J. Ahmed
Department of Microbiology, University of Chittagong, Bangladesh
U. M. Seraj
Department of Microbiology, University of Chittagong, Bangladesh
M. A. Chowdhury
Chittagong Maa-0 Shishu (Children) Hospital, Chittagong, Bangladesh
S. Chowdhury
Department of Microbiology, University of Chittagong, Bangladesh
ABSTRACT
Molecular biology techniques were used for quick diagnosis of thalassemia in the present study. The incidence of thalassemia markers of both sexes, aged between 0.5-44 years, living in different areas of Chittagong, Bangladesh was studied. Information was collected from the subjects by questionnaire to find out the relationship of the thalassemia with sex, age, present illness, post illness, family relationships, personal history etc. Serum sample from the individuals were tested for routine hematological examination test and using molecular biology technique. Out of 45 subjects tested, a total of 29 cases (64.44%) were found to be thalassemia positive. Analysis of thalassemia markers in different sex groups showed that the incidence of thalassemia is greater in male (65.51%) than female (34.48%). The study also showed that thalassemia major were lower (34.48%) than the thalassemia minor (65.51%). Most of them are dependent on blood transfusion for their lives. It is concluded that the disease could be diagnosed quickly by electrophoresis and most important way to prevent the disease is to detect thalassemia carriers, parental diagnosis and pre-marriage counseling.
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How to cite this article
J. Ahmed, U. M. Seraj, M. A. Chowdhury and S. Chowdhury, 2004. An Epidemiological Study of Thalassemia, its Quick Diagnosis and Influence of Malaria in Chittagong Area of Bangladesh. Pakistan Journal of Biological Sciences, 7: 1953-1957.
DOI: 10.3923/pjbs.2004.1953.1957
URL: https://scialert.net/abstract/?doi=pjbs.2004.1953.1957
DOI: 10.3923/pjbs.2004.1953.1957
URL: https://scialert.net/abstract/?doi=pjbs.2004.1953.1957
REFERENCES
- Dozy, A.M., 1979. Prenatal diagnosis of homozygous α thalassemia. J. Am. Med. Assoc., 241: 1610-1612.
CrossRef - Loosekoot, R., R. Fodde and C.L. Hartveld, 1990. Denaturing gradient gel electrophoresis (D.G.G.E) and direct sequencing of PCR amplified genomic DNA: A rapid and reliable diagnostic approach to β thalassemia. Br. J. Hematol., 76: 269-274.
Direct Link - Mazza, U., 1976. Clinical and hematological data in 254 cases of β thalassemia trait in Italy. Br. J. Hematol., 33: 91-99.
CrossRef - Kulozik, A.E., B.C. Kar, R.K. Satapathy, B.E. Serjeant, G.R. Serjeant and D.J Weatherall 1987. Foetal hemoglobin levels and Bs globin haplotypes in an Indian population with sickle cell disease. Blood, 69: 1742-1748.
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