American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2013.304.313Orthaga exvinacea Hampson]]>UmadeviD. RafeeqK.U.M.A. AjaykumarA.P. IsmailK.M. RasheedK.A. GokuldasM. 3201333Neuropeptides of the adipokinetic/red pigment-concentrating hormone family (AKH/RPCH) regulate energy metabolism in insects. The present study was carried out to elucidate the primary structure of an adipokinetic neuropeptide in the mango leaf webber, O. exvinacea employing bioassay, High Pressure Liquid Chromatography (HPLC) analysis and mass spectrometric studies. The presence of adipokinetic activity in the crude extracts of brain-retrocerebral complexes of O. exvinacea was demonstrated by heterologous in vivo bioassay in the polyphagous plant bug Iphita limbata. The extract was separated by Reverse Phase-High Pressure Liquid Chromatography (RP-HPLC) and the purified fractions were tested for their adipokinetic effects on the fat body. The maximum hyperlipemic activity was shown by fraction 10. MALDI-TOF-MS analysis of the fraction indicated that the molecular ion peak m/z value 1030.471 Da is indicative of sodiated adduct of peptide with mass 1008.471. This molecular ion peak has similar mass as that of already known AKH/RPCH peptide, Manduca adipokinetic hormone (Manduca AKH). Tandem MS/MS confirmed that the primary structure of O. exvinacea AKH is pE-L-T-F-T-S-S-W-G-NH2 which is identical with Manse-AKH.]]>Ajaykumar, A.P. and M. Gokuldas,2011 Iphita limbata stal.]]>1349358Ajaykumar, A.P. and M. Gokuldas,2011Aularches miliaris L. (Pyrgomorphidae: Orthoptera).]]>5127135Ajaykumar, A.P. and M. Gokuldas,2011Oryctes rhinoceros L (Coleoptera: Dynastidae).]]>18100104Beenakkers, A.M., D.J. Van der Horst and W.J.A. Van Marrewijk,1985241967Carlisle, J.A. and B.G. Loughton,1979Locusta.]]>282420421Frings, C.S., T.W. Fendley, R.T. Dunn and C.A. Queen,197218673674Gade, G.,199651607617Gade, G., Hoffmann, K.H. and J.H. Spring,1997779631032Gokuldas, M., A.P. Ajaykumar, D. Umadevi, V.S. Binitha, K.M. Ismail and K.A. Rasheed,2012Hieroglyphus banian (Acrididae: Orthoptera).]]>2012Gokuldas, M., P.A. Hunt and D.J. Candy,1988in vitro in the locust, Schistocerca gregaria, by factors from the corpora cardiaca.]]>134348Goldsworthy, G.J.,19831983pp: 149-204pp: 149-204Ishibashi, J., H. Kataoka, H. Nagasawa, A. Isogai and A. Suzuki,1992Bombyx mori.]]>566670Jaffe, H., A.K. Raina, C.T. Riley, B.A. Fraser and G.M. Holman et al.,1986Heliothis zea with adipokinetic activity.]]>135622628Jaffe, H., A.K. Raina, C.T. Riley, B.A. Fraser and T.G. Bird et al.,1988Heliothis zea with hypertrehalosemic and adipokinetic activities.]]>155344350Kodrik, D.,200833171180Kodrik, D. and G.J. Goldsworthy,1995Locusta migratoria.]]>41127133Kollisch, G.V., M.W. Lorenz, R. Kellner, P.D. Verhaert and K.H. Hoffman,2000Vanessa cardui.]]>26755025508Kumari, P.S. and M. Gokuldas,2001Spodoptera mauritia (Noctuidae: Lepidoptera) to neuropeptide hormones.]]>265364Lorenz, M.W.,200147198214Rasheed, K.A. and M. Gokuldas,2002Iphita limbata Stal. (Pyrrhocoridae: Heteroptera).]]>27125136Ziegler, R., K. Eckart, H. Schwarz and R. Keller,1985Manduca sexta adipokinetic hormone elucidated by combined Fast Atom Bombardment (FAB)/tandem mass spectrometry.]]>133337342Ziegler, R.,1995Manduca sexta: Physiological Actions.]]>1995pp: 35-41pp: 35-41