American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2011.223.230Triticum turgidum ssp. carthlicum) Accessions by EST-SSR Markers]]>ZhuangPing-ping RenQin-ce LiWei ChenGuo-Yue 2201112To estimate the allelic variation at the expressed sequence tag simple sequence repeats (EST-SSR) among Persian wheat (Triticum turgidum ssp. carthlicum L.) accessions collected from fifteen countries and provide information for wheat breeding and improvement in southwest China, 87 Persian wheat accessions from fifteen countries were investigated by using EST-SSR markers. EST-SSRs were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected by EST-SSRs might reflect better relationship among species or varieties. Fourteen primer pairs could successfully amplify the fragments in the 87 accessions, of which tri-nucleotide repeats were the dominant type. A total of 33 eSSR alleles were detected, and the number of alleles detected by single pair primers ranged from 3 to 7 per locus, with an average of 3.71. Clustering analysis suggested that most of the accessions with adjacent geographic origins had the tendency to cluster together. Therefore, when used in Persian wheat genetic analysis, EST-SSR markers not only act as genetic markers but also reveal differences in related gene expression.]]>Allard, R.W.,199692111Anamthawat-Jonsson, K.,19969101113Balatero, C.H. and N.L. Darvey,199266179185Belay, G., A. Merker and T. Esemma,1994Triticum turgidum L.) I. Spike morphology vs ploidy level and karyomorphology.]]>1214552Chambers, G.K. and E.S. MacAvoy,2000126455476Chen, H.M., L.Z. Li, X.Y. Wei, S.S. Li and T.D. Lei et al.,20055023282336Dong, Y.C. and Z. Dian-Sheng,1998Ellegren, H.,20045435445Eujayl, I., M.E. Sorrells, M. Baum, P. Wolters and W. Powell,2002104399407Fahima, T., M.S. Roder, K. Wendehake, V.M. Kirzhner and E. Nevo,2002Triticum dicoccoides, in Israel.]]>1041729Fahima, T., G.L. Sun, A. Beharav, T. Krugman, A. Beiles and E. Nevo,1999Triticum dicoccoides, in Israel.]]>98434447Fu, Y.B., G.W. Peterson, K.W. Richards, D. Somers, R.M. De Pauw and J.M. Clarke,200511015051516De Moraes Fernandes, M.I.B., A.C.A. Zanatta, A.M. Prestes, V. da Rosa Caetano, A.L. Barcellos, D.C. Angra and V. Pandolfi,2000Triticum aestivum L. em. Thell).]]>2310511062Gol-Denberg, Z.V.,1984114876878Hoisington, D., M. Khairallah, T. Reeves, J.M. Ribaut, B. Skovmand, S. Taba and M. Warburton,19999659375943Maccaferri, M., M.C. Sanguineti, P. Donini and R. Tuberosa,2003107783797Mantel, N.,196727209220Merker, A. and K. Lantai,1997Leymus and Thinopyrum species.]]>474851Nevo, E., E. Golenberg, A. Beiles, A.H.D. Brown and D. Zohary,1982Triticum dicoccoides, in Israel.]]>62241254Nicot, N., V. Chiquet, B. Gandon, L. Amilhat and F. Legeai et al.,2004109800805Pandey, H.N. and M.V. Rao,1987Triticum durum through interspecific hybridization.]]>47133135Plaschke, J., M.W. Ganal and M.S. Roder,19959110011007Peng, J.H. and N.L.V. Lapitan,200558096De Pienaar, R.V. and E.R. Sears,19731973pp: 253258Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski,19962225238Raut, V.M., V.P. Patil and G.B. Deodikar,198410101106Rohlf, F.J.,1990pp: 28-45pp: 28-45Sharp, P.J., M. Kresis, P.R. Shewry and M.D. Gale,198875286290Tixier, M.H., P. Sourdille, M. Roder, P. Leroy and M. Bernard,199751175177Wang, H.Y., Y.M. Wei, Z.H. Yan and Y.L. Zheng,2007Triticum durum L.) collections.]]>483542Yang, X.Q., P. Liu, Z.F. Han, Z.F. Ni, W.Q. Liu and Q.X. Sun,2005Triticum asetivum L.).]]>32406416, (In Chinese)Yeh, F.C. and T.J.B. Boyle,1997129157163Yifru, T., K. Hammer, X.Q. Huang and M.S. Roder,2006125125130Zhuang, P.P., Z.Q. Zhang and Y.L. Zheng,2005Triticum carthlicum L.]]>259297Zhuang, P.P., J.R. Wang, Y.M. Wei and Y.L. Zheng,2007Triticum carthlicum Nevski).]]>2528536