Mei Musa Ali
Central Laboratory, Ministry of Science and Technology, Khartoum, Sudan
Mawahib Eisa
Central Laboratory, Ministry of Science and Technology, Khartoum, Sudan
Mohammed Idrees Taha
Central Laboratory, Ministry of Science and Technology, Khartoum, Sudan
Badawi Ahmed Zakaria
Central Laboratory, Ministry of Science and Technology, Khartoum, Sudan
Abdalla Ahmed Elbashir
University of Khartoum, Khartoum, Sudan
ABSTRACT
Simple High Performance Liquid Chromatographic (HPLC) method was validated for determination of the content levels caffeine in 15 different beverage samples commercially available in Sudan local markets. Shim-pack VP-ODS column was used with methanol: water (30:70)% (v/v) eluent. The detector wavelength was set at 270 nm. Linearity of the method was check from 10-100 ppm and the correlation coefficient was 0.9999. The method detection limit was 0.023 ppm and the precision was 1.25% at 40 ppm caffeine concentration.. the spiked recoveries for caffeine were 99%, 105%, 99.2%, 102% and 102% in Pepsi Cola, Coca Cola, Red Bull, Gazaltain black tea and Coffee samples respectively. The caffeine contents in tea samples ranged from 440 ppm to 473 ppm with average concentration of 458.6 ppm. The caffeine concentrations in energy drinks samples ranged from 170.6 ppm to 324 ppm with average concentration of 255.6 ppm. The coffee sample contains 252.4 ppm. The carbonated soft drinks showed caffeine content in the range of 32.4 ppm to 133.3 ppm with average concentration of 96 ppm. In addition, the concentrations of caffeine have been converted into the daily intake doses based on beverages consumption. The mean values of caffeine daily intakes were 183 mg, 101 mg, 64 mg and 38 mg through the ingestion of tea, coffee, energy drinks and soft drinks, respectively.
PDF References Citation
How to cite this article
Mei Musa Ali, Mawahib Eisa, Mohammed Idrees Taha, Badawi Ahmed Zakaria and Abdalla Ahmed Elbashir, 2012. Determination of Caffeine in Some Sudanese Beverages by High Performance
Liquid Chromatography. Pakistan Journal of Nutrition, 11: 336-342.
DOI: 10.3923/pjn.2012.336.342
URL: https://scialert.net/abstract/?doi=pjn.2012.336.342
DOI: 10.3923/pjn.2012.336.342
URL: https://scialert.net/abstract/?doi=pjn.2012.336.342
REFERENCES
- Alpdogan, G., K. Karabina and S. Sungur, 2002. Derivative spectrophotometric determination of caffeine in some beverages. Turk. J. Chem., 26: 295-302.
Direct Link - Ashihara, H., A.M. Monteiro, M.F. Gillies and A. Crozier, 1996. Biosynthesis of caffeine in leaves of coffee. Plant Physiol., 111: 747-753.
Direct Link - Komes, D., D. Horzic, A. Belscak, K.K. Ganic and A. Baljak, 2009. Determination of caffeine content in tea and mate tea by using different methods. Czech. J. Food Sci., 27: 213-216.
Direct Link - Lane, J.D., R.A. Adcock, R.B. Williams and C.M. Kuhn, 1990. Caffeine effects on cardiovascular and neuroendocrine responses to acute psychosocial stress and their relationship to level of habitual caffeine consumption. Psychosomatic Med., 52: 320-336.
PubMed - Ling, L.S., N.I.N. Daud and O. Hassan, 2001. Determination of coffee content in coffee mixtures. Malaysian J. Anal. Sci., 7: 327-332.
Direct Link - Bispo, M.S., C.M.C. Velosol, C.H.L. Pinheiro, F.S.R. De Oliveira, N.J.O. Reis and B.J. De Andrade, 2002. Simultaneous determination of caffeine, theobromine and theophylline by high-performance liquid chromatography. J. Chromatogr. Sci., 40: 45-48.
PubMed - Delaney, M.F., M.K. Pasko, M.D. Mauro, S.D. Gsell and C.P. Korologos et al., 1985. Determination of aspartame, caffeine, saccharin and benzoic acid in beverages by high performance liquid chromatography. J. Chem. Edu., 62: 618-620.
Direct Link - Tavallali, H. and M. Sheikhaei, 2009. Simultaneous kinetic determination of paracetamole and caffeine using Cu(II)-neocuproine in presence of dodecyl sulphate by H-point standard addition method. In. J. Chem., 48A: 812-816.
Direct Link - Nour, V., I. Trandafir and M.E. Ionica, 2010. Chromatographic determination of caffeine contents in soft and energy drinks available on the Romanian market. Sci. Study Res. Chem. Chem. Eng. Biotechnol. Food Ind., 11: 351-358.
Direct Link - Wanyika, H.N., E.G. Gatebe, L.M. Gitu, E.K. Ngumba and C.W. Maritim, 2010. Determination of caffeine content of tea and instant coffee brands found in the Kenyan market. Afr. J. Food Sci., 4: 353-358.
Direct Link