Mohammad Abyari
Department of Biology, Faculty of Science, Urmia University, Iran
Reza Heidari
Department of Biology, Faculty of Science, Urmia University, Iran
Rashid Jamei
Department of Biology, Faculty of Science, Urmia University, Iran
ABSTRACT
Neutral solution of isolated anthocyanins are unstable and quickly decolourized by reactions. Thus anthocyanins in living cells may have mechanisms for resistance to maintain colour stability. Copigmentation is the main colour-stabilizing mechanism. In this study copigmentation of Siahe Sardasht grape anthocyanin were investigated with five copigment, tannic acid, coumaric acid, ferulic acid, benzoic acid and caffeic acid. In this research, the dependence of the copigmenation process on the pH, copigment concentration and temperature were established. Also the effects of uv irradiation on colour stability and inhibiting effects of copigmentation against degradation influence of UV irradiation on anthocyanin were evaluated. Copigmentation of anthocyanin resulting in increase in both hyperchromic effects and bathochromic shifts. In this study tannic acid has the biggest hyperchromic effect and benzoic acid has the lowest hyperchromic effect. Four levels of copigment concentration as: 1:0, 1:20, 1:40, 1:60 were examined. The copigmentation effect increased with copigment containe. UV irradiation has the biggest effect on the copigmentation complex in comparison with heating. The suitable pH for copigmentation complex was in pH 3.5. In this study, tannic acid predominate copigment among copigments.
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How to cite this article
Mohammad Abyari, Reza Heidari and Rashid Jamei, 2006. The Effects of Heating, UV Irradiation and pH on Stability of Siahe Sardasht Grape Anthocyanin-copigment Complex. Journal of Biological Sciences, 6: 638-645.
DOI: 10.3923/jbs.2006.638.645
URL: https://scialert.net/abstract/?doi=jbs.2006.638.645
DOI: 10.3923/jbs.2006.638.645
URL: https://scialert.net/abstract/?doi=jbs.2006.638.645
REFERENCES
- Bakowska, A., A.Z. Kucharska and J. Oszmianski, 2003. The effects of heating, UV irradiation and storage on stability of the anthocyanin-polyphenol copigment complex. Food Chem., 81: 349-355.
CrossRefDirect Link - Baranac, J.M., N.A. Petranovic and J.M. Dimitric-Marcovic, 1997. Spectrophotometric study of anthocyanin copigmentation reactions.2. Malvin and the nonglycosidized flavone morin. J. Agric. Food Chem., 45: 1698-1700.
Direct Link - Brouillard, R. and O. Dangles, 1994. Anthocyanin molecular interactions: The first step in the formation of new pigments during wine aging. Food Chem., 51: 365-371.
Direct Link - Brouillard, R., M.C. Wig, O. Dangles and A. Cheminat, 1991. The pH and solvent effects on the copigmentation reaction of malvin with polyphenols, purine and pyrimidin derivatives. J. Chem. Soc. Perkin Trans., 2: 1235-1241.
CrossRef - Brouillard, R., G. Mazza, Z. Saad, A.M. Albecht-Gary and A. Cheminat, 1989. The copigmentation reactions of anthocyanins: A microprobe for the structural study of aqueous solution. J. Am. Chem. Soc., 111: 2604-2610.
CrossRef - Cai, Y., T.H. Lilley and E. Haslam, 1990. Polyphenol-anthocyanin copigmentation. J. Chem. Soc. Chewm. Commun., 4: 380-383.
CrossRef - Dao, L.T., G.R. Tkeoka, R.H. Edwards, J. Berrios and J. De, 1998. Improved method for stabilization of anthocyanins. J. Agric. Food Chem., 46: 3564-3569.
CrossRef - Daris, J. Martin, B. Martin-Luis, M. Carrillo-Lopez, R. Lamuela Raventos, C. Diaz-Romero and R. Boulton, 2002. Effect of caffeic acid on the color of red wine. J. Agric. Food Chem., 27: 2062-2067.
CrossRef - Martin, D.J., C. Marta, D. Eugenio and B.B. Roger, 2001. Enhancement of red wine colour by pre-fermentation addition of copigments. Food Chem., 73: 217-220.
Direct Link - Dangles, O. and R. Brouillard, 1992. A spectroscopic method based on the anthocyanin copigmentation interaction and applied to the quantative study of molecular complexes. J. Chem. Soc. Perkin Trans., 2: 247-257.
Direct Link - Davis, A.J. and G. Mazza, 1993. Copigmentation of simple and acylated anthocyanins with colorless phenolic compounds. J. Agric. Food Chem., 41: 716-720.
Direct Link - Eiro, M.J. and M. Heinonen, 2002. Anthocyanin color behavior and stability during storage: Effect of intermolecular copigmentation. J. Agric. Food Chem., 4: 7461-7466.
CrossRefDirect Link - Gao, L. and G. Mazza, 1994. Quantitation and distribution of simple and acylated anthocyanins and other phenolics in blueberries. J. Food Sci., 59: 1057-1059.
CrossRef - Marcovic, D.J.M., N.A. Petranovic and J.M. Baranac, 2005. The copigmentation effect of sinapic acid on malvin: A spectroscopic ionvestigation on colour enhancement. J. Photochem. Photobiol. B: Biol., 78: 223-228.
Direct Link - Mazza, G. and R. Brouillard, 1987. Recent developments in the stabilization of anthocyanins in food products. Food Chem., 25: 207-225.
CrossRefDirect Link - Osmianski, J., A. Bakowska and S. Piacente, 2004. Thermodynamic characteristics of copigmentation reaction of acylated anthocyanin isolated from blue flowers of Scutellaria baicalensis Georgi with copigments. J. Sci. Food Agric., 84: 1500-1506.
CrossRefDirect Link - Wang, H., G. Cao and R.L. Prior, 1997. Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem., 45: 304-309.
CrossRefDirect Link - Yoshida, K., R. Okuno, K. Kameda, M. Mori and T. Kondo, 2003. Influence of E, Z-isomerization and stability of acylated anthocyanins under the UV irradiation. Biochem. Eng. J., 14: 163-169.
Direct Link