Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2011.208.2132 from the Erythrocytic Stage of Plasmodium berghei]]>IbrahimM.A. SallauA.B. SalihuA. OnwubeK.C. 2201162Phospholipase A2 (EC. 3.1.1.4) was isolated and partially characterized from the erythrocytic stage of Plasmodium berghei (NK-65) obtained from experimentally infected mice with the objective of studying its kinetic properties and its possible role in the pathogenesis of malaria. The parasite collected by sucrose gradient centrifugation was subjected to lysis to obtain the crude phospholipase A2 which was assayed and subjected to some biochemical characterizations . The enzyme had broad pH and temperature ranges with optima of 7.5 and 37°C, respectively. Initial velocity studies for the determination of kinetic parameters with L-α lecithin as substrate revealed a KM and VMAX of 0.68 mg mL-1 and 52.60 μmol min-1, respectively. The P. berghei PLA2 was slightly activated by Ca2+ while Cu2+, Zn2+and Mn2+ were inhibitory to the enzyme. However, no enzyme activity was detected in the presence of Mg2+ and Hg2+. Considering the findings of this work, P. berghei can be said to contain PLA2 which has similar properties with some other parasites PLA2 and could be involved in cellular invasion and/or aneamia development during cerebral malaria.]]>Bhat, M.K. and T.V. Gowda,19892 from Indian cobra (Naja naja naja) venom.]]>27861873Cassaing, S., J. Fauvel, H. Bessieres, S. Guy, J. Seguela and H. Chap,2000Toxoplasma gondii secretes calcium independent phospholipase A2.]]>3011371142Deregnacourt, C. and J. Schrevel,2000Plasmodium falciparum.]]>2753997339980Ecker, A., S.B. Pinto, K.W. Baker, F.C. Kafatos and R.E. Sinden,2007Plasmodium berghei: Plasmodium perforin-like protein 5 is required for mosquito midgut invasion in Anopheles stephensi.]]>116504508Fernandez, V., C.J. Treutiger, G.B. Nash and M. Wahlgren,1998Plasmodium falciparum malaria.]]>6629692975Jones, T.R., D.F. Stroncek, A.S. Gozalo, N. Obaldia and E.M. Andersen et al.,2002Plasmodium falciparum.]]>66672679Joshi, P., G.P. Dutta and C.M. Gupta,1987Plasmodium knowlesi) induces stage-dependent alterations in membrane phospholipid organization of its host erythrocyte.]]>246103108Kohler, G.A., A. Brenot, H. Haas-Stapleton, N. Agabian, R. Deva and S. Nigam,2006176113911399Lubick, K.J. and D.E. Burgess,2004Trichomonas vaginalis.]]>7212841290Margarida, C., P. Silvia, F. Maria and M.M. Maria,2006Plasmodium berghei ANKA are not affected in macrophage scavenger receptors a deficient mice.]]>615Nok, A.J., K.A.N. Esievo, S. Ibrahim, A.I. Ukoha and C.O. Ikediobi,1993Trypanosoma congolense: Characterization and heamatological properties.]]>11125130Zidovetzki, R., I.W. Sherman and L. O'Brien,1993Plasmodium falciparum phospholipase A2 by chloroquine, quinine and arteether.]]>79565570Sallau, A.B., M.A. Ibrahim, A. Salihu and F.U. Patrick,2008Echis ocellatus venom.]]>298101Iyawe, H.O.T. and A.O. Onigbinde, 2009Plasmodium berghei and chloroquine on heamatological and antioxidant indices in mice.]]>43035Iyawe, H.O.T., A.O. Onigbinde and O.O. Aina,2006Plasmodium berghei infested mice.]]>214