Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.2369.2374in vitro Maturation]]>UllahIkram JalaliSamina KhanHamayun ShamiSajjad A.KiyaniMuhammad Mohsin122006912The present study was conducted to investigate the effect of L-ascorbic acid (AsA) on Nile Ravi buffalo oocytes. Cumulus Enclosed Oocytes (CEOs) and Cumulus Denuded Oocytes (CDOs) were cultured for 24 h in TCM-199 supplemented with Recombinant Human Follicular Stimulating Hormone (RhFSH), 0.4% Bovine Serum Albumin (BSA), oestradiol and Gentamycin. Nili Ravi buffalo oocytes with or free from cumulus cells were exposed to 250 μM AsA. Meiotic maturation, degeneration of oocytes was assessed at different maturation time (0, 12, 24 h). CDOs treated with L-ascorbic acid resumed meiosis and oocytes were significantly (p<0.05) increased to MII stage. Where as CDOs matured in control medium with out L-ascorbic acid supplementation did not show any significant increase in MII oocytes proportion as the maturation time was extended to 24 h (p<0.05). In present study it was observed that cumulus cells are involved in protection against degeneration as CEOs treated in control medium the degenerated oocytes high significantly (p<0.05) decreased to 7.40% as compared to CDOs in which 21.4% oocytes were found to be degenerated. These results indicate that exposure to L-ascorbic acid promote the development of the Nili Ravi buffalo CDOs and cumulus cells are involved in protection against degeneration of oocytes.]]>Arends, M.J. and A.H. Wyllie, 199132223254Byrd, J.A., S.L. Pardue and B.M. Hargis, 1993in vitro.]]>102279281Carolan, C., P. Lonergan, A. Van Langendonckt and P. Mermillod, 1995in vitro. ]]>4311151128Cetica, P.D., L.N. Pintos, G.C. Dalvit and M.T. Beconi, 2001in vitro maturation.]]>515764Christ, H.H., K. Joachim, L. Monika, G. 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