Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2017.21.27Trichoderma harzianum]]>PertiwiningrumAmbar BudyantoEdy Cahyo HidayatMuslikhin Rochijan SoehermanYudistira HabibiMochammad Fahmi 12017171Background and Objective: The sludge from biogas production can be used as organic fertilizer and carrier agent of Trichoderma harzianum which functions as plant growth stimulator and bio-fungicide. This study aimed to determine the effect of different volumes of Trichoderma harzianum inoculum on the concentration of nutrients and organic fertilizer as carrier agent of Trichoderma harzianum. Methodology: Rice husk charcoal and Trichoderma harzianum inoculum in broken corn were added to the sludge. This study applied four treatments with the addition of different volumes of inoculum and do repetitions of each treatment three times. Testing the ability of the mixture in storing Trichoderma harzianum tests 8 times once a week. Testing the ability of Trichoderma harzianum in decomposing organic materials until they are ready to use the test be repeated three times. The variables observed were the total volume of nitrogen, phosphorus, potassium and conidiophores. Data obtained were examined by ANOVA analysis and conducted the Duncan test if there are differences between treatments. Results: The result showed that sludge could not yet be used as organic fertilizer since the result was below 4. The addition of inoculum did not guarantee the higher amount of living conidiophores. On the contrary, the less inoculum was given the more conidia could survive especially in the 4th and 5th week. The mixture could keep Trichoderma harzianum optimally until the 6th week since it was incubated. The result of in vivo test to mustard green plant the result showed no significant difference. Conclusion: The conclusion of the study is the increase in concentration nutrient (N, P and K) and the formula which had the highest average value was T3 (with the addition of 112.5 g of Trichoderma harzianum). Every time a number of conidia/conidiophores in each treatment was measured, the results fluctuated. In vivo test showed no significant result from all given formulas.]]>Gordon, S.B., N.G. Bruce, J. Grigg, P.L. Hibberd and O.P. Kurmi et al.,20142823860Lu, X., D. Han and Z. Huang,201137741783Akhtar, J. and N.A.S. Amin,20111516151624Raheem, A., W.W. Azlina, Y.T. Yap, M.K. Danquah and R. Harun,201549990999Yentekakis, I.V., T. Papadam and G. Goula,200817915211525Bakhov, Z.K., K.U. Korazbekova and K.M. Lakhanova,20141710231029Bond, T. and M.R. Templeton,201115347354Sutaryo, S., A.J. Ward and H.B. Moller,2014398390Wahyuni, S.,2012Pages: 104Pages: 104Simamora, S., Salundik, S. Wahyuni and Surajudin,2006Antoni, D., V.V. Zverlov and W.H. Schwarz,2007772335Weiland, P.,201085849860Soenandar, M. and R.H. Tjachjono,2012Pages: 126Pages: 126Umrah, T. Anggraeni, R.R. Esyanti and I.N.P. Aryantha,2009Trichoderma sp. in controlling Phytophthora palmivora development on cocoa pod].]]>16916Parawira, W.,20098187200Surendra, K.C., D. Takara, A.G. Hashimoto and S.K. Khanal,201431846859Moussa, T.A.A., 2002Rhizoctonia solani Kuhn.]]>2800804Attitalla, I.H. and B. Salleh,2010Trichoderma harzianum.]]>9529532Howell, C.R.,2003Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts.]]>87410Kucuk, C. and M. Kivanc,2004In vitro antifungal activity of strains of Trichoderma harzianum.]]>28111115Harman, G.E.,2006Trichoderma spp.]]>96190194Mir, G.H., L.S. Devi, S. Ahmad, V.M. Kumar and P. Williams,2011Trichoderma viride on corm rot pathogen complex of saffron (Crocus sativus) in Kashmir.]]>107378Sitepu, H., U. Suryanti and S. Purwantisari,2011295057Busko, M., J. Chełkowski, D. Popiel and J. Perkowski,2007Trichoderma on trichothecene mycotoxin production by Fusarium species.]]>88536541