Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2016.1.11Vibrio alginolyticus Strain VA2 as Vaccine Candidates in Tiger Grouper Culture]]>NehlahR. Ina-SalwanyM.Y. ZulperiZ. 1-22016161-2Vibriosis is one of the most prevalent fish diseases caused by bacteria from the genus Vibrio. An effective method to counter Vibriosis is vaccination which increases the resistance of the immune system to subsequent pathogen infection. In this study, Vibrio alginolyticus strain VA2 isolated from infected tiger groupers was selected for determination of the antigenicity and molecular characterization of its Outer Membrane Proteins (OMPs). Protein profiling of OmpK and OmpW were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) while their antigenicity were tested by Western Blot. Results of the Western Blot assay revealed three antigenic OMP bands about 23, 31 and 34 kDa. Sequencing results showed that the full length of the target genes OmpK and OmpW of V. alginolyticus strain VA2 were 846 and 642 bp, respectively. Multiple alignment analysis showed that both regions of OmpK and OmpW were highly conserved among Vibrio species, thus are good candidates for vaccine development. Phylogenetic analysis of OmpK and OmpW showed that most branches in which they were grouped with had bootstrap values higher than 50%, indicating high confidence level of the branches. The antigenic sites of OmpK and OmpW proteins were predicted which were 34 and 27, respectively, thus are good candidates for vaccine development.]]>Cai, S.H., Y.S., Lu, Z.H. Wu, J.C. Jian, B. Wang and Y.C. Huang,2010Vibrio alginolyticus, the causative agent of vibriosis in mariculture fish.]]>50480485Cai, S.H., Y.S. Lu, J.C. Jian, B. Wang and Y.C. Huang et al.,2013Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene.]]>1063947Defoirdt, T., N. Boon, P. Sorgeloos, W. Verstraete and P. Bossier,200725472479Gatewood, D.M., B.W. Fenwick and M.M. Chengappa,1994Pasteurella haemolytica A1 outer membrane proteins, capsule and leukotoxin.]]>41221233Hamod, M.A., M.S. Nithin, Y.N. Shukur, I. Karunasagar and I. Karunasagar,2012V. anguillarum.]]>354-355107110Harikrishnan, R., C. Balasundaram and M.S. Heo,2010309114Khushiramani, R., S.K. Girisha, I. Karunasagar and I. Karunasagar,2007Aeromonas hydrophila and study of immunogenicity in fish.]]>51303307Kurupati, P., B.K. Teh, G. Kumarasinghe and C.L. Poh,2006Klebsiella pneumoniae clinical strain by immunoproteome analysis.]]>6836844Li, N., Z. Yang, J. Bai, X. Fu, L. Liu, C. Shi and S. Wu,2010Vibrio species: Outer membrane protein-OmpK as a versatile Vibriosis vaccine candidate in orange-spotted grouper (Epinephelus coioides).]]>28952956Lin, Y.H., H.Y. Lin and S.Y. Shiau,2012Epinephelus malabaricus according to physiological and biochemical parameters.]]>324-3259296Liu, H., Q. Wang, Q. Liu, X. Cao, C. Shi and Y. Zhang,2011Vibrio alginolyticus and its potential application as a target for live attenuated vaccine.]]>91353364Lun, J., C. Xia, C. Yuan, Y. Zhang, M. Zhong, T. Huang and Z. Hu,2014Vibrio species.]]>32809815Maiti, B., P. Raghunath, I. Karunasagar and I. Karunasagar,2009Aeromonas hydrophila and study of its distribution in Aeromonas spp.]]>10711571167Maiti, B., M. Shetty, M. Shekar, I. Karunasagar and I. Karunasagar,2012149298301Mao, Z., L. Yu, Z. You, Y. Wei and Y. Liu,2007Vibrio parahaemolyticus zj2003.]]>23567575Nandi, B., R.K. Nandy, S. Mukhopadhyay, G.B. Nair, T. Shimada and A.C. Ghose,2000Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW.]]>3841454151Ningqiu, L., B. Junjie, W. Shuqin, F. Xiaozhe, L. Haihua, Y. Xing and S. Cunbin,2008OmpK, is an effective vaccine candidate for Vibrio harveyi in Orange-spotted grouper (Epinephelus coioides).]]>25829833Qian, R., W. Chu, Z. Mao, C. Zhang, Y. Wei and L. Yu,2007Vibrio alginolyticus.]]>39194200Qian, R.H., Z.H. Xiao, C.W. Zhang, W.Y. Chu and L.S. Wang et al.,2008Vibrio alginolyticus.]]>27859Qian, R., Z. Xiao, C. Zhang, W. Chu, Z. Mao and L. Yu,2008Vibrio alginolyticus.]]>24245251Sabri, M.M.Y.,1999Pasteurella haemolytica A2, A7 and A9 in sheep.]]>1999Sambrook, J. and D.W. Russell,20013rd Edn.,Pages: 2344Pages: 2344Shapawi, R., I. Ebi, A.S.K. Yong and W.K. Ng,2014Epinephelus fuscoguttatus (Forskal), by varying the proportion of dietary protein and lipid levels.]]>19198105Zhang, C., L. Yu and R. Qian,2007Vibrio harveyi outer membrane proteins: Their immunoprotective ability against vibriosis in large yellow croaker (Pseudosciaena crocea).]]>10315871599