Hidayet Bostan
Department of Plant Protection, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey
Erkol Demirci
Department of Plant Protection, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey
ABSTRACT
This study was conducted to obtain virus-free propagation materials from Granola, Pasinler 92 and Caspar potato (Solanum tuberosum L.) cultivars infected with potato virus X (PVX), potato virus Y (PVY) and potato leaf roll virus (PLRV) by using meristem-tip culture. For in vitro propagation, it was tested the effect of different combinations and concentrations of benzylamino purine (BA) (0.0, 0.25, 0.50 mg L-1) and gibberellic acid (GA3) (0.0, 0.25, 0.50 mg L-1) on the number of shoot and node. On the other hand, it was evaluated the effect of BA (0.00, 5.00, 10.0 mg L-1) and CCC (chlorocholine chloride) (0.00, 500 mg L-1) on the tuberization under two photoperiodic regimes (light and dark). The MS salts and vitamins supplemented with 30 g L-1 sucrose was used as a medium and the media was solidified with 7.0 g L-1 agar and the ratio of sucrose added into media for micro tuber production had been increased from 3-8%. The highest number of shoots was obtained from 0.00/0.25, 0.25/0.50 and 0.00/0.00 mg L-1 BA/GA3 treatments for Granola, Pasinler 92 and Caspar cultivars as 1.52, 1.24 and 1.44, respectively. However, the highest number of node were determined on 0.00/0.50 for Granola (9.12), Pasinler 92 (8.76) and on 0.00/0.25 mg L-1 BA/GA3 treatments for Caspar (8.24). When the results were assayed according to total tuber number, the most micro-tubers for Granola, Pasinler 92 and Caspar cultivars were obtained from 5.00/5000 mg L-1 BA/CCC treatment as 5.6, 4.0, 4.8 per/bottle under dark treatments. All in vitro regenerated plant materials were tested by DAS-ELISA (double antibody sandwich enzyme-linked immunosorbent assay) to determine the presence and absence of viruses and PVX, PVY and PLRV viruses were eliminated from Granola (25, 40 and 60%), Pasinler 92 (16, 41.6 and 46.1%) and Caspar cultivars (28.5, 33.3 and 50%), respectively.
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How to cite this article
Hidayet Bostan and Erkol Demirci, 2004. Obtaining PVX, PVY and PLRV-Free Micro Tuber from Granola, Pasinler 92 and
Caspar Potato (Solanum tuberosum L.) Cultivars. Pakistan Journal of Biological Sciences, 7: 1135-1139.
DOI: 10.3923/pjbs.2004.1135.1139
URL: https://scialert.net/abstract/?doi=pjbs.2004.1135.1139
DOI: 10.3923/pjbs.2004.1135.1139
URL: https://scialert.net/abstract/?doi=pjbs.2004.1135.1139
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