American Journal of Food Technology1557-45711557-458XAcademic Journals Inc.10.3923/ajft.2007.30.37 Aeromonas sp.from Chicken and Fish Samples by Polymerase Chain Reaction]]>PorteenK. AgarwalR.K. BhilegaonkarK.N. 1200721A polymerase chain reaction was standardized for the identification of Aeromonas sp. using primers against 16S rRNA gene and aerolysin gene. The primers used were found to be highly specific for Aeromonas sp. and did not give positive result with other bacteria including other Gram positive and Gram-negative bacteria. The minimum detection level of PCR was found to be 102 and 104 cells mL-1 in case of 16S rRNA and aerolysin gene targeted assay, respectively. Suitability of the enrichment broth (Alkaline peptone water-cephalothin, APW-C) when tested to detect Aeromonas from the spiked samples gave good results on direct usage of the broth for template preparation without any subsequent treatment. The kinetics of the spiking study indicated that a minimum of 24 h enrichment was required for the detection of Aeromonas by cultural and PCR method. Among two PCR assays detection limits achieved by PCR targeting16S rRNA gene were better than aerolysin gene PCR assay. The results were comparable to cultural method. A total of 100 samples comprising of 50 each of chicken and fish samples were screened by cultural and PCR methods for the presence of Aeromonas. Two chicken samples and three fish samples turned out to be positive by both cultural method and PCR targeting 16S rRNA. From this study it was concluded that PCR assay targeting 16S rRNA gene can be used for the rapid detection of Aeromonas from chicken and fish samples after one step enrichment in APW-C.]]>Agarwal, R.K., K.N. Kapoor, A. Kumar and K.N. Bhilegaonkar,200070942943Bachhil, V.N., K.N. Bhilegaonkar and R.K. Agarwal,2002Aeromonas sp. in meat and milk.]]>238182De Boer, E. and R.R. Beumer,199950119130Gonzalez-Rodriguez, M.N., J.A. Santos, A. Otero and M.L. Garcia-Lopez,200293675680Gonzalez-Villasenor, L.I. and M.M. Manak,19981998pp: 579pp: 579Graf, J.,1999Aeromonas veronii strains and possible misidentification of Aeromonas sp.]]>3731943197International Commission on Microbiological Specifications for Food,1996pp: 5-19pp: 5-19Janda, J.M. and S.L. Abbott,1998Aeromonas: An expanding panorama of species, disease presentations and unanswered questions.]]>27332344Khan, A.A. and Cerniglia,1997Aeromonas caviae and Aeromonas trota by polymerase chain reaction.]]>24233239Khurana, R. and A. Kumar,199734228229Lee, H.A., G.M. Wyatt, S. Bramham and M.R.A. Morgan,1990Salmonella typhimurium in food: Feasibility 1-day Salmonella detection.]]>5615411546Olsen, J.E.,200033257266Rahman, M.,20022002Rossen, L., P. Norskov, K. Holmstrom and O.F. Rasmussen,1992173745Sachan, N. and R.K. Agarwal,2000Aeromonas sp. From ckicken meat.]]>606574Santos, J.A., C.J. Gonzalez, A. Otero and M. Garcia-Lopez,1999Aeromonas sp. isolated from fish.]]>6556125614Surendran, D., R.K. Agarwal and K.N. Bhilegaonkar,2003Salmonella from meat.]]>1113124Wang, G., K.D. Tyler, C.K. Murno and W.M. Johnson,1997Aeromonas caviae clinical isolates and their identification by determining presence of a unique haemolysin gene.]]>3432033205