Ghazala Nasim
Department of Botany, University of the Punjab, Ouaid-1-Azam Campus, Lahore-54590, Pakistan
Shahid Hameed Malik
Department of Botany, University of the Punjab, Ouaid-1-Azam Campus, Lahore-54590, Pakistan
Rukhsana Bajwa
Department of Botany, University of the Punjab, Ouaid-1-Azam Campus, Lahore-54590, Pakistan
M. Afzal
Department of Botany, University of the Punjab, Ouaid-1-Azam Campus, Lahore-54590, Pakistan
Salman Wajid Mian
National Institute for Biotechnology and Genetic Engineering (NIBGE),
P.O. Box 577, Faisalabad, Pakistan
ABSTRACT
The sporocarps obtained from NARC, Islamabad and local market were utilized as a source of tissue culturing. Initial culturing was done on malt extract agar plates. The discs (0.5 cm in diameter) from actively growing culture plates of three different mushrooms were planted on the fresh media plates of Malt extract agar medium (MEA), Murashige and Skoogs (MS) medium and potato dextrose agar (PDA) medium. Three replicates were taken in each case. An increase in diameter of the culture discs was recorded daily. The results indicted that the mycelial growth of Pleorotus ostreatus, varieties sajar, caju , citydeosus and Volvariella volvacea were maximum in medium plates containing MEA medium. The results were significant at P=0.05 level of significance. While potato dextrose agar medium (PDA), ended up with slowest growth.
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How to cite this article
Ghazala Nasim, Shahid Hameed Malik, Rukhsana Bajwa, M. Afzal and Salman Wajid Mian, 2001. Effect of Three Different Culture Media on Mycelial Growth of Oyster and Chinese Mushrooms. Journal of Biological Sciences, 1: 1130-1133.
DOI: 10.3923/jbs.2001.1130.1133
URL: https://scialert.net/abstract/?doi=jbs.2001.1130.1133
DOI: 10.3923/jbs.2001.1130.1133
URL: https://scialert.net/abstract/?doi=jbs.2001.1130.1133
Samuel P Monteon Reply
Excellent write-up. I was wondering this exact question when choosing my own media of preference. Thank you for sharing.