International Journal of Dairy Science1811-97431811-9751Asian Network for Scientific Information10.3923/ijds.2014.56.62NawangsariDwi Novrina Al-BaarriAhmad Ni`matullah MulyaniSri LegowoAnang Mohamad BintoroV. Priyo 2201492Lactoperoxidase (LPO) could be simply obtained from whey through immobilization
using a cation exchange resin of SP Sepharose. LPO received high attention since
the antimicrobial properties of LPO system (LPOS) that are consisted of LPO,
SCN¯ and H2O2 was able to generate OSCN¯ for
strong antimicrobial agents. This study was done to analyze the immobilization
efficiency of LPO onto two types of sepharose: SP-Sepharose Fast Flow (SPFF)
and SP-Sepharose Big Beads (SPBB). The remaining of LPOs activity (%)
against storage solution was also observed. The whey was obtained from bovine
skimmed milk that was coagulated using rennet and acid lactic. The LPO was obtained
from whey using SPFF. To analyze the resistence of enzyme activity, the immobilized
LPO was stored in pure water, phosphate buffer, milk and whey at 10°C. The
activity of LPO was monitored for 10 days. The result indicates that the LPO
could be purified from whey and single band has been detected using electrophoresis
method. The obtained LPO (35 U mL-1) was attached onto SPFF and SPBB.
Maximum immobilization efficiency has been achieved by 0.6 g SP-FF and 0.9 g
SP-BB. LPO activity of the immobilized LPO were able to be kept until 5 days
when it was stored in whey. Other storage solution remained various LPO activity
during storage. This study concluded that IE of LPO attached onto sepharoses
might be reached variously depended on the sepharose type and whey as LPO source
might be used for maintaining LPO activity.]]>Al-Baarri, A.N., M. Hayashi, M. Ogawa and S. Hayakawa,201174134139Al-Baarri, A.N., M. Ogawa and S. Hayakawa,201035185191Al-Baarri, A.N., M. Ogawa and S. Hayakawa,201167278Al-Baarri, A.N., M. Ogawa, T. Visalsok and S. Hayakawa,2012146Amersham-Bioscience,20012001Oghaiki, N.A., F. Fonteh, P. Kamga, S. Mendi and H. Imele,20077114Boots, J.W. and R. Floris,20061612721276FAO/WHO,20052005Fee, C.J. and A. Chand,200648143149Fweja, L.W.T., M.J. Lewis and A.S. Grandison,20089125662574Hayashi, M., S. Naknukool, S. Hayakawa, M. Ogawa and A.B.A. Ni'matulah,2012130541546Seifu, E., E.M. Buys and E.F. Donkin,200414581589Seifu, E., E.M. Buys and E.F. Donkin,200516137154Singh, A.K., N. Singh, S. Sharma, K. Shin and M. Takase et al.,200996646654Touch, V., S. Hayakawa, S. Yamada and S. Kaneko,2004Salmonella enteritidis in animal or vegetable foods.]]>93175183Zhou, Y. and L.T. Lim,200974C170C176