Asian Journal of Plant Pathology1819-1541xxxx-xxxxScience International10.3923/ajppaj.2013.60.73Glomus Species in Egyptian Soil Using Fatty
Acid Methyl Ester Profiles]]>SabetK.K. MansourM.S. El-HadadS.A. M. ShaltoutAbeer E. El-AbeidSozan 2201372The present study was to investigate the variation among Glomus species.
Standard method used for Extraction, purification of Vesicular Arbuscular Mycorrhizal
(VAM) fungi, during summer and winter season. Study Effect of some factors on
number of spores collected from the rhizosphere of various plants in Egypt.
Used single spores technique inoculated on maize root to purified and identified
isolates of Glomus spp. The highest number of spores was found in summer
season while the lowest number was found in winter season. Variation of Glomus
spp. isolates among polymorphic DNA markers was studied. Species 2, 3, 4
and 7 identified by VANS1/VALETC primer as Glomus etunicatum. Fatty Acid
Methyl Ester (FAME) profiles were analyzed to assess the diversity and quantity
of fatty acids in 8 isolates of Glomus species. Spores and endomycorrhizal
roots of Sudan grass (Sorghum sudanense) were examined. Analysis of lipid
for species found that affinity specific and generic levels. Differentiation
on the genus level using lipid profiles and content which was carried out by
evaluating the methyl esters of fatty acids, revealed convergence between studied
isolates. Palmitic acid (16:0), Oleic acid (cis18:1n9) and Stearic acid (18:0)
were the dominant fatty acid of the Glomus spp., while the dominant fatty
acids of Glomus etunicatum isolates were myristic acid (14:0) palmitoleic
acid (16:1n7 cis), archidic acid (20:0), palmitic acid (16:0), oleic
acid (cis18:1n9) and stearic acid (18:0). This study indicates that using lipids
as biochemical markers to identify the different genus of AM fungi and differentiate
between the species.]]>Beilby, J.P.,1980Acaulospora laevis.]]>15949952Beilby, J.P. and D.K. Kidby,1980Glomus caledonius: Changes in neutral and polar lipids.]]>21739750Bentivenga, S.P. and J.B. Morton,19949814191426Bentivenga, P.S. and J.B. Morton,19969356595662Bever, J.D., P.A. Schultz, A. Pringle and J.B. Morton,200151923931Camargo-Ricalde, S.L. and M. Esperon-Rodriguez,200553339352Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Di Bonito, R., M. Elliot and E. Jarclin,19956128092810Fares, C.N.,19861986Gemma, J.N., R.E. Koske and M. Carreiro,198992317321Gerdemann, J.W. and T.H. Nicolson,196355158161Gerdemann, J.W. and J.M. Trappe,197457676Graham, J.H., N.C. Hodge and J.B. Morton,1995615864Hayman, D.S.,19821982pp: 401-442pp: 401-442Hedley, M.J. and J.W.B. Stewart,198214377385Henson, J.M., T. Goins, W. Grey, D.E. Mathre and M.L. Elliott,1993Geaumannomyces graminis DNA in plants grown in artificially and naturally infested soil.]]>83283287Hirrel, M.C.,1981Gigaspora margaria.]]>43610617Jansa, J., M. Gryndler and M. Matucha,199926247264Jabaji-Hare, S.,198880622629Johnson, N.C., P.J. Copeland, R.K. Crookston and F.L. Pfleger,199284387390Juniper, S. and L. Abbott,199344557Koske, R.E.,198176411416Lee, P.J. and R.E. Koske,1994Gigaspora gigantea: Seasonal abundance and ageing of spores in a sand dune.]]>98453457Lugo, M.A., A.M. Anton and M.N. Cabello,2005Larrea divaricata scrubland of the arid Chaco, Central Argentina.]]>1163178Madan, R., C. Parkhurst, B. Hawke and S. Smith,200234125128Morton, J.B.,1985Glomus occultum and Glomus diaphanum as influenced by plant host and soil environment.]]>77192200Negrin, M.A., M. Espino-Mesa and J.M. Hernandez-Moreno,199647385393Pacovsky, R.S.,1989Zea-glomus-azospirillum symbioses.]]>21953960Pacovsky, R.S. and G. Fuller,1988Glycine-glomus-bradyrhizobium symbioses.]]>72733746Peng, S., D.M. Eissenstat, J.H. Graham, K. Williams and N.C. Hodge,199310110631071Phillips, J.M. and D.S. Hayman,197055158161Sakamoto, K., T. Iijima and R. Higuchi,2004Gigaspora rosea.]]>3618271834Sanders, F.E., P.B. Tinker, R.L.B. Blacfk and S.M. Palmerley,197778257268Schoenau, J.J. and W.Z. Huang,199122465492Schultz, P.A.,19961996Schultz, P.A., J.D. Bever and J. Morton,1999Acaulospora colossica sp. nov. from an old field in North Carolina and morphological comparisons with similar species, A. laevis and A. koskei.]]>91676683Snedecor, G.W. and W.G. Cochran,19827th Edn.,Simon, L.,199613395101Simon, L. and M. Lalonde,19951995Simon, L., M. Lalonde and T. Bruns,199258291295Simon, L., R.C. Levesque and M. Lalonde,19935942114215Smith, S.E. and D.J. Read,1997Pages: 587Pages: 587Trappe, J.M.,1982Zygomycetous mycorrhizal fungi.]]>7211021108Trepanier, M., G. Becard, P. Moutoglis, C. Willemot, S. Gagne, T.J. Avis and J.A. Rioux,20057153415347Tresner, H.D. and J.A. Hayes,197122210213Tunlid, A. and D.C. White,19921992pp: 229-262pp: 229-262Walker, C. and J.M. Trappe,199397339344Williams, K., F. Percival, J. Merino and H.A. Mooney,198710725734Wyss, P. and P. Bonfante,19939713511357Yayli, N., Z. Kiran, H. Seymen, H. Genc and M. Kucukislamoglu,2001Crocus vallicola.]]>25391396