Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2001.271.276E. W.Jwanny El-SayedS. T. A. M. Salem A. N. Shehata 3200143Chitinase, laminarinase A and laminarinase B were extracted and purified from
Egyptian sugar beet (Beta vulgaris) leaves. Chitinase showed higher heat
stability than laminarinase A and laminarinase B when heated at 50°C for 60 min.
Chitinase activity was quite stable in water than in buffer, while laminarinase
A and laminarinase B activities increased when incubated with 0.1M citrate-phosphate
buffer at pH 6.0 to 7.0 for 30 min. The Km values of chitinase, laminarinase
A and laminarinase B were 0.2, 0.27 and 0.074% at pH`s 4.5, 4.5 and 6.5 using
colloidal chitin, laminarin A and laminarin B as substrates respectively. Chitinase
enzyme was activated by 0.75 mM - mercaptoethanol by 1.6 times, while laminarinase
A enzyme was activated by 1.0 mM of CuCl2, FeSO4 and EDTA
with 2.35, 1.38 and 1.57 times respectively. Laminarinase B enzyme was activated
by 1.0 mM Zn SO4 and K2SO4 with 1.3 and 1.18
times respectively. Chitinase, laminarinase A and laminarinase B enzymes have
an endo-splitting type of activity. They were able to inhibit the growth and to
lyse cell walls of Aspergillus oryza and A. flavus either alone or in combination
through the degradation of chitin and laminarin. ]]>Ariolo, M., A. Ludwig, T. Boller and P. Bonfante,19921713443Bartnicki-Garcia, S.,19682287108Benhamou, N., K. Broglie and I. Chet,1993Rhizoctonia solani: Ultrastructural changes and cytochemical aspects of chitin breakdown.]]>39318328Benhamou, N., K. Broglie, I. Chet and R. Broglie,1993Rhizoctonia solani cytochemical aspects of chitin breakdown in vivo.]]>4295305Boller, T., A. Gehri, F. Mauch and U. Vogeli,19831572231Chang, C.T., Y.L. Hsueh and H.Y. Sung,199640417425Clarke, A.E. and B.A. Stone,1962Vitis vinifera) and other plants.]]>1175188Davis, B. and D.E. Eveleigh,19841984pp: 161-179pp: 161-179Deloire, A., E. Kraeva, M.C. Maura, E. Bonnet and J. Bierne,1997in vitro by embryogenic and non-embryogenic cells of grapevines.]]>365152El-Sayed, S.T.,199432155162El-Sayed, S.T., A.M. Salem, A.N. Shehata and E.W. Jwanny,2000Beta vulgaris and other higher plants.]]>3250256Han, B.K., W.J. Lee and D.H. Jo,19974095100Huotari, F.I., T.E. Nelson, F. Smith and S. Kirkwood,1968Basidiomycete species QM 806.]]>243952956Huynh, Q.K., J.R. Borgmeyer, C.E. Smith, L.D. Bell and D.M. Shah,1996Engelmannia pinnatifida.]]>316723727Keen, N.T. and M. Yoshikawa,198371460465Kisser, M., C.P. Kubicek and M. Rohr,1980Aspergillus niger during citric acid fermentation.]]>1282633Kono, M., T. Matsui, C. Shimizu and D. Koga,1990Anguilla japonica.]]>54973978Lineweaver, H. and D. Burk,193456658666Luchsinger, W.W., W.J. Ferrell and G.L. Schneberger,196340554563Mauch, F., B. Mauch-Mani and T. Boller, 198888936942Molano, J., I. Polacheck, A. Duran and E. Cabib,197925449014907Moore, A.E. and B.A. Stone,1972Nicotiana glutinosa: I. Extraction, purification and physical properties.]]>258238247Moore, A.E. and B.A. Stone,1972Nicotiana glutinosa II. Specificity, action pattern and inhibitor studies.]]>258248264Nielsen, K.K., J.D. Mikkelsen, K.M. Kragh and K. Bojsen,1993Cercospora beticola, characterization and expression in transgenic tobacco plants.]]>6495506Nelson, N.,1944153375380Park, R., K.S. Song and I. Jung,199235191195Porfireva, O., D. Yusupova, N. Zotkina, R. Sokolova and L. Gabdrakhmanova,1997Serratia marcescens and Peculiarities of its biosynthesis.]]>66347353Roberts, W.K. and C.P. Selitrennikoff,1986880161170Roberts, W.K. and C.P. Selitrennikoff,1988134169176Salem, A.M., S.T. El-Sayed, A.N. Shehata and E.W. Jwanny,199959932Smogyi, M.,19521951923Tsujisaka, Y., Y. Tominaga and M. Iwai,19733725172525Wadsworth, S.A. and J.P. Zikakis,19843212841288Wilson, C.M.,19593111991201Yoshida, S., S. Kasuga, N. Hayashi, T. Ushiroguchi, H. Matsuura and S. Nakagawa,198753615617Zhang, Y.Y., N.H. Haunerland and Z.K. Punja,1996Daucus carota) roots and purification and characterization of a novel isoform.]]>96130138