International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2017.516.528Abdel-GhaffarOsama MahmoudSalwa Thabet Ali SaidAzza Abdel-Azeem Youssef SanadFatma 62017136Background and Objective: Isoniazid (INH) still represents a first-line drug for the treatment and prophylaxis of tuberculosis but different adverse reactions frequently develop in patients administered this drug. Rutin is a common dietary flavonoid consumed in fruits, vegetables and plant-derived beverages. Rutin showed in different reports anti-inflammatory and antioxidative properties. The aim of this study was to evaluate the antioxidative activity of rutin against the INH-induced hepatic toxicity using certain physiological criteria corroborated with a histopathological study of the liver. Materials and Methods: Eighty male albino rats were randomly divided into 4 groups: Control, INH-treated, (rutin+INH)-treated and rutin-treated groups. The INH and rutin were orally administered at dose levels of 54 and 40 mg kg1 b.wt., respectively, daily for 4 weeks. Liver function markers were determined in serum in addition to the hepatic malondialdehyde (MDA) and glutathione (GSH) concentrations and superoxide dismutase (SOD) activity. These were supported with a histopathological study of the liver. Statistical analysis was carried out using t-test and analysis of variance (ANOVA). Results: Serum albumin concentration was reduced in INH-treated rats while the level of serum globulin was increased. So, serum albumin/globulin(A/G) ratio was significantly reduced (p<0.05). The activities of serum aspartate and alanine aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) were significantly elevated(p<0.05) in association with INH administration being indicative to the liver affection. 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