American Journal of Food Technology1557-45711557-458XAcademic Journals Inc.10.3923/ajft.2007.457.461ShahidMuhammad Akbar SiddiqueMuhammad Sajjad-Ur-Rehman HameedSajid HussainAbid 5200725The present study was conducted to monitor the presence of antibiotic residues in poultry meat. Swab Test on Animal Food (STAF) employing Bacillus subtilis as test organism on nutrient agar with 0.4% dextrose at pH 7, was used. A spore suspension of 2x107 spores mL-1 was used in 100 mL of nutrient agar to make STAF plates. A total of 100 tissue samples (33 liver, 33 kidney and 34 muscles) were collected from local market of Rawalpindi and Islamabad. Thirteen (39.4%) liver, 9 (27.3%) kidney and 7 (20.6%) muscle samples were detected positive for antibiotic residues. Antibiotic sensitivity and Minimum Inhibitory Concentration (MIC) of Oxytetracycline, Gentamicin, Neomycin, Enrofloxacin, Chloramphenicol, Amoxicillin, Benzyl Penicillin, Streptomycin and Tylosin against Bacillus subtilis was determined to be 0.6100, 0.0549, 0.1525, 0.1525, 0.0023, 0.0143, 0.0095, 0.0380 and 0.0023 μg, respectively. It is concluded from the study that STAF test can be used for the preliminary detection of antimicrobial residues in poultry tissues.]]>Awan, J.A. and S.U. Rahman,20022002Booth, N.H.,19881988pp: 1156pp: 1156Carlisle, G.E. and J.O. Falkinham,1989Bacillus subtilis and Bacillus licheniformis.]]>5530263028Cheesbrough, M.,1989Vol. 11,Choi, L., D.G. Lindsay and D.H. Watson,19995911221124Cruickshank, R., J.P. Duguid, B.P. Marmion and R.H.A. Swain,197512th Edn.,Pages: 587Pages: 587Donoghue, D.J.,200382618621De Wasch, K., L. Okerman, H. De Brabander, J. Van Hoof, S. Croubels and P. De Backer,199812327372741Economic Survey of Pakistan,2004-20052004-2005FDA, 19961st Edn.,Hrdlicka, J.,199035411418Jabbar, A.,20042004Korsrud, G.O., J.O. Boison, J.F.M. Nouws and J.D. MacNeil,1998812124Kozarova, I., D. Mate and R. Cabadaj,20012001pp: 159163Myllyniemi, A.L., R. Rannikko, E. Lindfors, A. Niemiand and C. Backman,2000179911001Popelka, P., J. Nagy, R. Germuška, S. Marcinčák, P. Jevinová and A. de Rijk,200522557562Rehman, S.U. and A. Jabbar,20062006Tortora, G.J., B.R. Funke and C.L. Case,20017th Edn.,