Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2002.956.958Zea mays Var. EV-2097]]>NaqviS.M. S.YasminT. RashidH. ChaudaryZ. QuraishiA. 9200259The present study was conducted on maize, with the aim to work out a suitable surface disinfecting procedure for seeds and to know the best concentration of 2,4-dichlorophenoxyacetic acid (2,4 -D) for callus induction in N6 medium using var. EV-2097. Better disinfection of seeds was achieved with 30 minutes Clorox treatment (86 %). Maximum callus induction (47 %) was recorded with 2 mg l-1 followed by 3 mg l-1 (18 %) and 1 mg l-1 2,4 -D (16 %).]]>Anonymous,2000pp: 13pp: 13Ashton, F.M. and A.S. Crafts, 1981pp: 166-175pp: 166-175Chu, C.C.,19781978pp: 4550Chu, C.C., C.C. Wang, C.S. Sun, K.C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi,197518659668Close, K.R. and L.A. Ludeman,1987528189Jugenheimer, R.W.,19761st Edn.,pp: 16pp: 16Leonard, W.H. and J.H. Martin,19631st Edn.,pp: 131pp: 131Li, H., F. Li, H. Gui-Rue, W. An and H.F. Li et al.,1999221012Lowe, K., D.B. Taylor, P. Ryan and K.E. Peterson,198541125132Naqvi, S.M.S., S.T. Abbas and A. Quraishi,198910224230Narayanaswamy, S.,19901990pp: 185pp: 185Pareddy, D.R. and J.F. Petolino,199067211219Piralov, G.R. and O.E. Abraimova,1999732829Russell, A.D., W.B. Hugo and G.A.J. Ayliffe,19922nd Edn.,Sawahel, W.A. and A.M. Ali,1994Zea mays Kernel.]]>16397400Straus, J. and C.D. Larue,1954in vitro I. Culture requirements.]]>41687694Walden, D.B.,19781st Edn.,pp: 8pp: 8Wernike, W., J. Gorst and L. Milkovits,198668597602Yamada, Y.,19901990pp: 144pp: 144