Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2002.589.593Aspergillus fumigatus]]>GoudaMona K.5200255A local isolate identified as Aspergillus fumigatus was tested for inulinase and invertase production. Different carbon sources alone or in combination with inulin were tested. About 91% inulinase was found as extracellular enzyme in culture medium with inulin and wheat bran as sole C-source (1% for each). The activity towards sucrose (invertase enzyme) was detected only in the supernatant and represents about 26% from the inulinase activity. The production of both enzymes was inhibited with ammonical compounds as nitrogen source, whereas the maximum level (3.72, 0.35 U/ml for inulinase and invertase respectively) was observed when 0.38% KNO3 was used as nitrogen source. Beef extract, yeast extract, peptone and tryptone reduced the amount of inulinase by about 83-91%. Both enzymes were produced in a broad pH range between 4.0-8.0 as initial pH. Optimum temperature was found to be 30°C for inulinase and a dramatic reduction was observed above 35°C, while 40°C was observed for invertase. The properties of crude inulinase were also studied. The enzyme has optimum pH 5.5 in acetate buffer and optimum temperature 45°C. Fructose at concentrations higher than 0.2mg/reaction mixture inhibited the enzyme activity. The enzyme was stable for 30 min at 30°C and 40°C in pH 5.5 and for 24h at 4°C in pH 6 - 6.5. Addition of 10% glycerol protected the enzyme from deactivation at 45°C and 50°C for 30 min. The enzyme was completely inhibited by 1mM Ag+ and Hg2+ and activated by the presence of 1mM Ca2+ or Mn2+.]]>Bradford, M.M.,197672248254Derycke, D.G. and E.J. Vandamme,1984Aspergillus niger inulinase.]]>344551Efstathiou, I., G. Reysset and N. Truffaut,1986Clostridium acetobutylicum Strain AKBn8.]]>25143149Ettalibi, M. and J.C. Baratti,1990Aspergillus ficuum inulinases.]]>546168Gupta, A.K., P. Rathore, N. Kaur and R. Sing,1990Fusarium oxysporum.]]>47245257Miller, G.L.,195931426428Vandamme, E.J. and D.G. Derycke,198329139176Vullo, D.L., C.E. Coto and F. Sineriz,1991Bacillus subtilis 430 A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby).]]>5723922394Xiao, R., M. Tanida and S. Takao,1988Chrysosporium pannorum.]]>65553558Yokota, A., S. Hirayama, K. Enomoto, Y. Miura, S. Takao and F. Tomita,1991Artrobacter sp. H 65-7 and preparation of DFA III from Inulin by the enzyme.]]>72258261Pandey, A., C.R. Soccol, P. Selvakumar, V.T. Soccol, N. Krieger and J.D. Fontana,1999813552Grootwassink, J.W.D. and S. Fleming,1980Kluyveromyces fragilis: Batch and continuous fermentation, simple recovery method and some industrial properties.]]>24553Kim, K.C.,197518177182Nakamura, T. and S. Nakatsu,1977Penicillium.]]>512329Nakamura, T., S. Hoashi and S. Nakatsu,1978Aspergillus.]]>52105110Negoro, H.,1978Kluyveromyces fragilis.]]>56102107Negoro, H. and E. Kito,1973Candida kefyer.]]>5196102Pandey, A., S. Joseph, L. Ashakumary, P. Selvakumar and C.R. Soccol,199982103114Rutherford, P.P. and A.C. Deacon,1972Taraxacum officinale Weber).]]>126569576Snyder, H.E. and H.J. Phaff,1960Saccharomyces fragilis. A. van leeuwenhoek.]]>26433452Toran-Diaz, I., V.K. Jain, J.J. Allais and J. Baratti,1985Zymomonas mobilis growing on Jerusalem artichoke juice.]]>7527530Zittan, L.,198133373377Nakamura, T., A. Shitara, S. Matsuda, T. Matsuo, M. Suiko and K. Ohta,1997Penicillium sp. TN-88 that liberates inulotriose.]]>84313318