International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2014.74.80In vitro Anticancer Activity and Cytotoxicity of Solanum nigrum on Cancers and Normal Cell Lines]]>H.O. MogladEhssan M. AbdallaOmer KokoW.S. SaadabiA.M. 22014102In this study, eighty percent methanol and chloroform extracts of leaves and stems of Solanum nigrum were screened for in vitro anticancer activity using PC-3 human prostate cancer cell lines and Hela cervical cancer cells and cytotoxicity assays were performed using MTT assay and 3T3 NIH mouse embryo fibroblast cell line and CC-1, a rat Wistar hepatocyte cell line. All extracts in concentration 100 μg mL-1 showed anticancer activity in PC-3 and Hela and the highest percentage of growth inhibition obtained from stems methanol extracts on Hela 91.11% followed by leaves and stems methanol extracts (74.28 and 80.49, respectively) on PC-3. For cytotoxicity the result obtained indicate that all extracts had non-toxic effect on CC-I and 3T3 cell lines with IC50> 100 μg mL-1, except the leaves methanol extract showed the highest percentage of growth inhibition in 3T3 85.63 with IC50 17.37 μg mL-1. The results obtained indicate that Solanum nigrum leaves and stems methanol extracts have anticancer activity on prostate cancer, cervical cancer and have non-toxic effect on 3T3 and CC-1. This result supports the traditional use of Solanum nigrum for the treatment of cancer in different regions of the Sudan.]]>Al-Qirim, T., S.M. Zaidi, M. Shahwan, G. Shattat and N. Banu,2008Solanum nigrum on immobilization stress induced antioxidant defense changes in rat.]]>314261429Caragay, A.B.,1992466568Conforti, F., G. Ioele, G.A. Statti, M. Marrelli, G. Ragno and F. Menichini,20084633253332Cragg, G.M., D.J. Newman and K.M. Snader,1997605260Dhellot, J.R., E. Matouba, M.G. Maloumbi, J.M. Nzikou and M.G. Dzondo et al.,2006Solanum nigrum L. seed oil.]]>5987991Dzhambazov, B., S. Daskalova, A. Monteva and N. Popov,2002In Vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts.]]>25499504Ferlay, J., F. Bray, P. Pisani and D.M. Parkin,2002Hagerman, A.E.,198713437449Harborne, J.B.,19983rd Edn.,Pages: 302Pages: 302Ibrahim, M.B.,1997Anogeissus leiocarpus on Staphylococcus aureaus, Streptococcus pyogenes, Escherichia coli and Proteus vulgaris.]]>22030Jainu, M. and C.S.S. Devi,2006Solanum nigrum (L.) on experimental ulcer models: Possible mechanism for the inhibition of acid formation.]]>104156163Lau, C.B.S., C.Y. Ho, C.F. Kim, K.N. Leung and K.P. Fung et al.,2004Coriolus versicolor (Yunzhi) extract on human leukemia and lymphoma cells by induction of apoptosis.]]>75797808Mann, A., A. Banso and L.C. Clifford,2008Anogeissus leiocarpus and Terminalia avicennioides.]]>103438Prajapati, N.D. S.S. Purohit, A.K. Sharma and T. Kumar,20031st Edn.,Raju, K., G. Anbuganapathi, V. Gokulakrishnan, B. Rajkapoor, B. Jayakar and S. Manian,2003Solanum nigrum Linn against CCl4-induced hepatic damage in rats.]]>2616181619RICK,20042004Patel, S., N. Gheewala, A. Suthar and A. Shah,2009In-vitro cytotoxicity activity of Solanum nigrum extract against Hela cell line and Vero cell line.]]>13846Handa, S.S., S.P.S. Khanuja, G. Longo and D.D. Rakesh,2008Pages: 260Pages: 260Yanez, J., V. Vicente, M. Alcaraz, J. Castillo, O. Benavente-Garcia, M. Canteras and J.A.L. Teruel,200449191199