International Journal of Poultry Science1682-83561994-7992Asian Network for Scientific Information10.3923/ijps.2017.344.353AttiaG. El-ErakyW. HassaneinE. El-GamalM. FarahatM. Hernandez-SantanA. 92017169Objective: This trial was conducted to determine if the growth performance, apparent nutrient digestibility, caecal microflora and intestinal histomorphology of broiler chickens could be enhanced via supplementation of their diets with a blend of plant extracts containing oregano, fenugreek, chamomile and fennel. Methodology: The plant extract blend was included as a natural growth promoter in six dietary treatments at levels of 0, 100, 200, 500, 1000 and 2000 ppm. A seventh treatment, supplemented with 200 ppm oxytetracycline (OTC) antibiotic as growth promoter, was included in the study design to compare its effect to those fed on the plant extract blend (0 and 200 ppm). The trial utilized 245, day-old chicks distributed in 7 dietary treatments (7 birds/pen; 5 replicate pens/treatment). Results: No significant differences were observed in the measured growth performance parameters (body weight, daily gain, daily feed intake and feed conversion ratio) due to inclusion of the plant extract blend (p>0.05). Supplementation of the broiler diet with 200 ppm of OTC resulted in significant improvement in the measured growth performance parameters compared to those fed on 0 (control) or 200 ppm of the plant extract blend. The mortality percentage in the control group was 11.4% and was decreased by an average of 50-75% in broilers fed on the plant extract blend. No mortality was observed in broilers fed on the 200 ppm OTC. The apparent total tract digestibility of dry matter, crude protein and ether extract was increased due to inclusion of the plant extract blend or OTC. The caecal microflora count was positively influenced (p<0.05) by inclusion of the plant extract blend (decreased coliforms and increased lactobacilli count). Dietary intake of OTC decreased both coliforms and lactobacilli. The intestinal histomorphological parameters (villus height, crypt depth and villus height-to-crypt depth ratio) were not significantly influenced by inclusion of the plant extract blend or OTC in the feed. Conclusion: The utilized plant extract blend showed the capacity to significantly improve total tract apparent nutrient digestibility and caecal microflora count, but without an observed improvement in the growth performance and intestinal histomorphology.]]>Cromwell, G.L.,200213727Yang, Y., P.A. Iji and M. Choct,20096597114Adam, K., A. Sivropoulou, S. Kokkini, T. Lanaras and M. Arsenakis,1998Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia and Salvia fruticosa essential oils against human pathogenic fungi.]]>4617391745Yoshino, K., N. Higashi and K. Koga,200652169173Preuss, H.G., B. Echard, M. Enig, I. Brook and T.B. Elliott,20052722934Giannenas, I., P. Florou-Paneri, M. Papazahariadou, E. Christaki, N.A. Botsoglou, and A.B. Spais,2003Eimeria tenella.]]>5799106Platel, K. and K. Srinivasan,2004119167179Roofchaee, A., M. Irani, M.A. Ebrahimzadeh and M.R. Akbari,2011Origanum vulgare L.) essential oil on growth performance, cecal microflora and serum antioxidant activity of broiler chickens.]]>1061776183Alp, M., M. Midilli, N. Kocabagli, H. Yilmaz, N. Turan, A. Gargili and N. Acar,201221630636Reisinger, N.T. Steiner, S. Nitsch, G. Schatzmayr and T.J. Applegate,201120272283Mullaicharam, A.R., G. Deori and R.U. Maheswari,2013413041313Ahmadi, F., M.B.V. Sara, E.S. Naghani and B. Shokrollahi,20122012pp: 15Alkofahi, A., R. Batshoun, W. Owais and N. Najib, 199667435442Safaei, A., S.M. Rahanjam and M. Gharajanlu,2013Trigonella foenum-graecum on immune response and some blood parameters of broilers.]]>3117120Sauvaire, Y., P. Petit, Y. Baissac and G. Ribes,20002000pp: 107-109pp: 107-109Alloui, N., B.S. Aksa, M.N. Alloui and F. Ibrir,2012Trigonella foenum-graecum) as growth promoter for broiler chickens.]]>22527Weerasingha, A.S. and N.S.B.M. Atapattu,2013Fenugreek (Trigonella foenum-graecum L.) seed powder on growth performance, visceral organ weight, serum cholesterol levels and the nitrogen retention of broiler or chicken.]]>24289295Salamon, I.,19921014Carle, R., I. Fleischhauer and D. Fehr,198712724512457Newall, C.A., L.A. Anderson and J.D. Phillipson,19962nd Edn.,Pages: 296Pages: 296Singh, O., Z. Khanam, N. Misra and M.K. Srivastava,2011Matricaria chamomilla L.): An overview.]]>58295Al-Kaisse, G.A.M. and E.K. Khalel,2011Matericaria chamomilla L.) as feed supplements (Growth promoters) on productive performance and hematological parameters constituents of broiler.]]>10726729Abaza, I.M., M.A. Asar, G.E. Elshaarrawi and M.F. Hassan,200381735750ESCOP.,2003Pages: 556Pages: 556Satyanarayana, S., K. Sushruta, G.S. Sarma, N. Srinivas and G.V. Subbva Raju,2004in-vitro systems.]]>4110Kwon, Y.S., W.G. Choi, W.J. Kim, W.K. Kim, M.J. Kim, W.H. Kang and C.M. Kim,2002Foeniculum vulgare.]]>25154157El-Deek, A.A., Y.A. Attia and M.M. Hannfy,2002Pimpinella anisum), ginger (Zingiber officinale roscoe) and fennel (Foeniculum vulgare) and their mixture on performance of broilers.]]>679296Mohammed, A.A. and R.J. Abbas,2009Foeniculum vulgare L.) on productive performance of broiler chickens.]]>8642644NRC.,19949th Edn.,Pages: 176Pages: 176AOAC.,199015th Edn.,Khadem, A., L. Soler, N. Everaert and T.A. Niewold,2014Macleaya cordata extract is based on their anti-inflammatory properties.]]>11211101118Short, F.J., J. Wiseman and K.N. Boorman,199979195209Marquardt, R.R.,19836221062108Maynard, L.A., J.K. Loosli, H.F. Hintz and R.G. Waener,19797th Edn.,Pages: 620Pages: 620Murugesan, G.R., B. Syed, S. Haldar and C. Pender,20152015Aptekmann, K.P., S.M.B. Arton, M.A. Stefanini and M.A. Orsi,2001Coturnix coturnix japonica) treated with different levels of dietary calcium.]]>30277280Analytical Software,2008Basmacioglu, H., O. Tokusoglu and M. Ergul,200434197210Fukayama, E.H., A.G. Bertechini, A. Geraldo, R.K. Kato and L.D.S. Murgas,20053423162326Barreto, M.S.R., J.F.M. Menten, A.M.C. Racanicci, P.W.Z. Pereira and P.V. Rizzo,200810109115Jacubcova, Z., L. Zeman, P. Horky, E. Mrkvicova, P. Mares, E. Mrazkova and O. Stastnik,20142014147150Lihua, C., Y. Ying, L. Yifu and C. Lei,20075911Elbushra, M.E.,2012Trigonella foenum) as natural feed addition on broiler chicks performance.]]>132731Mahmud, H.A.,201434795807Zulkifli, I., N. Abdullah, N.M. Azrin and Y.W. Ho,2000Lactobacillus cultures and oxytetracycline under heat stress conditions.]]>41593597Kalavathy, R., N. Abdullah, S. Jalaludin, C.M.V.L. Wong and Y.W. Ho,2008Lactobacillus cultures and oxytetracycline on the growth performance and serum lipids of chickens.]]>7385389Lee, K.W., H. Everts, H.J. Kappert, M. Frehner, R. Losa and A.C. Beynen,200344450457Windisch, W., K. Schedle, C. Plitzner and A. Kroismayr,200886E140E148Hernandez, F., J. Madrid, V. Garcia, J. Orengo and M.D. Megias,200483169174Bin-Hafeez, B., R. Haque, S. Parvez, S. Pandey, I. Sayeed and S. Raisuddin,2003Trigonella foenum graecum L.) extract in mice.]]>3257265Ferket, P.R., C.W. Parks and J.L. Grimes,20022002Apajalahti, J., A. Kettunen and H. Graham,200460223232Rolfe, R.D.,2000130396S402SPenalver, P., B. Huerta, C. Borge, R. Astorga, R. Romero and A. Perea,2005Enterobacteriaceae family.]]>11316Helander, I.M., H.L. Alakomi, K. Latva-Kala, T. Mattila-Sandholm and I. Pol et al.,19984635903595Cross, D.E., R.M. McDevitt, K. Hillman and T. Acamovic,200748496506Amat, C., J.M. Planas and M. Moreto,1996271R1085R1089Savage, T.F., E.I. Zakrzewska and J.R. Andreasen,199776139139Geyra, A., Z. Uni and D. Sklan,200180776782Khattak, F., A. Ronchi, P. Castelli and N. Sparks,201493132137Mairoka, A., A.M.E. Santin, S.A. Borges, M. Opalinski and A.V.F. Silva,200493137Gunal, M., G. Yayli, O. Kaya, N. Karahan and O. Sulak,20065149155