Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2010.145.153Glycine max)]]>BashirH. KhanT. MasoodA. HamidR. 3201053A purification scheme was developed to purify a lectin from the seeds of a local Kashmiri variety of Glycine max (soybean). The lectin that specifically binds to N- acetyl galactosamine was purified to electrophoretic homogeneity by affinity chromatography on CNBr activated Sepharose 6B column. Human blood group A, B, O and AB erythrocytes were used for agglutination assay and the sugar specificity was determined by hemagglutination inhibition assay. Protein estimation was done by Lowrys method and analysis was done by PAGE both under native conditions and in presence of SDS. The purified soybean lectin (SBL) showed equal agglutination with all four types of blood groups i.e., A, B, O and AB. Hemagglutinating activity of the lectin is inhibited by N- acetyl galactosamine and galactose and other carbohydrates containing the galactopyranosyl residue. The purified lectin gave a single symmetric protein peak on gel filtration chromatography showing a molecular weight of 110 kDa and when subjected to native PAGE, showed a single protein band. A single band of 30 kDa was obtained upon SDS-PAGE, establishing that the lectin is composed of similar subunits i.e., it is a homotetramer.A tetrameric galactose specific lectin that shows equal activity with all blood type human erythrocytes was purified and characterized from a Kashmiri variety of soybean.]]>Diaz, C.L., L.S. Melchers, P.J.J. Hooykaas, B.J.J. Lugtenberg and J.W. Kijne,1989Rhizobium-legume symbiosis.]]>338579581Entlicher, E.G., V.V. Kostır and J. Kocourek,1970Pisum sativum L.).]]>221272281De Mejia, E.G. and V.I. Prisecaru,200545425445Franco-Fraguas, L., A. Pla, F. Ferreira, H.M.N. Suarez and F. Batista-Viera,2003790365372Gorocica, P., R. Lascurain, P. Hemandez, F. Porras, S. Bouquelet, L. Vazquez and E. Zenteno,1998Amaranthus leucocarpus lectin from marine peritoneal macrophages.]]>15809814Hsu, K.L., K.T. Pilobello and L.K. Mahal,20062153157Laemmli, U.K.,1970227680685Lis, H., C.N. Fridman, N. Sharon and E. Katchalski,1966117301309Lis, H. and N. Sharon,1973Phytohemagglutinins).]]>42541574Lis, H. and N. Sharon,1986553567Lis, H. and N. Sharon,199898637674Loris, R., T. Hamelryck, J. Bouckaert and L. Wyns,19981383936Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Naeem, A., R.H. Khan, H. Vikram and M. Akif,2001Cajanus cajan root lectin and its interaction with rhizobial lipopolysaccharide as studied by different spectroscopic techniques.]]>39699105Peumans, W.J., B. Peters and B.P. Cammue,1985227949995Peumans, W.J. and E.J.M.V. Damme,1995109347352Peumans, W.J., A. Barre, H. Qiang, P. Rouge and E.J.M. van Damme,20001283101Quinn, J., M. Etzler and E. Marilynn,1987Dolichos biflorus.]]>258535544Reisner, Y., L. Itzicovitch, A. Meshorer and N. Sharon,19787529332936Reisner, Y., N. Kapoor, D. Kirkpatrick, M.S. Pollack and S. Cunningham-Rundles et al.,198361341348Rosenfeld, R., H. Bangio, G.J. Gerwig, R. Rosenberg and R. Aloni et al.,200770415426Rudiger, H., H.S. Siebert, D. Solis, J.J. Barbero and A. Romero et al.,20007389416Saez, F.J., J.F. Madrid, R. Aparicio, O. Leis and B. Oporto,1999Pleurodeles waltl.]]>16639648Sela, B.A., H. Lis, N. Sharon and L. Sachs,19703267279Sharon, N. and H. Lis,1989246227234Sharon, N. and H. Lis,200414R53R62Spilatro, S.R. and J.M. Anderson,19899013871393Tateno, H., N. Uchiyama, A. Kuno, A. Togayachi, T. Sato, H. Narimatsu and J. Hirabayashi,20071711381146Van Damme, E.J.M., W.J. Peumans, A. Pusztai and S. Bardocz,1998pp: 31-50pp: 31-50Weis, W.I. and K. Drickamer,199665441473Wu, A.M., A. Herp, S.C. Song, J.H. Wu and, K.S.S. Chang,19955718411852