Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.1998.321.325AltafN. IqbalJ. AhmadM. S. 4199814Culture responses of 9 lentil explants were compared in various media having different ingredients. The most promising were the scrapped axillary portions of basal nodes for enhanced regeneration. The optimum size of the explant was also determined. The callogenesis response varied with the explant and ranged between 0.3-0.5 cm.
Among the sugars tested, sucrose was the best for callussing. Auxin 2,4-D induced callussing but cytokinin enhanced formationof shoot primordia in explants. Both BA and K promoted multiple shoot formation but K and GA gave more shoot buds. Cytokinins with auxins gave callusing. Callus was also induced in explants when grown in K (10 mg L1) + GA (1 mg L1) containing medium in the dark. When callus was shifted to light it started regeneration as green dots. The addition of seed extract to K and GA medium considerably enhanced regenerations.]]>GOP.,19961996Crocomo, O.J., W.R. Sharp and J.E. Peters,1976Phaseolus vulgaris with the addition of a bean seed extract.]]>78456460Dryden, R.D. and C.H. Karishnamurthy,197791418FAO.,1988FAO.,1992Franclet, A., M. Boulay, F. Bekkaoui, Y. Fouret, B. Verschoore-Martouzet and N. Walker,19871987pp: 232-248pp: 232-248Gamborg, O.L., R.A. Miller and K. Ojima,196850151158Gulati, A. and P.K. Jaiwal,1994Vigna radiata (L.) Wilczek).]]>13523527Jackson, J.A. and S.L.A. Hobbs,1990Pisum sativum L.).]]>26825835Murashige, T. and F. Skoog,196215473497Nadolska-Orczyk, A. and W. Orczyk,199480137143Plata, E. and A.M. Vieitez,1990In vitro regeneration of Camellia reticulata by somatic embryogenesis.]]>65707714Polanco, M.C., M.I. Pelaez and M.L. Ruiz,1988in vitro cultures of Lens culinaris Medik.]]>15175182Williams, D.J. and A. McHughen,1986Lens culinaris Medik. (lentil) in vitro.]]>7149153