Asian Journal of Cell Biology1814-00682152-3517Academic Journals Inc.10.3923/ajcb.2017.20.27Kigelia pinnata Leaves]]>RaviLokesh KrishnanKannabiran 12017121Objective: The aim of the present study was to screen and to identify the cytotoxic compound from the leaves of Kigelia pinnata. Materials and Methods: Dry leaf powder of K. pinnata was extracted with chloroform and the extract was tested for anticancer cytotoxic activity by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide (MTT) assay in human colorectal carcinoma (HCT-116) cells. Crude extract was purified by silica gel column chromatography and the active fraction was identified by using spectroscopic techniques such as nuclear magnetic resonance, fourier transform-infrared spectroscopy and gas chromatography-mass spectrometry analysis. The identified compound was docked with cancer drug target protein, DNA topoisomerase-I using AutoDock4. Results: Crude extract showed significant anticancer cytotoxic activity with IC50 value of 80 μg mL1. The GC-MS chromatogram showed the presence of more than 15 different phytochemical compounds. The active fraction of the crude was identified as N-hexadecanoic acid with a molecular weight 256.42 Da and molecular formula C16H32O2. Molecular docking analysis showed that N-hexadecanoic acid has high affinity interaction with DNA topoisomerase-I with a free binding energy -6.71 kcal mol1. The N-hexadecanoic acid demonstrated significant IC50 value of 0.8 μg mL1 against HCT-116 cells. Conclusion: Based on the results of docking studies, it is proposed that the observed cytotoxic activity of N-hexadecanoic acid is due to its interaction with DNA topoisomerase-I and itcould be explored further for its anticancer cytotoxic potential with other cancer drug target proteins.]]>Olatunji, A.G. and O. Atolani,2009Kigelia africana: A review.]]>3158164Atawodi, S.E.O. and O.D. Olowoniyi,2015Kigelia africana (Lam.) Benth.]]>5117Siddiqui, K., A. Mazumder and G. Chakraborthy,2015Kigelia pinnata (Jacq.).]]>43438Picerno, P., G. Autore, S. Marzocco, M. Meloni, R. Sanogo and R.P. Aquino,2005Kigelia africana and evaluation of cutaneous irritation in cell cultures and reconstituted human epidermis.]]>6816101614Kumari, P., R. Sachan, P. Yadav, P. Tomer, A. Arya, S. Tripathi and S. Kumar,2012in vitro and in vivo Kigelia pinnata (Jacq.) DC from Indian origin.]]>2261268Saini, S., H. Kaur, B. Verma, Ripudaman and S.K. Singh,2009Kigelia africana (Lam.) Benth.-An overview.]]>8190197Sharma, U.K., A. Singh, U. Sharma, M. Kumar, D. Rai and P. Agrahari,2010Kigelia pinnata bark extract.]]>37375Arkhipov, A., J. Sirdaarta, P. Rayan, P.A. McDonnell and I.E. Cock,2014Kigelia africana fruit extracts.]]>46276Rustan, A.C. and C.A. Drevon,20052005pp: 1-7pp: 1-7Harada, H., U. Yamashita, H. Kurihara, F. Fukushi, J. Kawabata and Y. Kamei,20022225872590Rashmi, A. Singh, A. Jain, P. Kaushal and Sangeeta et al.,2012Kalanchoe pinnata against pathogens.]]>550625063Guo, J., U.N. Verma, D. Tripathy, E. Pfrenkel and C.R. Becerra,20062713751381Davoodi, H., S.R. Hashemi and H.F. Seow,201312453460Mondal, S., S. Majumdar, V. Geetha, G. Gadamsetty, R. Lakshmipathy, A. Sheela and N.C. Sarada,2014Drypetes sepiaria.]]>1207213Suneetha, S.C.A., B.P.C. Raghupathy and P.K. Suresh,201482857872Badrinathan, S., T.M. Shiju, A.S.S. Christa, R. Arya and V. Pragasam,2012Sargassum myriocystum and its efficacy in scavenging free radicals.]]>74549555Chivandi, E., E. Cave, B.C. Davidson, K.H. Erlwanger, D. Moyo and M.T. Madziva,2012Kigelia africana, Mimusops zeyheri and Ximenia caffra seed oils.]]>2699105Fouche, G., G.M. Cragg, P. Pillay, N. Kolesnikova, V.J. Maharaj and J. Senabe,2008In vitro anticancer screening of South African plants.]]>119455561Atolani, O., G.A. Olatunji, O.A. Fabiyi, A.J. Adeniji and O.O. Ogbole,2013Kigelia pinnata leaves show antioxidant and anticancer potential on human cancer cell line.]]>16878885Atolani, O., S. Oladoye, O.S. Adeyemi and G.A. Olatunji,2014In vitro antiproliferative and antimicrobial evaluation of Kigelia pinnata.]]>122932Higgins, C.A., T. Bell, Z. Delbederi, S. Feutren-Burton and B. McClean et al.,2010Kigelia pinnata.]]>7618401846Zofou, D., A.B.O. Kengne, M. Tene, M.N. Ngemenya, P. Tane and V.P. Titanji,2011In vitro antiplasmodial activity and cytotoxicity of crude extracts and compounds from the stem bark of Kigelia africana (Lam.) Benth (Bignoniaceae).]]>10813831390Houghton, P.J. and A.K. Jager,2002Kigelia pinnata): Ethnobotany and recent scientific work.]]>681420Jackson, S.J., P.J. Houghton, S. Retsas and A. Photiou,2000In vitro cytotoxicity of norviburtinal and isopinnatal from Kigelia pinnata against cancer cell lines.]]>66758761