Asian Journal of Plant Pathology1819-1541xxxx-xxxxScience International10.3923/ajppaj.2017.191.198Ralstonia syzygii subsp. syzygii]]>DwimartinaFina ArwiyantoTriwidodo JokoTri 42017114Background and Objective: Sumatra disease caused by Ralstonia syzygii subsp. syzygii is reported to generate severe damage to clove plantation in Indonesia. The chemical control of insect vector has partially been successful yet impacted on environmental problems. Endophytic and rhizobacteria have been reported as potential biocontrol agents for many plant diseases due to safety of human and non-target organisms. The objective of this study was to find the potential endophytic and rhizobacteria for biocontrol of Ralstonia syzygii subsp. syzygii. Methodology: The endophytic and rhizobacteria were screened for their indole acetic acid (IAA) production, phosphate solubility, antibiosis activity and followed by molecular characterization. The experiment was arranged in a Completely Randomized Design (CRD) with 3 replications. The data were subjected to statistical analysis using one-way ANOVA and means were separated by DMRT (p<5%). Results: Forty six bacterial endophytes were successfully recovered from roots, stems, leaves and flowers of the healthy clove. Also, 66 isolates were isolated from the rhizospheric soil. Among them, 24 endophytic and 21 rhizobacteria produced indole acetic acid (IAA). Only rhizobacterial isolates but not endophytes indicated phosphatase activities. The in vitro antibiosis assay against Ralstonia syzygii subsp. syzygii showed that 23 endophytic and 14 rhizobacteria produced inhibition zone. Furthermore, 16S rDNA analysis confirmed that such endophytic and rhizobacteria were identified as Bacillus subtilis subsp. subtilis and Bacillus cereus, respectively. Conclusion: Endophytic and rhizobacteria could produce IAA, dissolve phosphate and generate the biggest inhibition zones against R. syzygii subsp. syzygii while molecular assay identified them as B. subtilis subsp. and B. cereus.]]>Prior, P., F. Ailloud, B.L. Dalsing, B. Remenant, B. Sanchez and C. Allen,2016Ralstonia solanacearum into three species.]]>2016Bennett, C.P.A., P. Hunt and A. Asman,198534487494Danaatmadja, Y., S. Subandiyah, T. Joko and C.U. Sari,2009Ralstonia syzygii.]]>15712Ambawade, M.S. and G.R. Pathad,2013Stenotrophomonas maltophilia BE25 isolated from roots of banana (Musa spp).]]>426442650Patel, P.V. and P.B. Desai,201543441Etesami, H., H.M. Hosseini and H.A. Alikhani,2014Oryza sativa L.).]]>14491503Ismiyatuningsih, I., T. Joko and S. Hartono,2016Pectobacterium atrosepticum in major potato-growing areas in central java province, Indonesia.]]>116Hallmann, J., A. Quadt-Hallmann, W.F. Mahaffee and J.W. Kloepper,199743895914Mehta, S. and C.S. Nautiyal,2001435156Nguyen, C., W. Yan, F. Le Tacon and F. Lapyire,1992Laccaria bicolour (Maire) PD Orton.]]>143193199Joko, T., S. Subandiyah and S. Somowiyarjo,2000Xanthomonas campestris pv. citri.]]>63238Windari, U., T. Joko and S. Subandiyah,20151915Lisboa, M.P., D. Bonteto, D. Bizani, J.A. Henriques and A. Brandelli,2006Bacillus amyloliquefaciens isolated from the Brazilian Atlantic forest.]]>9111118Joko, T., H. Hirata and S. Tsuyumu,2007Dickeya dadantii 3937.]]>73266273Joko, T., H. Hirata and S. Tsuyumu,2007Dickeya dadantii 3937 for in planta fitness.]]>73274280Joko, T., A. Subandi, N. Kusumandari, A. Wibowo and A. Priyatmojo,20144712391250Widyaningsih, S., S.N.H. Utami, T. Joko and S. Subandiyah,20179229238Joko, T., N. Kusumandari and S. Hartono,2011Pectobacterium carotovorum, a causal agent of soft-rot disease on orchid.]]>175459Joko, T., D. Kiswanti, S. Subandiyah and Hanudin,2011Phalaenopsis orchids in Yogyakarta and West Java, Indonesia.]]>2011pp: 255265Mahfut, T. Joko and B.S. Daryono,2016Odontoglossum ringspot virus (ORSV) in Java and Bali, Indonesia.]]>10914Reinhold-Hurek, B. and T. Hurek,201114435443Zinniel, D.K., P. Lambrecht, N.B. Harris, Z. Feng and D. Kuczmarski et al.,20026821982208Bais, H.P., T.L. Weir, L.G. Perry, S. Gilroy and J.M. Vivanco,200657233266Joko, T., M.P. Koentjoro, S. Somowiyarjo, M.S. Rohman, A. Liana and N. Ogawa,20124518101818Ngoma, L., K. Mogatlanyane and O.O. Babalola,2014in vitro study.]]>474563Tsavkelova, E.A., T.A. Cherdyntseva, S.G. Botina and A.I. Netrusov,20071626976Sharma, K.,201131112Malleswari, D. and G. Bagyanarayana,201363037Kloepper, J.W., C.M. Ryn and S. Zhang,2004Bacillus spp.]]>9412591266Sivasakthi, S., G. Usharani and P. Saranraj,2014Pseudomonas fluorescens and Bacillus subtilis: A review.]]>912651277Rani, M.U. and G. Reddy,2011Bacillus cereus and Enterobacter cancerogenus screened for their efficient plant growth promoting traits rhizobacteria (PGPR) and antagonistic traits among sixteen bacterial isolates from rhizospheric soils of pigeon pea.]]>520902094