Journal of Fisheries and Aquatic Science1816-49271996-0751Academic Journals Inc.10.3923/jfas.2009.306.315Salmo trutta caspius)]]>BahrekazemiM. MatinfarA. SoltaniM. AbtahiB. PustiI. MohagheghiA. 6200946The effects of egg retention time in the abdominal cavity after ovulation on egg viability were studied in Caspian brown trout (Salmo trutta caspius). Eggs were retained in the parental abdominal cavity for 40 days post ovulation. Partial volumes of eggs stripped from 10 individually identified females and fertilized with fresh semen obtained from 8 males at 10 days intervals for 4 stages. The biochemistry of the eggs and ovarian fluid were studied to investigate possible links with post-ovulatory oocyte aging. The eyeing and hatching rate of the eggs declined with over-ripening time: that is, the expected amounts (90.60±6.28% for eyeing and 86.33±6.82% for hatching) in newly ovulated eggs (0-10 days post ovulation) decreased to 0.67±1.34% and 0.49±0.98%, respectively, in over-ripened eggs (30-40 days post ovulation). However, larval abnormalities remained constant for 30 days after ovulation. Over the course of post-ovulation oocyte aging, the pH of the ovarian fluid significantly decreased and the concentration of glucose, protein, calcium, iron and aspartate aminotransferase activity significantly increased. Moreover, the concentration of protein, triglycerides and aspartate aminotransferase activity in the eggs also changed. The present study demonstrated that the best time to take Caspian brown trout eggs after ovulation at 7±0.6°C was up to 10 days post ovulation. Also, egg viability was related to both ovarian fluid parameters (e.g., pH, protein, aspartate aminotransferase, glucose, cholesterol, triglycerides, iron, calcium) and egg parameters (e.g., cholesterol, triglycerides, iron, aspartate aminotransferase) which can be used to detect egg quality defects associated with oocyte post-ovulatory aging.]]>Aegerter, S. and B. Jalabert,2004Oncorhynchus mykiss).]]>2315971Azuma, T., H. Ohta, S. Oda, K. Muto, T. Yada and T. Unuma,200369131136Bauer, P.J.,19811106172Bergmeyer, H.U., M. Horder and R. Rej,198624497510Bonnet, E., B. Jalabert and J. Bobe,2003in vitro storage of rainbow trout (Oncorhynchus mykiss) unfertilised eggs in coelomic fluid at 12°C does not affect development success.]]>274751Burtis, C.A. and E.R. Ashwood,1996Craik, J.C.A. and S.M. Harvey,1984Salmo gairdneri).]]>37347357Evans, D.H. and J.B. Claiborne,20063rd Edn.,Pages: 587Pages: 587Fauvel, C., M.H. Omnes, M. Suquet and Y. Normant,1993Scophthalmus maximus) by a simple pH measurement.]]>117107113Gaudemar, B.D.E. and E. Beall,199853434446Hirao, S., J. Yamada and R. Kikuchi,195421240243Lahnsteiner, F.,200023107118Lahnsteiner, F., T. Weismann and R.A. Patzer,1995Salmonid species: Oncorhynchus mykiss, Salmo trutta lacustris, Salvelinus alpinus and Hucho hucho.]]>35465474Lahnsteiner, F., T. Weismann and R.A. Patzner,1997Alburnus alburnus) Teleostei, Cyprinidae.]]>29305314Lahnsteiner, F., T. Weismann and R.A. Patzner,1999Salmo trutta Lacustris).]]>20375388Lahnsteiner, F., B. Urbanyi, A. Harvath and T. Weismann,2001195331352Matsubara, T., A. Hara and K. Takano,1985Oncorhynchus keta).]]>81309314McPherson, R.A. and M.R. Pincus,200724th Edn.,pp: 1395pp: 1395Mohagheghi, A., M.R. Ahmadi, T. Azuma, G.R. Rafiee, B. Mojazi Amiri and M.R. Naghavi,2008Oncorhynchus mykiss) under cold temperatures.]]>278195198Mojazi, A.B., M. Bahrekazemi, I. Pousti and A.S. Vilaki,2005Salmo trutta caspius (Kessleri), from hatching to parr stage.]]>56384Mommsen, T.P. and T.W. Moon,2005pp: 435pp: 435Rifai, N., G.R. Warnick and M.H. Dominiczak,19972nd Edn.,pp: 189-206pp: 189-206Rime, H., N. Guitton, C. Pineau, E. Bonnet, J. Bobe and B. Jalabert,200422626Springate, J.R.C., N.R. Bromage, J.A.K. Elliott and D.L. Hudson1984Salmo gairdneri).]]>43313322Tietz, N.W.,1986pp: 583-584pp: 583-584