004in vitro shoot regeneration.]]>167753757McCormick, S., J. Niedermeyer, J. Fry, A. Barnason, R. Horsch and R. Fraley,1986L. esculentum) using Agrobacterium tumefaciens.]]>58184Murashige, T. and F. Skoog,196215473497Oktem, H.A., Y. Bulbul, E. Oktem and M. Yucel,1999Agrobacterium-mediated transformation studies in tomato (Lycopersicon esculentum Miller).]]>23345348Park, S.H., J.L. Morris, J.E. Park, K.D. Hirschi and R.H. Smith,2003Agrobacterium-mediated tomato transformation.]]>16012531257Pozueta-Romero, J., G. Houlne, L. Canas, R. Schantz and J. Chamarro,2001Agrobacterium-mediated transformation.]]>67173180Roset, S. and J.W. Gilissen,198938123Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen and R.W. Allard,19848180148018Soniya, E.V., N.S. Banerjee and M.R. Das,20018012131215Tabaeizadeh, Z., Z. Agharbaoui, H. Harrak and V. Poysa,1999Lycopersicon chilense chitinase gene demonstrate improved resistance to Verticillium dahliae race 2.]]>19197202Van Roekel, J.S.C., B. Damm, L.S. Melchers and A. Hoekema,1993Lycopersicon esculentum).]]>12644647Velcheva, M., Z. Faltin, M. Flaishman, Y. Eshdat and A. Perl,2005Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum L. Mill.).]]>168121130Vidya, C.S.S., M. Manoharan, C.T.R. Kumar, H.S. Savithri and G.L. Sita,2000Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum var. pusa ruby) with coat-protein gene of Physalis mottle tymovirus.]]>156106110