American Journal of Food Technology1557-45711557-458XAcademic Journals Inc.10.3923/ajft.2011.647.660Coelho SilvestreMarialice PintoAfonsoWendel de OliveiraLopes JuniorCarlos de OliveiraMedeiros SilvaViviane DiasMoraisHarriman Aleyde SouzaMariana Wanessa SantanaSilvaMauro Ramalho8201168The objective this study was to evaluate the preparation of protein hydrolysates with an appropriate peptide profile as well as the reduction of costs for scaling-up the process, the actions of subtilisin and pancreatin on Whey Protein Concentrate (WPC) were tested. The effect of Enzyme: Substrate ratio (E:S) and Substrate Concentration (SC) was also verified. The hydrolysates were fractionated by size-exclusion-HPLC and the rapid correct fraction area method was used for quantifying peptides and free amino acids. The best peptide profiles were very similar giving rise to 13.17% of di-tripeptides, 46.41% of free amino acids and 12.31% of large peptides on an average. The advantage of using a higher SC was observed only in one case and for the pancretin action. The use of a smaller E:S ratio for subtilisin action was advantageous only when comparing 2:100 with 4:100 while for pancretin this beneficial result was also achieved when comparing either 1:100 with 2:100 or 2:100 with 4:100. It can be inferred that the use of these two enzymes was able to produce some WPC hydrolysates with appropriate peptide profiles for dietary purposes.]]>Adachi, S., Y. Kimura, K. Murakami, R. Matsuno and H. Yokogoshi,199155925932Akiyama, H., K. Sakata, Y. Yoshika, Y. Murata and Y. Ishihara et al.,20061403642Amiot, J. and G.J. Brisson,1980193496499AOAC.,199516th Edn.,Pages: 1298Pages: 1298 Armstead, I.P. and J.R. Ling,1991586259263Aubry, A.F., M. Caude and R. Rosset,199233533538De Araujo, R.L.B.,20092009Biasutti, E.A.R., C.R. Vieira, M. Capobiango, V.D.M. Silva and M.P.C. Silvestre,200710173183Biasutti, E.A., W.O. Afonso, C.O. Lopes Jr., J.V. Coelho, V.D.M. Silva and M.P.C. Silvestre,2008445160Bizzotto, C.S., M. Capobiango and M.P.C. Silvestre,200541116Boza, J.J., D. Moennoz, J. Vuichoud, A.R. Jarret, D. Gaudard-de-Weck and O. Ballevre,200039237243Brans, G., C.G.P.H. Schroen, R.G.M. van der Sman and R.M. Boom,2004243263272Capobiango, M., C.S. Bizzotto, E.A.R. Biasutti and M.P.C. Silvestre,20069357364Carreira, R.L., L.M. De Marco, D.R. Dias, H.A. Morais and M.P.C. Silvestre,2004231726Chicon, R., J. Belloque, E. Alonso and R. Lopez-Fandino,200923593599Clemente, A.,200011254262Contreras, M.D.M., B. Hernandez-Ledesma, L. Amigo, P.J. Martin-Alvarez and I. Recio,201044915Davis, M.T. and T.D. Lee,19921935944Deeslie, W.D. and M. Cheryan,1988362631Delvivo, F.M., C.R. Vieira, E.A.R. Biasutti, M. Capobiango, V.D.M. Silva, W.O. Afonso and M.P.C. Silvestre,2006194104Frenhani, P.B. and R.B. Burini,199936227237Golovchenko, N.P., I.A. Kataeva and V.K. Akimenko,1992591121128Gonzalez-Tello, P., F. Camacho, E. Jurado, M.P. Paez and E.M. Guadix,199444529532Grimble, G.K., P.P. Keohane, B.E. Higgins, M.V. Kaminski Jr. and D.B. Silk,1986716569Gupta, R., Q. Beg and P. Lorenz,2002591532Kammoun, R., S. Bejar and R. Ellouz,200390249254Keohane, P.P., G.K. Grimble, B. Brown, R.C. Spiller and D.B. Silk,198526907913Kong, X., H. Zhou and H. Qian,2007102759763Kopaciewicz, W. and F.E. Regnier,1982126816Lemieux, L. and J. Amiot,1989473189206Lemieux, L., J.M. Piot, D. Guillochon and J. Amiot,199132499504Li-Jun, L., Z. Chuan-He and Z. Zheng,20088616Lopes, D.C.F., C.S. Bizzotto, R.L. Carreira, W.O. Afonso, C.O. Lopes Jr. and M.P.C. Silvestre,200865765Lopes, D.C.F., F.M. Delvivo and M.P.C. Silvestre,20051074253Morais, H.A., L.M. De Marco, M.C. Oliveira and M.P.C. Silvestre,2005345969Morato, A.F., R.L. Carreira, R.G. Junqueira and M.P.C. Silvestre,200013843857Moreno-Indias, I., N. Castro, A. Morales-delaNuez, D. Sanchez-Macias, P. Assuncao, J. Capote and A. Arguello,20099247924796Mortenson, M.A., Z.M. Vickers and G.A. Reineccius,200818649657Nielsen, S.S.,19982nd Edn.pp: 630pp: 630Page, M.J. and E. Di Cera,20086512201236Park, Y.K.,20012001pp: 363-371pp: 363-371Parrado, J., F. Millan, I. Hernandez-Pinzan, J. Bautista and A. Machado,19934118211825Peng, X., Y.L. Xiong and B. Kong,2009113196201Perea, A., U. Ugalde, I. Rodriguez and J.L. Serra,199315418423Pimentel-Gomes, F.,200014th Edn.,Pouliot, Y., M.M. Guy, M. Tremblay, A.C. Gaonach, B.P.C.P. Ting, S.F. Gauthier and N. Voyer,20095737603764Rerat, A.A.,199352335344Sammel, L.M. and J.R. Claus,20036512931299Saint-Sauveur, D., S.F. Gauthier, Y. Boutin and A. Montoni,200818260270Schmidt, C.G. and M. Salas-Mellado,20093211441150Schmidt, D.G. and J.K. Poll,199145225240Silva, V.D.M., L.M. De Marco, W.O. Afonso, D.C.F. Lopes and J.N. Januario et al.,20072327341Silva, M.C.D., V.D.M. Silva, A.M.Q. Lana and M.P.C. Silvestre,200920395402Silva, M.R.,20102010Silva, V.D.M. and M.P.C. Silvestre,200336709718Silvestre, M.P.C., M. Hamon and M. Yvon,19944227782782Silvestre, M.P.C., M. Hamon and M. Yvon,19944227832789De Souza, M.W.S., E.A.R. Biasutti, R.L. Carreira, W.O. Afonso, V.D.M. Silva and M.P.C. Silvestre,20083315324Su, R., Z. He and W. Qi,2008107151157Vallejo-Cordoba, B., S. Nakai, W.D. Powrie and T. Beveridge,19865111561161Visser, S., C.J. Slagen and A.J.P.M. Robben,1992599205209Voorbergen, M. and A. Zwanenberg,2002672528Walzem, R.L., C.J. Dillard and J.B. German,200242353375Wang, J., M. Zhao and Y. Jiang,200745410415