Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References

Research Article
Monitoring of Pesticide Residues in Saudi Arabia Agricultural Soils

M.I. Al- Wabel, M.H. El- Saeid, A.M. Al- Turki and G. Abdel- Nasser
 
ABSTRACT
The objective of our study was to monitor the multiple pesticide residues in soil samples collected from 15 regions around the Saudi Arabia. Fifteen regions were selected at Central (Dereiah, Deirab, Kharj, Mezahmiah and Qaseem); East (Qatif, Al-Hasa and Haradh); West (Taif); North (Tabouk, Jouf and Hail) and South (Abha, Gizan, Wadi Al-Dwaser) of Saudi Arabia to perform the survey process of pesticide residues. Soil samples were collected from nine sites for each region from surface layer (0-30 cm depth) and sub-surface layer (30-60 cm depth). A simple Microwave-Assisted Extraction (MAE) technique and gas chromatography with mass spectrometer detector were modified and applied as extraction and analytical techniques, respectively. The major groups of pesticides tested were Organochlorines (OCPs), Organophosphorus (OPPs), Carbamates, Pyrethroids (PYR) and Herbicides in different soil samples. The overall data showed that the highest concentration of pesticide residues was differed according to the pesticide type, soil layer and regions. Twelve pesticide residues were detected in soil samples collected from different regions and ranged as follows; Dimethoate (0.007-0.700 mg kg-1); Chloroneb (0.011-0.4 mg kg-1); Methomyl (0.018-0.405 mg kg-1); Oxamyl (0.014-0.150 mg kg-1); Toxaphen (0.006-0.162 mg kg-1); pp-DDT (0.080-0.575 mg kg-1); pp-DDE (0.1-0.525 mg kg-1); Monocrotophos (0.021-0.047 mg kg-1); Chlorpyrifos (0.12-0.24 mg kg-1); Diazinon (0.024 mg kg-1); Cypermethrin (0.012-0.205 mg kg-1) and Lindane (0.290-394 mg kg-1). The results indicated that Dimethoate, Chloroneb, Methomyl, Oxamyl and Toxaphen were the more contamination sources in the studied regions. Also, Gizan region was more contaminated with pesticide residues followed by Wadi Al-Dwaser and Abha regions.
Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

M.I. Al- Wabel, M.H. El- Saeid, A.M. Al- Turki and G. Abdel- Nasser, 2011. Monitoring of Pesticide Residues in Saudi Arabia Agricultural Soils. Research Journal of Environmental Sciences, 5: 269-278.

DOI: 10.3923/rjes.2011.269.278

URL: http://scialert.net/abstract/?doi=rjes.2011.269.278
 
Received: June 16, 2010; Accepted: July 31, 2010; Published: August 23, 2010

INTRODUCTION

Historically, as an agricultural terms, a persistent pesticide was considered to be remaining in the soil in significant quantities after application until the next growing seasons (Sun and Lee, 2003; Gong et al., 2004; Craven and Hoy, 2005). Pesticides have been used in agriculture for decades and they are distributed widely across the globe, even in pristine regions. This results in wide concentration levels of their residues in the soils and the environment. They are among the hazardous contaminants and can persist in soils for decades even after being banned (Goncalves et al., 2006; Hussen et al., 2007; Oldal et al., 2006; Shegunova et al., 2007). The common use of pesticides can cause pollution for the environment and became a potential and/or deliberate risk to human health due to their potential carcinogenic effects (El-Saeid, 1999; Brock et al., 2000).

Organochlorine Pesticides (OCPs) have been a major environmental issue, attracting both scientific and public concern because of their nature of toxicity, persistence, health problems and endocrine disrupting effects (Wang et al., 2008). It has also a great concern due to their high bioaccumulation potential, ubiquity, persistence in the environment, deleterious effect and high toxicity to non-target organisms (Jones and de Voogt, 1999; Hao et al., 2008). Recent investigations showed that the persistence of DDTs and HCHs has left considerable amount of residues in soils for many areas (Zhang et al., 2006; Wang et al., 2007; Cai et al., 2008). The OCPs residues in soils showed a temporal and spatial changes in the world (Skrbic and Durisic-Mladenovic, 2007) because the residue level of OCPs depends on the balance of inputs and disappearance (such as decomposition, leaching and volatilization). Their concentration in the environment are affected by many factors including application history and agricultural practices (Wang et al., 2006) and nature and persistence of the pesticides. For several years, organophosphorus pesticides (OPPs) and pyrethroid pesticides (PYR) are widely used in agriculture, especially in the developing countries. Although, the use of OPP and PYR pesticides increase crop production, their usage have a great deal to the environment (Wang et al., 2008).

The Microwave Asisted Extraction (MAE) techniques were used by Ganzler et al. (1986), to extract anti-nutritive compounds from various plant materials. Since then, microwave methodologies have been adapted for other scientific applications, including the extraction of pesticides (Prados-Rosales et al., 2002; Fuentes et al., 2006; Sporring et al., 2005; Tavares et al., 2005). There are many advantages of MAE procedures such as reduced solvent usage and shorter analysis time. There are the possibilities of new applications of MAE and several publications have describing the extraction methodologies (Barriada-Pereira et al., 2003; Sun and Lee, 2003; Fuentes et al., 2006; Yuan et al., 2006; CEM Corporation, 2000).

The aims of the present study are to demonstrate the practical applicability of MAE in combination to gas chromatography-mass spectrometry (GC-MS) for the surveying and determining of pesticide residues in different soil samples collected from Saudi Arabia.

MATERIALS AND METHODS

Chemicals and reagents: Pesticide standards (Dimethoate, Chloroneb, Methomyl, Oxamyl, Toxaphen, DDT, DDE, Monocrotophos, Chlorpyrifos, Diazinon, Cypermethrin and Lindane,) were provided in the year of 2007 by Chemservice, USA. All pesticide standards were of 98-99% purity. Hexane and Acetone used for residues analysis were analytical reagent grade. The pesticides stock and working solutions were prepared in acetone.

Soil sampling collection: Total of fifteen regions (Table 1) were selected at Central (Dereiah, Deirab, Kharj, Mezahmiah and Qaseem); East (Qatif, Al-Hasa and Haradh); West (Taif); North (Tabouk, Jouf and Hail ) and South (Abha, Gizan, Wadi Al-Dwaser ) of Saudi Arabia to explore pesticide residues in soils (Fig. 1). Soil samples were collected from three locations for each region. For each location, three sites were selected to collect the soil samples from surface layer (0-30 cm depth) and sub-surface layer (30-60 cm depth). The soil samples were placed in clean plastic sheet and mixed up carefully on the site with a small shovel. Approximately, one kg portion of each sample was collected. The soil samples were air dried and sieved through a 2.0 mm sieve and then were prepared to extract the pesticides residues (Al-Turki et al., 2009).

Table 1: List of regions for soil sampling sites in Saudi Arabia

Fig. 1: Soil sampling sites in Saudi Arabia

Preparation of spiked soil samples: Spiked soil samples were prepared by adding an appropriate volume of spiking solution (5 mg L-1 of pesticides in acetone) to five grams of soil. The spiked samples were prepared just before analysis, waiting approximately 30 min until solvent evaporation. Soil sample was extracted with both Microwave-Assisted Extraction, MAE (Ganzler et al., 1986) and Soxhlet extraction techniques (Singh et al., 2007; Carvalho et al., 2008; Schreck et al., 2008). The main purpose of this step is to determine the average recovery percent of investigated pesticides by both extraction techniques.

Minimum detection limit (MDL): To determine the MDL and perform the GC-MS quantification using a four-point calibration curve plotting peak area versus mg L-1 concentration of 12 pesticides using the dilution levels ranged from 0.0001 to 5.00 mg L-1.

Extraction of pesticide residue by MAE: The proposed EPA Method 3540C (USEPA, 1996) and CEM application note No. E003 (CEM Corporation, 2000) was modified and used during conducting of the present study. A microwave assisted extraction (MAE) system MES-1000, CEM Corporation, Matthews, NC, USA with Lined Extraction Vessels (LEV).

In the preliminary studies, the effects of temperature, microwave power, extraction time and solvent volume were tested (Table 1), to extract different groups of pesticide residues from soil samples. For this purpose, 5.0 g of soil was weighed in a tetrafluoromethaxil (TFM) microwave extraction vessel with addition of 1 mL of an aqueous spiking solution containing mixture of the pesticide standard solution. Soil samples were equilibrated by shaking for 1 h before microwave extraction. Finally, all samples extracted under different conditions as will show in the results to obtain the optimal MAE conditions with this procedure. Finally the optimum extraction conditions with acceptable recovery rate was mixture of 5.0 g soil samples with extraction solvent of (Acetone: Hexane, 3:2 on v/v base), 80 psi pressure, 60% microwave power, 120°C temperature and 20 min extraction time. After extraction, soil extracts were filtered and evaporated to dryness; then the residue was re-dissolved and directly analyzed by gas chromatography mass spectrometer (GC-MS). By this procedure, clean chromatograms were obtained without any additional cleanup step.

Gas Chromatography-Mass Spectrometer (GC-MS) analysis: The extracts of pesticide residues in soil samples extracted by MAE technique were performed and analyzed by a HP 5890 series II plus GC coupled to an HP 5972 Mass Selective Detector. The GC columns were a DB-5 fused silica capillary column (30 mx0.32 mm ID, 1 μm film thickness; J and W Scientific, Folsom, CA). One microliter of the extract was injected split-less injector at 250°C temperature, on the GC-MS for analysis. The temperature program for the GC was as follows: isothermal for 1 min at 100°C, increased at a rate of 10°C min-1 up to 240°C and isothermal for 15 min. Helium was used as a carrier gas (1.2 mL min-1). Quantification was performed using a four-point calibration curve plotted peak area versus mg L-1 concentration. The results were expressed in percentage recovery of the pesticides.

Quality control: Recoveries were determined for all samples by spiking with the mix of pesticide standards prior to extraction. Recoveries were higher than 98% for all samples and the recovery factors were not applied to any of the data. Analytical method recoveries for the given set of pesticides were also determined by repeating the whole analytical procedure with the reference soil enriched by the standard mixture of the pesticides of the known concentration.

Table 2: Pesticide recovery ± RSD, (%) of spiked soil samples extracted by MAE and determined with GC-MS

They ranged from 98 to 102.44% for all tested compounds as showed in (Table 2). Reproducibility was calculated based on the replicate analyses, with relative standard deviation (RSD,%) less than 2.48%. All laboratory blanks were below the detection limits.

RESULTS AND DISCUSSION

Average pesticide residue concentrations in different regions of Saudi Arabia agricultural soils from surface (0-30 cm) and subsurface (30-60 cm) layers are summarized in Table 3 and 4. The collected soil samples represent five areas as shown in Table 1. The highest concentration of pesticide residues was differed according the pesticide type. The highest concentrations in surface layer were 0.7 mg kg-1 for Dimethoate at Abha. In subsurface layer, the highest concentration was 0.575 mg kg-1 for pp-DDT at Wadi Al-Dwaser (Table 4).

The present results indicated that soil surface layer as general has higher concentrations than soil subsurface layer (Ahmed et al., 1998). Also Gizan region was more contaminated with pesticide residues (5 pesticide residues) followed by Wadi Al-Dwaser and Abha regions (3 pesticide residues for each). Other regions have one or two pesticide residues only.

Twelve pesticide residues were detected in soil samples collected from different regions as follows; Dimethoate (15 region); Chloroneb (12 regions); Methomyl (11 regions); Oxamyl and Toxaphen (8 regions); pp-DDT and pp-DDE (4 regions); Cypermethrin (3 regions); Monocrotophos (2 regions); Chlorpyrifos, Diazinon and Lindane (one region). Therefore, the results indicated that Dimethoate, Chloroneb, Methomyl, Oxamyl and Toxaphen were the more contamination sources in the studied regions.

The most important notice in the results of soil analysis was the detection of the residue of pp-DDT and one of their derivatives pp-DDE. Both pesticide residues were detected in 4 regions. The concentration of pp-DDT was found to be higher than that of pp-DDE in soil samples from all regions, because DDE is one of the primary metabolites of DDT. All detected pesticide residues in our study were higher than pesticide residues detected in other studies. These results may be due to the extensive use of organochlorine insecticides and their long resistance in the investigated regions (Harner et al., 1999; Ribes and Grimalt, 2002; Zhang et al., 2005, 2006; Wang et al., 2007, 2008; Shegunova et al., 2007; Li et al., 2008).

Table 3: Average pesticide residue concentrations (mg kg-1) in soil samples collected from surface layer (0-30 cm) of different Saudi Arabia regions
*ND not detected

Table 4: Average pesticide residue concentrations (mg kg-1) in soil samples collected from subsurface layer (30-60 cm) of different Saudi Arabia regions
*ND not detected

The highest concentration of pesticide residues in surface layer was found in southern part of Saudi Arabia. The concentrations were 0.443 mg kg-1 for Dimethoate; 0.249 mg kg-1 for Chloroneb; 0.405 mg kg-1 for Methomyl; 0.150 mg kg-1 for Oxamyl; 0.238 mg kg-1 for pp-DDT; 0.175 mg kg-1 for pp-DDe and 0.240 mg kg-1 for Chlorpyrifose. But the highest value of Cypermethrin (0.205 mg kg-1) was at Western region and Lindane (0.394 mg kg-1) at Central region. For subsurface layer, the highest values were found in southern region (0.302 mg kg-1 for Dimethoate; 0.158 mg kg-1 for Chloroneb; 0.245 mg kg-1 for Methomyl; 0.075 mg kg-1 for Oxamyl; 0.377 mg kg-1 for pp-DDT; 0.328 mg kg-1 for pp-DDE and 0.120 mg kg-1 for Chlorpyrifos). The highest values of Monocrotophos (0.047 mg kg-1); Diazinon (0.024 mg kg-1); Lindane (0.290 mg kg-1) were detected in central region. Toxaphen (0.121 mg kg-1) was detected in highest value in eastern region.

The general distribution of studied pesticides reflects the agricultural use of these pesticides in all region overall Saudi Arabia. Some of these pesticides were never used in these regions, other were used in small or large quantities, such as in southern region.

Organochlorine Pesticides (OCPs) attracted wide concern for decades because of their nature of persistence, biomagnification and high toxicity to non-target organisms (Nakata et al., 2002). Some of OCPs are even identified as endocrine disrupting pesticides by USEPA (1998). OCPs were well documented in urban and suburban soils (Ma et al., 2003; Zhu et al., 2005, Zhang et al., 2005; Li et al., 2006, 2008). The chemical properties of organochlorine pesticides such as low water and high fat solubility, stability to photo-oxidation and low vapour pressure are the main factors not only in the efficacy of these compounds as pesticides but also in their persistence in the environment.

According to the environmental quality standard for soils (Wang et al., 2008), the quality of soil was classified as having little pollution (below 0.05 mg kg-1), slight pollution (between 0.05-0.5 mg kg-1), moderate pollution (between 0.5-1.0 mg kg-1) and heavy pollution (>1.0 mg kg-1). When compared the levels of present study could be defined as having slight pollution in most regions and moderate pollution in few regions (Table 3, 4).

CONCLUSION

Most of the compounds studied were recovered in good yields with Relative Standard Deviations (RSDs) below 2.48%. The averages of MDL ranged from 0.0001 to 0.004 mg L-1 for MAE. The MAE modified method was successfully applied to the extraction of 12 pesticide residues namely, Dimethoate, Chloroneb, Methomyl, Oxamyl, Toxaphen, DDT, DDE, Monocrotophos, Chlorpyrifos, Diazinon, Cypermethrin and Lindane from soils which was considered an appropriate index to establishing its applications. Further characterization of pesticide residues in soil and their mobility and transport in soil profile would be useful. Also, the interaction between pesticide residues and soil properties will be good point to study. The present results indicated that soil surface layer as general has higher concentrations than soil subsurface layer. Also, Gizan region was more contaminated with pesticide residues followed by Wadi Al-Dawaser and Abha regions. MAE and GC-MS techniques were accurate, reliable and less time consuming in the analysis of different surface and sub-surface layer soil samples and are recommended for the monitoring of pesticides with a broad range of physico-chemical properties in soils.

ACKNOWLEDGMENT

The authors thank the Deanship of Scientific Research at King Saud University for financial support through Project# DSR-AR-2-(14)

REFERENCES
Ahmed, M., S. Ismail and S. Mabrouk, 1998. Residues of some chlorinated hydrocarbon pesticides in rainwater, soil and ground water and their influence on some soil microorganisms. Environ. Int., 24: 665-670.
Direct Link  |  

Al-Turki, A.M., G. Abdel-Nasser, M.I. Al-Wabel and M.H. El-Saeid, 2009. Evaluation of pollutants in agricultural soils in Saudi Arabia. Financial Supported from Deanship of Scientific Research, King Saud University. Two Parts, pp: 649.

Barriada-Pereira, M., E. Concha-Grana, M.J. Gonzalez-Castro, S. Muniategui-Lorenzo, P. Lopez-Mahia, D. Prada-Rodrıguez and E. Fernandez-Fernandez, 2003. Microwave-assisted extraction versus Soxhlet extraction in the analysis of 21 organochlorine pesticides in plants. J. Chromatogr. A., 1008: 115-122.
CrossRef  |  

Brock, T.C.M., R.P.A. van Wijngaarden and G.J. van Geest, 2000. Ecological Risk of Pesticides in Freshwater Ecosystems. Part 2: Insecticides. Green World Research, Wageningen, The Netherlands.

CEM Corporation, 2000. PAHs from sludge HPLC method. Application note # E003. CEM Corporation, Friendswood, TX 77549.

Cai, Q.Y., C.H. Mo, Q.T. Wu, A. Katsoyiannis and Q.Y. Zeng, 2008. The status of soil contamination by semivolatile organic chemicals (SVOCs) in China: A review. Sci. Total Environ., 389: 209-224.
PubMed  |  

Carvalho, P.N., P.N.R. Rodrigues, F. Alves, R. Evangelista, M.C.P. Basto and M.T.S.D. Vasconcelos, 2008. An expeditious method for the determination of organochlorine pesticides residues in estuarine sediments using microwave assisted pre-extraction and automated headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry. Talanta, 76: 1124-1129.
CrossRef  |  

Craven, A. and S. Hoy, 2005. Pesticide persistence and bound residues in soil-regulatory significance. Environ. Pollut., 133: 5-9.
CrossRef  |  

El-Saeid, M.H., 1999. New techniques for residue analysis of pesticides in foods. Ph.D. Thesis, Texas Southern University and Al-Azhar University, Faculty of Agriculture, Cairo, Egypt.

Fuentes, E., M.E. Baez and D. Rey, 2006. Microwave-assisted extraction through an aqueous medium and simultaneous cleanup by partition on hexane for determining pesticides in agricultural soils by gas chromatography: A critical study. Anal. Chim. Acta, 578: 122-130.
CrossRef  |  

Ganzler, K., A. Salgo and K. Valko, 1986. Microwave extraction: A novel sample preparation method for chromatography. J. Chromatogr. A., 371: 299-306.
CrossRef  |  

Goncalves, C., J.J. Carvalho, M.A. Azenha and M.F. Alpendurada, 2006. Optimization of supercritical fluid extraction of pesticide residues in soil by means of central composite design and analysis by gas chromatography–tandem mass spectrometry. J. Chromatography A, 1110: 6-14.
CrossRef  |  

Gong, Z.M., S. Tao, F.L. Xu, R. Dawson and W.X. Liu et al., 2004. Level and distribution of DDT in surface soils from Tianjin, China. Chemosphere, 54: 1247-1253.
CrossRef  |  

Hao, H., B. Sun and Z. Zhao, 2008. Effect of land use change from pady to vegetable field on the residues of organochlorine pesticides in soils. Environ. Pollut., 156: 1046-1052.
PubMed  |  

Harner, T., J.L. Wideman, L.M.M. Jantunen, T.F. Bidleman and W.J. Parkhurst, 1999. Residues of organochlorine pesticides in Alabama soils. Environ. Pollution, 106: 323-332.
CrossRef  |  

Hussen, A., R. Westbom, N. Megersa, L. Mathiasson and E. Bjorklund, 2007. Selective pressurized liquid extraction for multi-residue analysis of organochlorine pesticides in soil. J. Chromatography A, 1152: 247-253.
CrossRef  |  

Jones, K.C. and P. de Voogt, 1999. Persistent Organic Pollutants (POP): State of the science. Environ. Pollut., 100: 209-221.
CrossRef  |  

Li, X. , H. Zhu, X. Liu, H. Fu, X. Xu and H. Cheng, 2006. Distribution of HCHs and DDTs in soils from Beijing City, China. Arch. Environ. Contam. Toxicol., 51: 329-336.
CrossRef  |  

Li, X.H., W. Wang, J. Wang, X.l. Cao and X.F. Wang et al., 2008. Contamination of soils with organochlorine pesticides in urban parks in Beijing, China. Chemosphere, 70: 1660-1668.
CrossRef  |  

Ma, L.L., S.G. Chu and X.B. Xu, 2003. Organic contamination in the greenhouse soils from Beijing suburbs, China. J. Environ. Monit., 5: 786-790.
CrossRef  |  

Nakata, H., M. Kawazoe, K. Arizono, S. Abe and T. Kitano et al., 2002. Organochlorine pesticides and polychlorinated biphenyl residues in foodstuffs and human tissues from china: Status of contamination, historical trend and human dietary exposure. Arch. Environ. Contam. Toxicol., 43: 473-480.
CrossRef  |  

Oldal, B., E. Maloschik, N. Uzinger, A. Anton and A. Szekacs, 2006. Pesticide residues in Hungarian soils. Geoderma, 135: 163-178.
CrossRef  |  

Prados-Rosales, R.C., M.C. Herrera, J.L. Luque-Garcıa and M.D. Luque de Castro, 2002. Study of the feasibility of focused microwave-assisted Soxhlet extraction of N-methylcarbamates from soil. J. Chromatogr. A, 953: 133-140.
CrossRef  |  

Ribes, A. and J.O. Grimalt, 2002. Temperature and organic matter dependence of the distribution of organochlorine compounds in mountain soils from the subtropical Atlantic (Teide, Tenerife island). Environ. Sci. Technol., 36: 1879-1885.

Schreck, E., F. Gereta, L. Gontier and M. Treilhou, 2008. Development and validation of a rapid multiresidue method for pesticide determination using gas chromatography-mass spectrometry: A realistic case in vineyard soils. Talanta, 77: 298-303.
CrossRef  |  

Shegunova, P., J. Klanova and I. Holoubek, 2007. Residues of organochlorinated pesticides in soils from the Czech Republic. Environ. Pollut., 146: 257-261.
CrossRef  |  

Singh, S.B., G.D. Foster and S.U. Khan, 2007. Determination of thiophanate methyl and carbendazim residues in vegetable samples using microwave-assisted extraction. J. Chromatogr. A, 1148: 152-157.
CrossRef  |  

Skrbic, B. and N. Durisic-Mladenovic, 2007. Principal component analysis for soil contamination with organochlorine compounds. Chemosphere, 68: 2144-2152.
CrossRef  |  

Sporring, S., S. Bøwadt, B. Svensmark and E. Bjorklund, 2005. Comprehensive comparison of classic Soxhlet extraction with Soxtec extraction, ultrasonication extraction, supercritical fluid extraction, microwave assisted extraction and accelerated solvent extraction for the determination of polychlorinated biphenyls in soil. J. Chromatogr. A, 1090: 1-9.
CrossRef  |  

Sun, L. and H.K. Lee, 2003. Optimization of microwave-assisted extraction and supercritical fluid extraction of carbamate pesticides in soil by experimental design methodology. J. Chromatogr. A, 1014: 165-177.
CrossRef  |  

Tavares, O., S. Morais, P. Palga and C. Delerue-Matos, 2005. Determination of ametryn in soils via microwave-assisted solvent extraction coupled to anodic stripping voltammetry with a gold ultramicroelectrode. Anal. Bioanal. Chem., 382: 477-484.
CrossRef  |  

USEPA, 1996. Soxhlet extraction. Method 3540C. http://www.epa.gov/waste/hazard/testmethods/sw846/pdfs/3540c.pdf.

USEPA, 1998. Endocrine disruptor screening and testing advisor committee. Final Report. http://www.epa.gov/endo/pubs/edspoverview/edstac.htm.

Wang, F., X. Jiang, Y.R. Bian, F.X. Yao and H.J. Gao et al., 2007. Organochlorine pesticides in soils under different land usage in the Taihu Lake region, China. J. Environ. Sci., 19: 584-590.

Wang, F., Y.R. Bian, X. Jiang, H.J. Gao, G.F. Yu and J.C. Deng, 2006. Residual characteristics of organochlorine pesticides in Lou soils with different fertilization modes. Pedosphere, 16: 161-168.
CrossRef  |  

Wang, X., D. Wang, X. Qin and X. Xu, 2008. Residues of organochlorine pesticides in surface soils from college school yards in Beijing, China. J. Environ. Sci., 20: 1090-1096.
CrossRef  |  

Yuan, S., M. Tian and X. Lu, 2006. Microwave remediation of soil contaminated with hexachlorobenzene. J. Hazardous Mater., 137: 878-885.
CrossRef  |  

Zhang H.B., Y. Lu, R.W. Dawson, Y. Shi and T. Wang, 2005. Classification and ordination of DDT and HCH in soil samples from the guanting reservoir, China. Chemosphere, 60: 762-769.
CrossRef  |  

Zhang, H.B., Y.M. Luo, Q.G. Zhao, M.H. Wong and G.L. Zhang, 2006. Residues of organochlorine pesticides in Hong Kong soils. Chemosphere, 63: 633-641.
CrossRef  |  

Zhu, Y., H. Liu, Z. Xi, H. Cheng and X. Xu, 2005. Organochlorine pesticides (DDTs and HCHs) in soils from the outskirts of Beijing, China. Chemosphere, 60: 770-778.
CrossRef  |  

©  2014 Science Alert. All Rights Reserved
Fulltext PDF References Abstract