Asian Journal of Clinical Nutrition1992-14702077-2033Asian Network for Scientific Information10.3923/ajcn.2014.1.17Ali HusseinSamy El-Said AzabMohammed Kamal El-ShallSoheir 1201461Iron overload (IOL) is one of the most common metal related toxicity. Under this circumstance, excessive iron deposition in liver will lead to further injury such as hepatocellular necrosis, inflammation, fibrosis and even to carcinoma. In this study, the effect of a nature flavonoid, curcumin, on IOL-induced rats liver oxidative damage was investigated. Sixty male albino rats were randomly divided into three equal groups. Group I: Control group, Group II: Iron-overload group, received six doses (three doses per week) of ferric hydroxide poly maltose (100 mg kg-1 b.wt.) by intraperitoneal injections (IP) and Group III: Iron overload+curcumin group, received six doses of 100 mg kg-1 b.wt. ferric hydroxide poly maltose (IP) and curcumin (100 mg kg-1 b.wt.) as one daily oral dose until the sacrificed day. Blood samples for serum separation and liver tissue specimens were collected three times, after three, four and five weeks from the onset of the experimental. Serum iron profile (Iron, Total iron Binding Capacity (TIBC), Unsaturated Iron Binding Capacity (UIBC), transferrin (Tf) and transferrin saturation% (TS%)}, ferritin, albumin, total protein, total cholesterol and triacylglycerols levels and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were determined. Moreover, iron, L-malondialdehyde (L-MDA), reduced glutathione (GSH), nitric oxide (NO) and total nucleic acid (TNA) levels and glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) activities in the liver were also determined. The obtained results revealed that, IOL resulted in significant increase in serum iron, TIBC, Tf, TS% and ferritin levels and AST and ALT activities and also increased liver iron, L-MDA and NO levels. 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