Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2017.19.26ElmeerKhaled AlghanemMai Al-LatifiLatifa AlhemairiHayat 12017161Background and Objective: Several DNA markers have been used to detect the genetic variation among date palm cultivars, selecting of molecular markers for their specific purposes is the main challenge for researchers, the aim of this study was to evaluate the efficiency of RAPD and ISSR markers in assessing genetic diversity of date palm. Methodology: The DNA of 18 female date palms, comprising Khalas cultivar representing the diversity of date palm genotypes in Qatar was assessed using 29 ISSR primers and 8 RAPD primers, the data were then computed with the PowerMarker software to detect the major allele frequency, number of alleles, gene diversity and Polymorphism Information Content (PIC) value. Results: Twenty nine ISSR primers produced a total of 132 DNA fragments of which 72 fragments (54.5%) were reproducible polymorphic amplified fragments, while 8 RAPD primers produced a total of 32 DNA fragments, of which 22 fragments (68.75%) were polymorphic across all the genotypes. The most effective ISSR and RAPD primers were BT14 and OPC-11 respectively, which generated 7 bands all of which were 100% polymorphic. The RAPD marker exposed 22% gene diversity and 18% (PIC), while ISSR exposed 14 and 12% gene diversity and Polymorphism Information Content (PIC) respectively. Conclusion: The molecular markers obtained by the ISSR technique revealed a remarkable molecular discrimination among date palm compared to the RAPD, while RAPD provides perfect outcomes for date palms genetic diversity.]]>Munier, P.,198136437450Abdulla, M. and O. Gamal,2010Phoenix dactylifra L.) cultivars by protein, RAPD and ISSR markers in Saudi Arabia.]]>42328Al-Farsi, M.A. and C.Y. Lee,200848877887Chao, C.C.T. and R.R. Krueger,2007Phoenix dactylifera L.): Overview of biology, uses and cultivation.]]>4210771082Sedra, M.H., P. Lashermes, P. Trouslot and M.C. Combes,1998Phoenix dactylifera L.) varieties from Morocco using RAPD markers.]]>1037582Henry, R.J.,1998461107112Mirbahar, A.A., G.S. Markhand, S. Khan and A.A. Abul-Soad,2014Phoenix dactylifera L.) cultivars by RAPD markers.]]>46619625Trifi, M., A. Rhouma and M. Marrakchi,2000Phoenix dactylifera L.) germplasm collection using DNA amplification fingerprinting.]]>20665671Al-Khalifah, N.S. and E. Askari,2003Phoenix dactylifera L.) cultivars from Saudi Arabia by DNA fingerprinting.]]>10712661270Soliman, S.S., B.A. Ali and M.M.M. Ahmed,2003Phoenix dactylifera L.) by RAPD-PCR.]]>28687Karim, K., B. Chokri, S. Amel, H. Wafa, H. Richid and D. Nouredine,20106182186Elshibli, S. and H. Korpelainen,2008Phoenix dactylifera L.) germplasm from Sudan.]]>134251260Bodian, A., M. Nachtigall, L. Frese, M.A. Elhoumaizi, A. Hasnaoui, K.N. Ndir and D. Sane,2014Phoenix dactylifera L.) cultivars from Morocco using SSR markers.]]>2014Al-Ruqaishi, I.A., M. Davey, P. Alderson and S. Mayes,2008Phoenix dactylifera L.) in Oman, based on microsatellite markers.]]>617072Yusuf, A.O., A. Culham, W. Aljuhani, C.D. Ataga, A.M. Hamza, J.O. Odewale and L.O. Enaberue,2015Phoenix dactylifera) germplasm based on microsatellite markers.]]>7121132Elmeer, K. and I. Mattat,20151416241635Liu, K. and S.V. Muse,20052121282129Hammer, Ø., D.A. Harper and P.D. Ryan,20012001Benmoussa, M. and A. Achouch,20056427434Mitra, C., P. Kharb, S. Uppal and S. Jain,2011Phoenix dactylifera L.): A comparative assessment using ISSR and RAPD marker assays.]]>86398402Haider, N., I. Nabulsi and N. MirAli,2012Phoenix dactylifera L.) cultivars in Syria using RAPD and ISSR markers.]]>11224Srivashtav, V.S., C.V. Kapadia, M.K. Mahatma, S.K. Jha, S. Jha and T. Ahmad,2012Phoenix dactylifera L.) in the Kutch region of India using RAPD and ISSR markers.]]>25907915Munshi, A. and G. Osman,2010Phoenix dactylifra L.) cultivars by protein, RAPD and ISSR markers in Saudi Arabia.]]>42328Sabir, J.S.M., S. Abo-Aba, S. Bafeel, T.A. Zari and S. Edris et al.,2014Phoenix dactylifera L.) cultivars from Saudi Arabia using AFLP and ISSR markers.]]>337618Khanam, S., A. Sham, J.L. Bennetgen and A.M.A. Mohammed,2012Phoenix dactylifera L.).]]>612361244El-Rabey, H.,2008Hordium murinum L. complex as reveald by RAPD-PCR and seed storage protein electrophoresis (2007).]]>28145156Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski,19962225238Elmeer, K. and A. Almalki,2011Prosopis cineraria and Prosopis juliflora using ISSR and RAPD techniques.]]>2527534Ajibade, S.R., N.F. Weeden and S.M. Chite,2000Vigna.]]>1114755Galvan, M.Z., B. Bornet, P.A. Balatti and M. Branchard,2003Phaseolus vulgaris L.).]]>132297301