Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2003.1250.1252in vitro Anther Culture]]>ShahnewazSharmin BariM. A.SiddiqueN. A.KhatunN. RahmanM. H.HaqueM. E.122003614Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinin). The best callusing from cultured anthers obtained in 2 medium containing 2,4-D 2 mg l-1, NAA 2.5 mg l-1 and kin 0.5 mg l-1. For regeneration of haploid plantlets anther derived calli were transferred to modified MS medium enriched with kin 1 mg l-1, NAA 1 mg l-1, BAP 1.0 ml l-1 and incubated at 25±1OC in light. BRRI Dhan-29 produced both green and albino plants while BR-3 produced green plants only. Green plantlets obtained from BRRI Dhan-29 were examined and found to be 69.2% haploid plants.]]>Bourgin, J.P. and J.P. Nitsch,1967in vitro.]]>9377382Cao, M.Q., F. Li, T. Jiang and G.S. Lin,19953922728Challef, R.S. and A. Stolarz,1981Oryza) anthers.]]>51210216Chen, C.C. and M.H. Lin,1976171829Chen, C.C.,1977In vitro Development of plants from microspore of rice.]]>13484489Chen, C.C., H.S. Tsay and C.R. Huang,1991Oryza sativa L.).]]>1991pp: 192-215pp: 192-215Chu, C.C.,19821982Collins, G.P.,197717583586Hakim, L., A.J. Miah and M.A. Mansur,1991In vitro plant regeneration in rice through anther culture.]]>18589Han, H.,1985pp: 75-84pp: 75-84Huang, H.S., T.H. Ling, P.L. Teseng, Y.L. Shin and P. Shi,1978Oryza sativa L.) Subsphsien by methods of mathematical analysis.]]>1978pp: 244246Kasha, K.J.,19741974pp: 67-87pp: 67-87Murashige, T. and F. Skoog,196215473497Niizeki, H. and K. Oono,196844554557Oono, K.,1975260139222Raina, S.K.,199716141186Raina, S.K. and F.J. Zapata,1997116305315Rush, M.C.,19811981Snape, J.W.,1981Sunderland, N.,19741974pp: 91-122pp: 91-122Yin, K.C., S.T. Hsu, C.Y. Chu, F.Y. Pi and S.T. Wang et al.,197619227242Zapata, F.J., J.P. Crill, T.W. Mew, R.O. Romoro, L.R. Torrizo, H.S. Aleigjar and G.S. Kush,1983pp: 27-49pp: 27-49Guha, S. and S.C. Maheshwari,1964In vitro production of embryos from anthers of Datura.]]>204497497