Asian Journal of Plant Pathology1819-1541xxxx-xxxxScience International10.3923/ajppaj.2017.130.138Colletotrichum gloeosorioides Isolates of Mango]]>SornakiliArchana RathinamPrabukarthikeyan Karthikeyan ThiruvengadumRaguchander KuppusamyPrabakar 32017113Background and Objective: Morphological traits of the genus Colletotrichum are extremely variable and hosts might be infected by a single or multiple species of the pathogen, hence development of species-specific primers has provided a powerful tool for the detection of plant pathogens. The objective of the study was to assess efficiency of Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) primers against C. gloeosporioides in terms of genetic variability. Materials and Methods: Random amplified polymorphic DNA and inter simple sequence repeat primers are simple PCR-based assays targeting microsatellite regions of the genome. Intra and interspecific polymorphisms among the twentysix isolates of C. gloeosporioides, causing anthracnose on mango were evaluated by these markers. Results: In the present research, RAPD primers generated 519 amplicans with 82 alleles of which 77 were polymorphic with 7.4 assay efficiency index; ISSR markers produced 1469 amplicans with 189 amplification products, out of which 180 were polymorphic with 11.81 assay efficiency index. Although both the techniques were efficient and reproducible, ISSR indicated higher genetic variability in terms of percent polymorphism, polymorphism information content and effective multiplex ratio compared to RAPD analysis. Among 16 ISSR primers (CAG)5, (TGTC)4, (AGG)5, (TCC)5, (CAG)3, (AG)8 T, (GA)8 T, (TG)8 A, (GA)8 YG, (GT)8 YC recorded highest percent polymorphism and highest PIC value of 0.97 by (GA)8 T and EMR of 23.04 by (GACA)4 was recorded. Conclusion: It is concluded that well-chosen ISSR primers could result in quick estimate in terms of their efficiency in detecting polymorphisms among the isolates.]]>Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski,19962225238Russell, J.R., J.D. Fuller, M. Macaulay, B.G. Hatz, A. Jahoor, W. Powell and R. Waugh,199795714722Davila, J.A., Y. Loarce, L. Ramsay, R. Waugh and E. Ferrer,1999131513Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey,19901865316535Zietkiewicz, E., A. Rafalski and D. Labuda,199420176183Welsh, J. and M. McClelland,19901872137218Milbourne, D., R. Meyer, J.E. Bradshaw, E. Baird and N. Bonar et al.,19973127136Kumar, N., T. Jhang and T.R. Sharma,2011Colletotrichum falcatum infecting subtropical Indian sugarcane.]]>159260267Tymon, A.M. and J.K. Pell,2005Pandora neoaphidis.]]>109285293Mirmajlessi, S.M., N. Safaie, H.A. Mostafavi, M. Mansouripour and S.B. Mahmoudy,2012Rhizoctonia solani isolated from cucurbits using PCR based techniques.]]>7583590Archana, S., K. Prabakar and T. Raguchander,2014Colletotrichum gloeosporioides (Penz.) Penz. and evaluation of varietal susceptibility against mango anthracnose.]]>7415421Knapp, J.E. and J.M. Chandlee,1996215456Anderson, J.A., G.A. Churchill, J.E. Autrique, S.D. Tanksley and M.E. Sorrells,199336181186Mariette, S., V.L. Corre, F. Austerlitz and A. Kremer,20021111451156Rohlf, F.J.,19901st Edn.,Agwanda, C.O, P. Lashermes, P. Trouslot, M.C. Combes and A. Charrier,1997Colletotrichum kahawae, in Arabica coffee.]]>97241248Weeds, P.L., S. Chakraborty, C.D. Fernandes, M.J.D.A. Charchar, C.R. Ramesh, Y. Kexian and S. Kelemu,2003Colletotrichum gloeosporioides from Stylosanthes spp. at centers of origin and utilization.]]>93176185Lu, G., P.F. Cannon, A. Reid and C.M. Simmons,2004Colletotrichum isolates from the Iwokrama forest reserve, Guyana.]]>1085363Mahmodi, F., J.B. Kadir, A. Puteh, S.S. Pourdad, A. Nasehi and N. Soleimani,2014Colletotrichum spp. isolates associated with Leguminosae using multigene loci, RAPD and ISSR.]]>301024Abadio, A.K.R., S.S. Lima, M.F. Santana, T.M.F. Salomao and A. Sartorato et al.,2012Pseudocercospora griseola from central and southern Brazil.]]>1112721279Chadha, S. and T. Gopalakrishna,2007Magnaporthe grisea.]]>93688692Chakravarthi, B.K. and R. Naravaneni,2006Oryza sativa L.).]]>5684688Seehalak, W., N. Tomooka, A. Waranyuwat, P. Thipyapong, P. Laosuwan, A. Kaga and D.A. Vaughan,2006Vigna germplasm from Thailand and neighboring regions revealed by AFLP analysis.]]>5310431059Reddy, M.P., N. Sarla and E.A. Siddiq,2002128917Sharma, P.N., M. Kaur, O.P. Sharma, P. Sharma and A. Pathania,2005Colletotrichum capsici, the cause of fruit rot of chillies in the subtropical region of North-Western India.]]>153232237Ajibade, S.R., N.F. Weeden and S.M. Chite,2000Vigna.]]>1114755