Basavaraj Binjawadagi
Department of Veterinary Microbiology, Veterinary College, KVAFSU, Bidar, Karnataka, India
Y. Hari Babu
Department of Veterinary Microbiology, Veterinary College, KVAFSU, Bidar, Karnataka, India
E. Sreekumar
Department of Veterinary Microbiology, Veterinary College, KVAFSU, Bidar, Karnataka, India
ABSTRACT
Present study was undertaken to evaluate the immunomodulatory effect of recombinant chicken interferon -gamma (rchIFN-γ) on specific and non-specific immune responses in chicken vaccinated against Newcastle Disease Virus (NDV). A total of 180 day old layer chicks (BV-300) were divided into three groups. Test Group I (TG-I) (64 chicks) was injected with 5 microgram/chick subcutaneously of rchIFN-γ along with ND vaccinations (LaSota and R2B strains), Test Group II (TG-II) (64 chicks) was injected with same dose and route of rchIFN-γ 6 h after ND vaccinations on both occasions, whereas, third group remained as vaccinated control group (52 chicks). Haemagglutination Inhibition (HI) test and Migration Inhibition (MI) in percentages estimated by Leukocyte Migration Inhibition Test (LMIT) were conducted to evaluate humoral and cell mediated immune responses respectively. Nitroblue Tetrazolium (NBT) reduction assay was conducted to evaluate the non-specific immune response by estimating the Phagocytic Index (PI) in percentages. The test results revealed that, significantly (p< 0.05) increased specific (humoral and cell mediated) and non-specific immune responses were recorded in the groups treated with rchIFN-γ i.e., higher results when given along with the vaccine and highest results when given 6 h after vaccinations. The results advocated that, rchIFN-γ may be used as an immunopotentiator in chicken vaccinated against NDV.
PDF References Citation
How to cite this article
Basavaraj Binjawadagi, Y. Hari Babu and E. Sreekumar, 2009. Immunomodulatory Effect of Recombinant Chicken Interferon-gamma (rchIFN-γ) on Specific and Non-specific Immune Responses in Chicken Vaccinated Against Newcastle Disease Virus (NDV). International Journal of Poultry Science, 8: 122-127.
DOI: 10.3923/ijps.2009.122.127
URL: https://scialert.net/abstract/?doi=ijps.2009.122.127
DOI: 10.3923/ijps.2009.122.127
URL: https://scialert.net/abstract/?doi=ijps.2009.122.127
REFERENCES
- Anderson, K.P., E.H. Fennie and T. Yilma, 1988. Enhancement of secondary antibody response to vesicular stomatitis virus G protein by interferon-gamma treatment at primary immunization. J. Immunol., 140: 3599-3604.
Direct Link - Campos, M., H.B. Ohamann, D. Hutchings, N. Rapin, L.A. Babiuk and M.J. Lawman, 1989. Role of interferon-gamma in inducing cytotoxicity of peripheral blood mononuclear leukocytes to bovine herpes virus type-1 (BHV-1) infected cells. Cell. Immunol., 120: 259-269.
PubMed - Chiang, Y.W., H. Murata and J.A. Roth, 1991. Activation of bovine neutrophils by bovine tumor necrosis factor alpha. Vet. Immunol. Immunopathol., 29: 329-338.
PubMed - Erhard, M.H., P. Schimidt, P. Zismeister, A. Hofmann and U. Munster et al., 2000. Adjuvant effects of various lipopeptides and interferon gamma on humoral immune response of chickens. Poult. Sci., 79: 1264-1270.
Direct Link - Farnell, M.B., M. El-Halawani, S. You, A.P. McEIrov, B.M. Hargis and D.J. Coldwel, 2001. In vivo biologic effects of recombinant turkey interferon gamma in neonatal leghorn chicks: Protection against Salmonella enteriditis organ invasion. Avian Dis., 45: 473-478.
PubMedDirect Link - Frasca, D., L. Adorini, S. Landolfo and G. Doria, 1985. Enhancing effect of Interferon-gamma on helper T-cell activity and IL-2 production. J. Immunol., 134: 3907-3911.
Direct Link - Yeh, H.Y., B.J. Winslow, D.E. Junker and J.M. Sharma, 1999. In vitro effects of recombinant interferon gamma on immune cells. J. Interferon Cytokine Res., 19: 687-691.
PubMed - Kogut, M.H., L. Rothwell and P. Kaiser, 2005. Interferon gamma priming of chicken heterophils upregulates the expression of proinflammatory and Th1 cytokine mRNA following receptor mediated phagocytosis of salmonella enteriditis. J. Interferon Cytokine Res., 25: 73-81.
PubMed - Lowenthal, J.W., J.J. York, T.E. O'Neil, S. Rhodes, S.J. Prowse, A.D.G. Strom and M.R. Digby, 1997. In vivo effects of chicken interferon-γ during infection with Eimeria. J. Interferon Cytokine Res., 17: 551-558.
CrossRefDirect Link - Mifune, K., K. Mannen, S. Cho and H. Narahara, 1987. Enhanced antibody response in mice by combined administration of interferon with rabies vaccine. Arch. Virol., 94: 287-295.
CrossRef - Murrey, H.W., 1988. Interferon gamma, the activated macrophage and host defense against microbial challenge. Ann. Inter. Med., 108: 595-608.
Direct Link - Nagahata, H., A. Yatra and H. Noda, 1986. The evaluation of a quantitative assay for estimating the bactericidal activity of bovine neutrophils by nitro blue tetrazolium reduction assay. Br. Vet. J., 142: 578-584.
PubMed - Naylor, P.F. and T.W.A. Little, 1975. A simple method for isolating leucocytes from bovine blood and their separation into lymphocytes and granulocytes. Res. Vet. Sci., 18: 336-338.
Direct Link - Park., B.H., S.M. Fikrig and E.M. Smithwick, 1968. Infection and nitroblue tetrazolium reduction by neutrophils. Lancet, 7: 532-534.
Direct Link - Roth, J.A. and D.E. Frank, 1989. Recombinant bovine interferon gamma as an immunomodulator in dexamethasone treated and non-treated cattle. J. Interferon Res., 9: 143-151.
Direct Link - Semnani, M.J., M.B. Kabbur and N.C. Jain, 1993. Activation of bovine neutrophil functions by IFN-gamma, tumor necrosis factor-alpha and interleukin-1alpha. Comp. Haematol. Int., 3: 81-88.
CrossRefDirect Link