Beneficial Role of Lactic Acid Bacteria in Food Preservation and Human Health: A Review
Asmahan Azhari Ali
There is several potential health or nutritional benefits possible from some species of lactic acid bacteria. Among these are: improved nutritional value of food, control of intestinal infections, improved digestion of lactose, control of some types of cancer and control of serum cholesterol levels. Some potential benefits may result from growth and action of the bacteria during the manufacture of cultured foods. Some may result from growth and action of certain species of the lactic acid bacteria in the intestinal tract following ingestion of foods containing them. In selecting a culture to produce a specific benefit it is necessary to consider not only the wide variation among species of the lactic acid bacteria but also that among strains within a given species. The production of fermented foods is based on the use of starter cultures, for instance lactic acid bacteria that initiate rapid acidification of the raw material. Recently, new starter cultures of lactic acid bacteria with an industrially important functionality are being developed. The latter can contribute to the microbial safety or offer one or more organoleptic, technological, nutritional, or health advantages. Examples are lactic acid bacteria that produce antimicrobial substances, sugar polymers, sweeteners, aromatic compounds, vitamins, or useful enzymes, or that have probiotic properties. In the developed world, lactic acid bacteria are usually associated with fermented dairy products and the use of dairy starter cultures has become a whole industry this century. Add to this that lactic acid bacteria have been associated with beneficial health effects for many years now. Nowadays, many food products are promoted as being particularly healthy because of the characteristics of certain strains of lactic acid bacteria. Unfortunately, not all these strains have been studied carefully and therefore it might be difficult to substantiate some of the claims. Thus, there is a clear need for the critical study of the effects on health of strain selectio and quality.
May 19, 2010; Accepted: June 02, 2010;
Published: July 01, 2010
Lactic Acid Bacteria (LAB) are gram positive, typically non- sporulating rod
or coccus shaped. They lack catalase and strictly fermentative, producing either
a mixture of lactic acid, carbon dioxide, acetic acid and/or ethanol (heterofermentation)
or almost entirely lactic acid (homofermentation) as the major metabolic end-product
(Rogosa, 1974; Collins and Lyne, 1984;
Jay, 1986; Kandler and Weiss, 1986;
Schillinger and Lucke, 1987; Priest
and Campbell, 1996). Kluyver divided LAB into two groups based on the end
product of glucose metabolism; those that produce lactic acid as the only product
of fermentation are designated homofermentative. The homofermentative pattern
is observed when glucose is metabolized but not necessarily when pentose sugars
are metabolized, for some, homolactics produce acetic acids when utilizing pentose.
Also the homofermentative character of homolactics may be shifted for some strains
by altering cultural conditions such as glucose concentration, pH and nutrient
limitation. The homolactics able to extract twice as much energy from a given
quantity of glucose as are the heterolactics. These lactics that produce equal
molar amounts of lactate, carbon dioxide and ethanol from hexoses are designated
heterofermentative. All members of the genera Pediococcus, Streptococcus,
Lactococcus and Vagococcus are homofermenters, along with some
of the lactobacilli, while all Leuconostoc sp., as well as some
Lactobacilli are heterofermenters. The heterolactics are more important
than the homolactics in producing flavour and aroma components such as acetylaldehyde
and diacetyl (Sharpe, 1979; Jay, 1986;
Schillinger and Lucke, 1987).
LAB IN BIOCONTROL AND FERMENTATION
Biological Control of Food Borne Pathogens
Research has focused on the biological approach to the control and eradication
of food borne pathogens. Scientists developed natural antimicrobial products
for the biocontrol of pathogens and have exploited LAB for the competitive exclusion
of pathogens and delivery of vaccines and bioactive compounds (Grasson,
LAB in Competitive Exclusion
The gastrointestinal tract of humans and animals contain a complex bacterial
ecosystem. Commensal strains of LAB have a history of use with the intention
of enhancing health in the form of probiotics and controlling human pathogens
in farm animals. Research has demonstrated the capacity of Lactobacillus
species to control arrange of human pathogens including E. coli,
Compylobacter jejuni and Clostridium perfringes (Grasson,
LAB as Probiotics
In discussing the importance of Lactobacillus species in fermented
foods one also needs to consider their importance as probiotics was later defined
as a live microbial feed supplement, which is beneficial to the host animal
through improving its intestinal microbial balance (Steinkrause,
1995). Lactobacillus sp. has been used as probiotic organisms. In
this case, L. acidophilus has been used because it was thought
to be the dominant lactobacillus in the intestine. However, a wide range
of lactobacilli has been used in probiotic preparations. These include:
L. delbreuckii subsp. bulgaricus, L. casei, L. brevis,
L. cellobiosus, L. lactis, L. fermentum, L. plantarum
and L. reuteri (Steikrause, 1995; Vinderola et
al., 2002). Metchnikoff (1908) described the beneficial
effect of lactic acid bacteria on human health almost a century ago. Although,
numerous studies have substantiated the findings of Metchnikoff, it has been
a difficult scientific discipline to identify and prove the mode of action for
probiotics (Mattila-Sandholm et al., 1999; Pathmakanthan
et al., 2000). The pre-existing flora of the digestive tract is
complex and ill-defined which makes it very difficult to determine how probiotics
influence the intestinal ecosystem (Tannock, 1998; Kleessen
et al., 2000; Macfarlane et al., 2000).
The presence of microbial flora is necessary for the normal function of the
digestive system. Elimination or severe perturbations of the flora leads to
diarrhoea or constipation and the maintenance of a healthy bacterial flora is
therefore desirable (Tannock, 1998; Pathmakanthan
et al., 2000). In the absence of precise models for the mode of action,
a number of practical criteria for selecting probiotic strains have been formulated
(Collins et al., 1998; Salminen
et al., 2000; Klaenhammer and Kullen, 1999;
Mattila-Sandholm et al., 1999). The large increase
in the occurrence of allergy in the populations of the industrialized world
is still largely unexplained. One of several hypothesizes is the hygiene hypothesis,
which explains the increase by our modern environment being too aseptic (Martinez
and Holt, 1999; Van den Biggelaar et al., 2000).
If this is, indeed, a part of the problem, we will need to design our future
fermented food or the probiotic products to contain safe microorganisms counteracting
this unwanted distortion of the immune system.
LAB as Vaccine Delivery Vehicles
Commensal LAB can be exploited to deliver vaccines and other biological
active material to the gastrointestinal tract. Their use in vaccine delivery
is of special value in stimulating mucosal immunity that is protective at the
site of pathogen entry. The advantages of LAB delivery include: ease of administration;
survival in stomach acid; inherent safety; particulate nature and size for uptake
by cells; economic technology in that the bacterial manufacture the vaccine
or therapeutic agent (Grasson, 2002).
LAB as Beneficial Microorganisms
The LAB are important commercially in the processing of meats, alcoholic
beverages and vegetables. The products include sausages, cured hams, wines,
beer, fortified spirits, pickles and sauerkraut (Collins and
Lyne, 1976; Sharpe, 1981; Jay,
1986; Kandler and Weiss, 1986; Hastings
and Holzapfel, 1987; Schillinger and Lucke, 1987;
Jay, 1992). Although, LAB have beneficial effects in the
food industry, they can sometimes be a nuisance as contaminants by producing
off flavours (Kandler and Kunath, 1983; Aguirre
and Collins, 1993; Cai et al., 1998). Lactobacillus
and Streptococcus faecum are beneficial microorganisms, which have been
proven to replenish essential microflora and decrease the incidence of gastrointestinal
disorders. Beneficial bacteria, especially lactobacillus sp. can produce
anti- microbial substances, which have been observed to inhibit the growth of
some pathogenic microorganisms. The addition of type O lactic culture may be
an additional safeguard to established good manufacturing practices and Hazard
Analysis and Critical Control Point (HACCP) programs in the control of growth
of E. coli 0157:H7 in minas cheese (Saad et al.,
2001; Yost and Nattress, 2002). These beneficial
microorganisms are most effective during periods of disease or stress and following
Importance of LAB and their Effect on Human Health
Of interest is the role of LAB in the treatment of people suffering with
tumors and immune compromised subjects. The evidence that LAB can stimulate
the immune system is remarkable and fascinating in it and opens many questions
about mechanisms and effective utilization. If this potential is supported in
practice, then there are many components to conventional therapies. This may
include cost effectively due to their ease of products derived from LAB seem
to have relatively low toxicity compared to other treatments (Wood,
Lactic Acid Bacteria and Other Effects on the Immune System
The LAB are present in the intestine of most animals. The beneficial role
played by this microorganism in humans and animals, including the effect on
the immune system has been extensively reported (Perdigon
et al., 2001). The LAB are present in many foods and are frequently
used as probiotics to improve some biological functions in the host. Through
different mechanisms they send signals to active immune cells. Thus the knowledge
of the normal intestinal microflora, the contribution of LAB and their role
in the numerous functions in the digestive tract as well as the functioning
of the mucosal immune system. In the selection of LAB by their immune stimulatory
capacity, it helps to know not only the effect which they have on the mucosal
immune system, but the specific use to which these oral vaccine vectors are
being put (Perdigon et al., 2001).
LAB as Starter Culture
Lactic Acid Bacteria (LAB) are the most important bacteria used in food
fermentations. Apart from general demands for starter cultures from the view
of safety, technological effectiveness and economics, numerous specific aspects
have to be considered when selecting strains for the different food fermentations.
Therefore, selection criteria for LAB depend on the type and the desired characteristics
of the final product, the desired metabolic activities, the characteristics
of the raw materials and the applied technology. A starter culture can be defined
as a microbial preparation of large numbers of cells of at least one microorganism
to be added to a raw material to produce a fermented food by accelerating and
steering its fermentation process. The group of Lactic Acid Bacteria (LAB) occupies
a central role in these processes and has a long and safe history of application
and consumption in the production of fermented foods and beverages (Caplice
and Fitzgerald, 1999; Ray, 1992; Wood,
1997; Wood and Holzapfel, 1995) (Table
1). They cause rapid acidification of the raw material through the production
of organic acids, mainly lactic acid. Also, their production of acetic acid,
ethanol, aroma compounds, bacteriocins, exopolysaccharides and several enzymes
is of importance. In this way they enhance shelf life and microbial safety,
improve texture and contribute to the pleasant sensory profile of the end product.
|| Fermented foods and beverages and their associated lactic
|B: Bifidobacterium, C: Carnobacterium, L: Lactococcus,
Lb: Lactobacillus, Leuc: Leuconostoc, O: Oenococcus,
P: Pediococcus, S: Streptococcus, T: Tetragenococcus,
The earliest production of fermented foods was based on spontaneous fermentation
due to the development of the microflora naturally present in the raw material.
The quality of the end product was dependent on the microbial load and spectrum
of the raw material. Spontaneous fermentation was optimized through back-slopping,
i.e., inoculation of the raw material with a small quantity of a previously
performed successful fermentation. Hence, back-slopping results in dominance
of the best adapted strains. It represents a way, be it unconsciously, of using
a selected starter culture to shorten the fermentation process and to reduce
the risk of fermentation failure. Back-slopping is still in use, for instance
in the production of sauerkraut and sourdough and particularly for products
for which the microbial ecology and the precise role of successions in microbial
population are not well known (Harris, 1998). Today,
the production of fermented foods and beverages through spontaneous fermentation
and back-slopping represents a cheap and reliable preservation method in less
developed countries, whereas in Western countries the large-scale production
of fermented foods has become an important branch of the food industry. Moreover,
the Western consumer appreciates traditionally fermented products for their
outstanding gastronomic qualities. The direct addition of selected starter cultures
to raw materials has been a breakthrough in the processing of fermented foods,
resulting in a high degree of control over the fermentation process and standardization
of the end product. Strains with the proper physiological and metabolic features
were isolated from natural habitats or from successfully fermented products
(Oberman and Libudzisz, 1998). However, some disadvantages
have to be considered. In general, the initial selection of commercial starter
cultures did not occur in a rational way, but was based on rapid acidification
and phage resistance. These starters are not very flexible with regard to the
desired properties and functionality of the end product. Originally, industrial
starter cultures were maintained by daily propagation. Later, they became available
as frozen concentrates and dried or lyophilized preparations, produced on an
industrial scale, some of them allowing direct vat inoculation (Sandine,
1996). Because the original starter cultures were mixtures of several undefined
microbes, the daily propagation probably led to shifts of the ecosystem resulting
in the disappearance of certain strains. Moreover, some important metabolic
traits in LAB are plasmid-encoded and there is a risk that they are lost during
propagation. It is further likely that loss of genetic material occurred due
to adaptation to the food matrix. The biodiversity of commercial starters has
therefore become limited. This often leads to a loss of the uniqueness of the
original product and the loss of the characteristics that have made the product
popular (Caplice and Fitzgerald, 1999). In contrast,
the fermentation of traditional fermented foods is frequently caused by natural,
wild-type LAB that originate from the raw material, the process apparatus, or
the environment and that initiate the fermentation process in the absence of
an added commercial starter (Bocker et al., 1995;
Weerkamp et al., 1996). Moreover, many traditional
products obtain their flavour intensity from the Non-Starter Lactic Acid Bacteria
(NSLAB), which are not part of the normal starter flora but develop in the product,
particularly during maturation, as a secondary flora (Beresford
et al., 2001). Pure cultures isolated from complex ecosystems of
traditionally fermented foods exhibit a diversity of metabolic activities that
diverge strongly from the ones of comparable strains used as industrial bulk
starters (Klijn et al., 1995). These include
differences in growth rate and competitive growth behavior in mixed cultures,
adaptation to a particular substrate or raw material, antimicrobial properties
and flavour, aroma and quality attributes. Wild strains need to withstand the
competition of other microorganisms to survive in their hostile natural environment,
so that they often produce antimicrobials such as bacteriocins (Ayad
et al., 2002). In addition, they are more dependent
on their own biosynthetic capacity than industrial strains and harbour more
amino acid converting enzymes that play a key role in flavour formation. Such
findings underline the importance of the Designation of Protected Origin (DPO)
of many of these products, which is crucial from an economical point of view
since they contribute to the survival of small-scale fermentation plants in
a world of ongoing globalization. A recent trend exists in the isolation of
wild-type strains from traditional products to be used as starter cultures in
food fermentation (Beukes et al., 2001; De
Vuyst et al., 2002; Hebert et al., 2000).
Many indigenous cereal fermentations involve the combine action of bacteria
and yeast. L. fermentum and L. amylovorus have been suggested
to be the predominating microorganisms during fermentation of sorghum dough
in Sudanese Kisra (Asmahan and Muna, 2009), also the
use of Lactobacillus starter in sorghum flour fermentation decreased
the traditional fermentation time from 19 to 6 h, the thing that might contribute,
in no small way, in encouraging the production and development of more sorghum
based products. Using yeast during Lactobacillus fermentation of sorghum
has, furtherly, shortened the fermentation time to 4 h. The pH was decreased
to 3.7 (Asmahan, 2007). Indigenous fermented food represents
a unique source for future application in food technology. Little or no scientific
information on lactic acid bacteria in certain indigenous, fermented sorghum
products in Africa, Kisra is a prime example of such a product.
Lactic acid bacteria (LAB) have health-promoting attributes, including antimutagenic and anticarcinogenic activities, hypocholesterolemic properties and antagonistic actions that can restrain intestinal and food borne pathogens and immune modulation effects. The LAB have been reported to improve gastrointestinal health. Furthermore, LAB and their fermented products can effectively enhance the integrity of the gastric mucosa. Many reports suggest that LAB and their fermented products have protective effects against mucosal injury in the stomach.
The LABS are typically involved in a large number of spontaneous food fermentations but they are also closely associated with the human environment. Food fermentations have a great economic value and it has been accepted that these products contribute in improving human health. The LABS have contributed in the increased volume of fermented foods world wide especially in foods containing probiotics or health promoting bacteria. Bacteriocins produced by LAB are the subject of intense research because of their antibacterial activity against food borne bacteria. Further studies should be focused on the mechanisms of action of LAB within the gastro intestinal tract and in the immune system which stimulate the in vivo immunity effects. Furthermore, genetic engineering of already identified probiotics and those newly discovered to make them more efficacious should be pursued.
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