Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.1430.1437Trichoderma sp. FETL c3-2viaSolid State Fermentation Grown on Sugar Cane Baggase: Palm Kernel Cake as Substrates]]>KhengPang PeiIbrahimDarah PoppeLaszlo SzackacsGeorge OmarIbrahim Che8200698The production of cellulase by a local isolate Trichoderma sp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrate mixture was investigated. The optimized Solid State Fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake, moisture content of 75% (w/w), pH of moistening agent of pH 7.0, at 30°C and inoculum size of 1 x 108 spores mL-1. The SSF system was also supplemented with 4% (w/w) dextrin and 6% (w/w) of yeast extract as additional carbon and nitrogen sources, respectively. Cellulose at the concentration of 0.2% (w/w) was found to be a significant inducer for cellulase production. Using the optimized SSF system, maximum FPase and CMCase production of 3.3 U g-1 substrate and 18.05 U g-1 substrate, respectively, after 4 days of fermentation time at 30°C were obtained. The modification of the SSF system resulted a 105% increment of FPase and 63% of CMCase production. Although no significant increase in growth after modification was observed, the results obtained indicated that the cellulase production by Trichoderma sp. FETL c3-2 is growth dependent.]]>Adsul, M.G., J.E. Ghule, R. Singh, H. Shaikh, K.B. Bastawde, D.V. Gokhale and A.J. Varma,2004576772AOAC,199716th Edn.,pp: 25-28pp: 25-28Gessesse, A. and G. Mamo,1999Bacillus sp. by using solid state fermentation.]]>256872Ghose, T.K.,198759257268Pau, H.S. and I.C. Omar,2004Aspergillus flavus USM A10 via solid state fermentation (SSF) on rice husks and wood dusts as substrates.]]>712491256How, S.P., A.N. Ooi and C.O. Ibrahim,2004spergillus terreus E5 on rubberwood saw dusts as substrate.]]>335360Ikasari, L. and D.A. Mitchell,1994Rhizopus oligoporus in solid state fermentation.]]>10320324Jecu, L.,20001115Kang, S.W., Y.S. Park, J.S. Lee, S.I. Hong and S.W. Kim,2004Aspergillus niger KK2 from lignocellulosic biomass.]]>91153156Ogel, Z.B., K. Yarangumeli, H. Dundar and I. Ifrij,2001Schtalidium thermophilum on complex lignocellulosic biomass for endoglucanase production.]]>28689695Panagiotou, G., D. Kekos, B.J. Macris and P. Christakopoulos,2003Fusarium oxysporum grown on corn stover in solid state fermentation.]]>183745Pandey, A.,1992271217Kheng, P.P. and I.C. Omar,2005Aspergillus niger USM AI 1 via solid state fermentation using palm kernel cake (PKC) as substrate.]]>27325336Raimbault, M. and D. Alazar,19809199209Smith, P.J., A. Rinzema, J. Tramper, E.E. Schlosser and W. Knol,199631669678Solis-Pereira, S., E. Favela-Torres, G. Viniegra-Gonzalez and M. Gutierrez-Rojas,1993Aspergillus niger in submerged and solid state fermentations.]]>393641Sternberg, D.,1976Trichoderma.]]>63553Sudgen, C. and M.K. Bhat,1994Sporotrichum thermophile.]]>10444451Swift, J.,19725321322Szcwczyk, K.W. and L. Myszka,1994Aspergillus niger in solid state fermentation.]]>10123126Virupakshi, S., K. Giresh Babu, S.R. Gaikwad and G.R. Naik,2005Bacillus sp. JB-99 in solid state fermentation.]]>40431435Wen, Z., W. Liao and S. Chen,2005Trichoderma reesei from dairy manure.]]>96491499