Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2002.75.79Rauvolfia serpentina by Tissue Culture Technique]]>AhmadShahrear AminM. N. AzadM.A.K. MosaddikM. Ashik 1200251The shoots of Rauvolfia serpentina from the field growing plants were used as explants and cultured on MS medium supplemented with 2.5 mg/L BA + 0.1 mg/L NAA to afford multiple shoots. It was evident that MS + 0.5 mg/L BA + 0.2 mg/L NAA was the most suitable combination for induction of callus and development of shoot buds formation but it failed to support elongation of shoot. So, the cultures with tiny shoots was transferred to 2.0 mg/L BA with 0.05mg/L NAA, where they showed excellent elongation. Regenerated shoots rooted well 0.2 mg/L IBA+ 0.2mg/L NAA combination. After having acclimatization the regenerated plantlets were transferred to the open field where 95% of the plant-lets survived and showed excellent growth.]]>Hu, C.Y. and P.J. Wang,19831983pp: 177-227pp: 177-227Kirtikar, K.R. and B.D. Basu,1993Vol. 2,pp: 289pp: 289Mathur, A., P.S. Ahuza and A.K. Mathur,1993Panax Quinquefolium, R. Serpentina and Some Other Medicinal and Aromatic Plants of India.]]>1993pp: 155-173pp: 155-173Mathur, A., A.K. Mathur, A.K. Kukreja, P.S. Ahuja and B.R. Tyagi,1987R. serpentina L. Benth ex Kurz. through tissue culture.]]>10129134Roy, S.K., M.Z. Hossain and M.S. Islam,1994in vitro shoot tip culture.]]>46975Sarkar, K.P., A. Islam, R. Islam, A. Hoque and O.I. Joarder,1996Rauwolfia serpentina by organogenesis from callus cultures.]]>66365Ghani, A.,19982nd Edn.,pp: 276pp: 276