Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2000.911.914Salmonella typhi That Causes Typhoid-Enteric Fever]]>KhanMd. Abdul Hye ChowdhouryAsif Hasan MosaddikM. Ashik ShajahanM. 6200036Ten multidrug resistant Salmonella typhi were isolated from the blood and stool samples of clinically suspected patients suffering from typhoid fever. To see whether the drug resistance phenomenon was plasmid mediated, one of these resistant isolates was used for the plasmid analysis and a single 20 kb plasmid was found. This 20 kb plasmid was transferred to a sensitive E. coli LE 392 and after the plasmid transfer experiment E. coli LE 392 acquired resistance against those previously used antibiotics. Plasmid analysis of the transformed E. coli LE 392 further exhibited that it harbored a single 20 kb plasmid corresponding to that of the wild S. typhi which further confirmed that the 20 kb plasmid was carrying the gene(s) responsible for the multidrug resistance in Salmonella typhi.]]>Agarwal, K.C., B.R. Panhotra, J. Mahanta, V.K. Arya and R.K. Garg,1981Salmonella typhi associated with R-plasmid.]]>73484488Alam, M.N., S.A. Hag, M.N. Majid, Z. Hasan, S.A. Ahsan, N. Ahmed and K.M. Rahman,199233641Anderson, E.S. and H.R. Smith,1972Typhoid bacillus.]]>3329331Bauer, A.W., W.M.M. Kirby, J.C. Sherris and M. Turck,196645493496Choudhury, M.R.,19883rd Edn.,pp: 58pp: 58Cohen, S.N., A.C.Y. Chang and L. Hsu,1972Escherichia coli by R-factor DNA.]]>6921102114Colquhoun, J. and R.S. Weetch,1950256621623Edelman, R. and M.M. Levine,19868329349Halder, K.K., B.S. Dalal, E. Ghose and S. Sanyal,1992Salmonella typhi: The cause of recent out-break of enteric fever in Calcutta.]]>351117Holmes, D.S. and M. Quigley,1981114193197Mandal, B.K.,199033613831383Sambrook, J., E.F. Fritsch and T.M. Maniatis,19892nd Edn.,Meyers, J.A., D. Sanchez, L.P. Elewell and S. Falkow,197612715291537Mishra, O.P., B.L. Gupta, G. Nath and J. Prakash,199642310311Chcesbrough, M.,19915th Edn.,pp: 58-69, 138-421pp: 58-69, 138-421Morshed, M.G., W.A. Khan, N.Z. Khan and N.S. Akbar,1986Salmonella typhi in Bangladesh.]]>4241241Olarte, J. and E. Galindo,1973Salmonella typhi resistant to chloramphenicol, ampicillin and other antimicrobial agents: Strains isolated during an extensive typhoid fever epidemic in Mexico.]]>4597601Pandey, K.K., S. Srinivasan, S. Mahadevan, P. Nalini and R.S. Rao,199027153156Singh, M.,199128329332