Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2001.266.270AngD. C. S. Abd-Aziz H. M. Yusof M. I. A. Karim A. Ariff K. Uchiyama S. Shioya 3200143Rice α-amylase and glucoamylase produced by S. cerevisiae YKU 107 and YKU
131, respectively were partial purified using anion exchange chromatography and
characterised. The optimum active conditions for -amylase was at pH 5.0 and temperature
of 50°C. It was capable in hydrolysing more than 85% of potato, rice, cassava
and sago starch, however, it could only hydrolysed 64% of corn starch. Glucoamylase
was optimally active at pH 5.5 and temperature of 55°C. Glucoamylase could hydrolyse
more than 70% of cassava, sago and potato starch, it also hydrolysed more than
65% of corn and rice starch. Both of the enzymes were not severely affected by
the changes of ionic strength of acetate buffer at molarity lower than 0.20 M.]]>Beggs, J.D.,1978275104109Bon, E.,1991Aspergillus awamori.]]>1991Coombs, J.,19841311345De Moraes, L.M.P., S. Astolfi-filho and S.G. Oliver,19954310671076Hartree, E.F.,197248422427Hinnen, A., J.B. Hick and G.R. Fink,19787519291933Jones, R.P., N. Pamment and P.F. Greenfield,1981154249Lee, D.H., K. Uchiyama, S. Shioya and Y.I. Hwang,1998Saccharomyces cerevisiae in batch culture.]]>20160165Marzola, D.L. and D.P. Bartholomew,1979205555559Rossillo-Calle, F., D.O. Hall, A.L. Arora and J.O.B. Carioca,19921992pp: 23-54pp: 23-54Smith, B.W. and J.H. Roe,19481795359Steyn, A.J.C. and I.S. Pretorious,1990Saccharomyces cerevisiae.]]>576126Thomsen, K.K.,1983Saccharomyses cerevisiae is released in the culture medium.]]>48545555Uchiyama, K., T. Ohtani, M. Morimoto, S. Shioya, K. Suga, S. Harashima and Y. Oshima,1995Saccharomyces cerevisiae for the PHO regulatory system.]]>11510517