Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2010.131.137In vitro Regeneration of Citrullus lanatus cv. Round Dragon]]>GanasanKavitah HuyopFahrul 22010102The objective of the current study was to establish an efficient and reproducible in vitro plant regeneration protocol using cotyledonary explant for Citrullus lanatus cv. Round Dragon. To achieve optimal conditions for adventitious shoot induction, cotyledon explants of 5-day-old seedlings, 7-day-old seedlings and 9-day-old seedlings were tested for regeneration potential on Murashige and Skoog (MS) media supplemented with 2.3 mg L-1 BAP. Results showed that high frequency of in vitro adventitious shoot regeneration was induced from the proximal region of 5-day-old seedlings (93%) with 19.80±0.99 shoots per responding explant after 6 weeks. Adventitious shoots induced from 5-day-old seedlings after 6 weeks were transferred to MS shoot regeneration medium without plant growth regulator for shoot elongation for 4 weeks. The influence of various concentrations of IBA, IAA and NAA on root initiation was examined on half-strength and full-strength of MS rooting medium. The best response for root initiation was obtained from the microshoots grown in full-strength MS rooting medium compared to the half-strength MS rooting medium. Furthermore, IBA was more efficient in promoting root induction than IAA and NAA, resulting in a higher rate of root initiation (100%) at the concentration of 0.1 mg L-1 IBA. Therefore, elongated shoots were rooted in MS medium supplemented with 0.1 mg L-1 IBA for 3 weeks. Rooted plantlets were acclimatized successfully under ex vitro conditions.]]>Adelberg, J.W., B.B. Rhodes and H.T. Skorupska,1993336370380Ahad, A.R., M.H. Islam, K. kuzzaman and O.I. Joarder,199423944Ahn, Y.J., L. Vang, T.A. McKeon and G.Q. Chen,2007Ricinus communis L.).]]>43915Alper, Y., J.W. Adelberg, R.E. Young and B.B. Rhodes,1994in vitro watermelon.]]>3713311336Alper, Y., R.E. Young, J.W. Adelberg and B.B. Rhodes,19943713371343Ananthakrisnan, G., X. Xia, C. Elman, S. Singer, H.S. Paris, O.A. Gal and V. Gaba,2003Cucurbita pepo) by in vitro organogenesis.]]>21739746Barnes, L.R.,1979In vitro propagation of watermelon.]]>11223227Blackmon, W.J. and B.D. Reynolds,1982In vitro shoot regeneration of Hibiscus acetosella, muskmelon, watermelon and winged beans.]]>17558589Chaturvedi, R. and S.P. Bahtnagar,200137255258Choi, P.S., W.Y. Soh, Y.S. Kim, O.J. Yoo and J.R. Liu,1994Agrobacterium tumafaciens.]]>13344348Compton, M.E. and D.J. Gray,1993126165Compton, M.E., D.J. Gray and G.W. Elmstrom,199333211217Compton, M.E., D.J. Gray and G.W. Elmstrom,1996in vitro.]]>87165172Compton, M.E.,199732514515Compton, M.E.,199958185188Compton, M.E., 200035749750Dabauza, M., M. Bordas, A. Salvador, L.A. Roig and V. Moreno,1997Agrobacterium-mediated transformation of cotyledon explants of Citrullus colocynthis (L.) Schrad.]]>16888892Debeaujon, I. and M. Branchard,19933491100Dong, J.Z. and S.R. Jia,1991Citrullus vulgaris).]]>9559562Gaba, V., E. Schlarman, C. Elman, O. Sagee, A.A. Watad and D.J. Gray,1999In vitro studies on the anatomy and the morphology of bud regeneration in melon cotyledons.]]>3517Jaworski, J.M. and M.E. Compton,199732469470Krug, M.G.Z., L.C.L. Stipp, A.P.M. Rodriguez and B.M.J. Mendes,2005In vitro organogenesis in watermelon cotyledons.]]>40861865Pech, J.C., A. Bernadac, M. Bouzayen, A. Latche, C. Dogimont and M. Pitrat,200760209240Pirinc, V., A. Onay, H. Yildrim, F. Adiyaman, C. Isikala and D. Basaran,2003Citrullus lanatus cv. Surme).]]>27101105Shin, D.H., J.S. Kim, I.J. Kim, J. Yang, S.K. Oh, G.C. Chung and K.H. Han,2000Helianthus annuus L.).]]>36273278Srivastava, D.R., V.M. Andrianov and E.S. Piruzian,1989Citrullus vulgaris Schrad. cv. Melitopolski).]]>8300302Sultana, R.S., M.A. Bari, M.H. Rahman, M.M. Rahman, N.A. Siddique and N. Khatun,2004In vitro rapid regeneration of plantlets from leaf explant of watermelon (Citrullus lanatus Thumb.).]]>3131135Thakur, A.K., S. Sharma and D.K. Srivastava,2005Himalayan poplar (Populus ciliata Wall.).]]>89664668