International Journal of Dairy Science1811-97431811-9751Asian Network for Scientific Information10.3923/ijds.2007.275.280Lactobacillus plantarum KCA-1: A Potential Probiotical Agent]]>AnukamKingsley C.KoyamaTara E3200723A previously characterized biosurfactant-producing Lactobacillus plantarum KCA-1 isolated from the vagina of a healthy premenopausal woman was used in this study. Lactobacillus plantarum KCA-1 was tested for survival in different concentrations (0, 0.05, 0. 1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0) of porcine and oxgall bile extract, incubated at 37°C for 48 h. Acid tolerance of the culture was studied by inoculating the organism (105CFU mL-1) in de Man Rogas Sharpe (MRS) broth at different pH values adjusted with HCl and incubated at 37°C for 48 h. Lactobacillus plantarum strain KCA-1 that produced biosurfactant needed for preventing adhesion of pathogens was tolerant to oxgall bile. There was an increase of 5 log cycles from the initial bacterial inocula (1x105cfu mL-1) to 1x1010cfu mL-1 at 0.05 and 0.1% concentrations of oxgall bile and 4 log cycle increase (1x109cfu mL-1) at 0.5% oxgall concentration. There was a consistent reduction of 1 log cycle of every 0.5% increase of the oxgall for tested concentrations (0.5 to 4.0%). In addition, Lactobacillus plantarum KCA-1 was tolerant at pH 2.5 for 48 h of incubation as there was 2.3 to 3.8 increase in the log cycles. There was a corresponding increase in the number of cells from 1x105 CFU to 1x109 CFU mL-1 at pH 4.5. The tested potential probiotic strain Lactobacillus plantarum KCA-1 exhibited a satisfactory degree of acid and bile tolerance. The findings suggests that the strain may be a promising candidate for use as a dietary adjunct, but more studies are needed.]]>Anukam, K.C., E.O. Osazuwa, I. Ahonkhai and G. Reid,2005Lactobacillus species from the vagina of healthy Nigerian women.]]>412221227Anukam, K.C., E.O. Osazuwa, I. Ahonkhai, M. Ngwu, G. Osemene, A.W. Bruce and G. Reid,2006Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14: Randomized, double-blind, placebo controlled trial.]]>814501454Anukam, K.C. and G. Reid,2007Lactobacillus plantarum and Lactobacillus fermentum with probiotic potentials isolated from the vagina of healthy Nigerian women.]]>28187Corzo, G. and S.E. Gilliland,1999Lactobacillus acidophilus based on disappearance of conjugated bile salts.]]>82466471Fernandez, M.F., S. Boris and C. Barbes, 200394449455Flahaut, S., J. Frere, P. Boutibonnes and Y. Auffray,1996Enterococcus faecalis.]]>6224162420Golden, B.R. and S.L. Gorbach,19921992pp: 355-376pp: 355-376Hofmann, A.,19913567580Hutt, P., J. Shchepetova, K. Loivukene, T. Kullisaar and M. Mikelsaar,200610013241332Kociubinsky, G., P. Perez and G. De Antoni,199962905912Kurdi, P., K. Kawanishi, K. Mizutani and A. Yokota,200618819791986Limdi, J.K., C. O'Neill and J. Mclaughlin,20061254475457Liong, M.T. and N.P. Shah,2005885566Reid, G., J. Jass, M.T. Sebulsky and J.K. McCormick,200316658672Reid, G. and A.W. Bruce,2006242832Taranto, M.P., G. Perez-Martinez and G. Font de Valdez,2006157720725Usman, A.H.,1999Lactobacillus gasseri strains.]]>82243248FAO/WHO.,20012001